a)
Interpretation:
The volume
b)
Interpretation:
The order of elution has to be explained.
c)
Interpretation:
The retention factor for each solute has to be calculated.
Concept Introduction:
Retention factor:
The time that is necessary to elute that peak minus the minimum possible time for the travel of unretained solute through the column is called as retention factor. The retention factor is calculated using the equation,
Where,
d)
Interpretation:
The order of elution has to be explained and the reason why the gradient goes from high to low Acetonitrile volume fraction has to be given.
To explain the order of elution
To give the reason why the gradient goes from high to low Acetonitrile volume fraction
e)
Interpretation:
The value of
To estimate the value of
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