Chapter 25, Problem 25.15P

a)

Interpretation Introduction

Interpretation:

The type of liquid chromatography that is used to separate a compound with molecular mass < 2000 with solubility in Octane has to be identified.

Concept Introduction:

Liquid Chromatography:

Liquid Chromatography is used for the separation of sample to its individual parts. The separation happens based on the interactions of the sample with mobile and stationary phase.

HPLC is High Pressure Liquid Chromatography.  Here, it is same as liquid chromatography but the difference is that high pressure is used.  This method uses small columns and particle size.  By using HPLC, one can identify, separate and quantitate the components that is present in the given sample.

High Pressure Liquid Chromatography uses high pressure to generate the flow of mobile phase in the packed columns.  As the packed columns are small in size and the particles that are packed in the column also is present in the order of micrometers, high pressure is required to generate flow of the mobile phase into the stationery phase.  Therefore, high pressure is required in HPLC.

b)

Interpretation Introduction

Interpretation:

The type of liquid chromatography that is used to separate a compound with molecular mass < 2000 with solubility in Methanol-Water mixture has to be identified.

Concept Introduction:

Liquid Chromatography:

Liquid Chromatography is used for the separation of sample to its individual parts. The separation happens based on the interactions of the sample with mobile and stationary phase.

HPLC is High Pressure Liquid Chromatography.  Here, it is same as liquid chromatography but the difference is that high pressure is used.  This method uses small columns and particle size.  By using HPLC, one can identify, separate and quantitate the components that is present in the given sample.

High Pressure Liquid Chromatography uses high pressure to generate the flow of mobile phase in the packed columns.  As the packed columns are small in size and the particles that are packed in the column also is present in the order of micrometers, high pressure is required to generate flow of the mobile phase into the stationery phase.  Therefore, high pressure is required in HPLC.

c)

Interpretation Introduction

Interpretation:

The type of liquid chromatography that is used to separate a compound with molecular mass < 2000 ,weak acid has to be identified.

Concept Introduction:

Liquid Chromatography:

Liquid Chromatography is used for the separation of sample to its individual parts. The separation happens based on the interactions of the sample with mobile and stationary phase.

HPLC is High Pressure Liquid Chromatography.  Here, it is same as liquid chromatography but the difference is that high pressure is used.  This method uses small columns and particle size.  By using HPLC, one can identify, separate and quantitate the components that is present in the given sample.

High Pressure Liquid Chromatography uses high pressure to generate the flow of mobile phase in the packed columns.  As the packed columns are small in size and the particles that are packed in the column also is present in the order of micrometers, high pressure is required to generate flow of the mobile phase into the stationery phase.  Therefore, high pressure is required in HPLC.

d)

Interpretation Introduction

Interpretation:

The type of liquid chromatography that is used to separate a compound with molecular mass < 2000, highly polar has to be identified.

Concept Introduction:

Liquid Chromatography:

Liquid Chromatography is used for the separation of sample to its individual parts. The separation happens based on the interactions of the sample with mobile and stationary phase.

HPLC is High Pressure Liquid Chromatography.  Here, it is same as liquid chromatography but the difference is that high pressure is used.  This method uses small columns and particle size.  By using HPLC, one can identify, separate and quantitate the components that is present in the given sample.

High Pressure Liquid Chromatography uses high pressure to generate the flow of mobile phase in the packed columns.  As the packed columns are small in size and the particles that are packed in the column also is present in the order of micrometers, high pressure is required to generate flow of the mobile phase into the stationery phase.  Therefore, high pressure is required in HPLC.

e)

Interpretation Introduction

Interpretation:

The type of liquid chromatography that is used to separate a compound with molecular mass < 2000, ionic has to be identified.

Concept Introduction:

Liquid Chromatography:

Liquid Chromatography is used for the separation of sample to its individual parts. The separation happens based on the interactions of the sample with mobile and stationary phase.

HPLC is High Pressure Liquid Chromatography.  Here, it is same as liquid chromatography but the difference is that high pressure is used.  This method uses small columns and particle size.  By using HPLC, one can identify, separate and quantitate the components that is present in the given sample.

High Pressure Liquid Chromatography uses high pressure to generate the flow of mobile phase in the packed columns.  As the packed columns are small in size and the particles that are packed in the column also is present in the order of micrometers, high pressure is required to generate flow of the mobile phase into the stationery phase.  Therefore, high pressure is required in HPLC.

f)

Interpretation Introduction

Interpretation:

The type of liquid chromatography that is used to separate a compound with molecular mass < 2000, soluble in Water, non-ionic and various sized solutes has to be identified.

Concept Introduction:

Liquid Chromatography:

Liquid Chromatography is used for the separation of sample to its individual parts. The separation happens based on the interactions of the sample with mobile and stationary phase.

HPLC is High Pressure Liquid Chromatography.  Here, it is same as liquid chromatography but the difference is that high pressure is used.  This method uses small columns and particle size.  By using HPLC, one can identify, separate and quantitate the components that is present in the given sample.

High Pressure Liquid Chromatography uses high pressure to generate the flow of mobile phase in the packed columns.  As the packed columns are small in size and the particles that are packed in the column also is present in the order of micrometers, high pressure is required to generate flow of the mobile phase into the stationery phase.  Therefore, high pressure is required in HPLC.

g)

Interpretation Introduction

Interpretation:

The type of liquid chromatography that is used to separate a compound with molecular mass < 2000, soluble in Water, variety of charges has to be identified.

Concept Introduction:

Liquid Chromatography:

Liquid Chromatography is used for the separation of sample to its individual parts. The separation happens based on the interactions of the sample with mobile and stationary phase.

HPLC is High Pressure Liquid Chromatography.  Here, it is same as liquid chromatography but the difference is that high pressure is used.  This method uses small columns and particle size.  By using HPLC, one can identify, separate and quantitate the components that is present in the given sample.

High Pressure Liquid Chromatography uses high pressure to generate the flow of mobile phase in the packed columns.  As the packed columns are small in size and the particles that are packed in the column also is present in the order of micrometers, high pressure is required to generate flow of the mobile phase into the stationery phase.  Therefore, high pressure is required in HPLC.

h)

Interpretation Introduction

Interpretation:

The type of liquid chromatography that is used to separate a compound with molecular mass < 2000, soluble in Tetrahydrofuran has to be identified.

Concept Introduction:

Liquid Chromatography:

Liquid Chromatography is used for the separation of sample to its individual parts. The separation happens based on the interactions of the sample with mobile and stationary phase.

HPLC is High Pressure Liquid Chromatography.  Here, it is same as liquid chromatography but the difference is that high pressure is used.  This method uses small columns and particle size.  By using HPLC, one can identify, separate and quantitate the components that is present in the given sample.

High Pressure Liquid Chromatography uses high pressure to generate the flow of mobile phase in the packed columns.  As the packed columns are small in size and the particles that are packed in the column also is present in the order of micrometers, high pressure is required to generate flow of the mobile phase into the stationery phase.  Therefore, high pressure is required in HPLC.