Chapter 26, Problem 26.CE

(a)

Interpretation Introduction

Interpretation:

The separation of Blue Dextran has 2000 in gel filtration column using should be explained.

Concept introduction:

Gel filtration in open columns:

In the gel filtration column, the pore size of the stationary phase is chosen depends on the molecular mass of the molecules to be separated.

The fractionation range for globular proteins (Da) are used as gel in gel filtration columns and more size of gel are available based on it is size so the stationary phase size (Da) is depends on molar mass of the compound, which is going to separate.

(b)

Interpretation Introduction

Interpretation:

The retention volume of a molecule in gel-filtration column should be determined.

Concept introduction:

The volume of column is given by the mathematical expression it is,

${\text{V}}_{\text{t}}\text{=}{\text{πr}}^{\text{2}}\text{l}$

Where,

${\text{V}}_{\text{f}}$ is volume of column

$\pi$ is 3.14

$\text{r}$ is radius of the column

$\text{l}$ is length of the column

The Elution Equation:

${\text{K}}_{\text{av}}\text{=}\frac{{\text{V}}_{\text{r}}{\text{-V}}_{\text{0}}}{{\text{V}}_{\text{m}}{\text{-V}}_{\text{0}}}$

Where,

${\text{V}}_{\text{m}}$ is total volume of mobile phase.

${\text{V}}_{\text{r}}$ is volume of known solute.

${\text{V}}_{\text{0}}$ is initial volume.