Inquiry into Life
16th Edition
ISBN: 9781260482638
Author: Mader, Sylvia
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Textbook Question
Chapter 26.1, Problem 2CYP
Explain how the PCR reaction amplifies a segment of DNA.
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Explain how PCR eventually generates a discrete-sized fragment from a much longer piece of DNA.
b) Describe how DNA is digested by different restriction enzymes
c) Describe how gel electrophoresis is used to estimate the size of DNA fragments.
Describe the method of a PCR technique in which you can amplify fragments randomly? Briefly.
Chapter 26 Solutions
Inquiry into Life
Ch. 26.1 - Describe the steps in forming Recombinant DNA.Ch. 26.1 - Discuss how the polymerase chain reaction works.Ch. 26.1 - Prob. 3LOCh. 26.1 - Prob. 1QTCCh. 26.1 - Prob. 2QTCCh. 26.1 - Prob. 1CYPCh. 26.1 - Explain how the PCR reaction amplifies a segment...Ch. 26.1 - Prob. 3CYPCh. 26.1 - Prob. 1AQTCCh. 26.1 - Prob. 2AQTC
Ch. 26.2 - Prob. 1LOCh. 26.2 - Prob. 2LOCh. 26.2 - Prob. 1QTCCh. 26.2 - Prob. 2QTCCh. 26.2 - Prob. 3QTCCh. 26.2 - Prob. 1CYPCh. 26.2 - Prob. 2CYPCh. 26.3 - Compare and contrast in vivo and ex vivo gene...Ch. 26.3 - Prob. 2LOCh. 26.3 - Prob. 1CYPCh. 26.3 - Prob. 2CYPCh. 26.3 - Prob. 3CYPCh. 26.4 - Prob. 1LOCh. 26.4 - Prob. 2LOCh. 26.4 - Prob. 3LOCh. 26.4 - Prob. 1CYPCh. 26.4 - Explain how comparative genomics can provide...Ch. 26.4 - Prob. 3CYPCh. 26 - Prob. S25.1BYBCh. 26 - Prob. S25.2BYBCh. 26 - Prob. S25.3BYBCh. 26 - Prob. 1ACh. 26 - Prob. 2ACh. 26 - The polymerase chain reaction Use RNA polymerase...Ch. 26 - Prob. 4ACh. 26 - Prob. 5ACh. 26 - In this process, cells are removed from the body...Ch. 26 - When a cloned gene is used to modify a human...Ch. 26 - Prob. 8ACh. 26 - Prob. 9ACh. 26 - Prob. 10ACh. 26 - Prob. 1TCCh. 26 - In a genomic comparison between humans and yeast,...
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- Describe the activities of the enzymes required to construct a recombinant DNA molecule.arrow_forwardExplain what restriction enzymes are, how they function and how they can be used to make recombinant DNAarrow_forwardMost PCR reactions do not use the more expensive types of DNA polymerase, which have DNA proofreading. How might this be a problem in accurately copying specific DNA sequences of the target gene?arrow_forward
- Outline the steps by which the polymerase chainreaction (PCR) amplifies a specific region of a genome.arrow_forwardExplain how recombinant DNA technology uses restriction enzymes (restriction endonucleases). Explain how recombinant DNA technology uses DNA ligase enzymes.arrow_forwardExplain why the precise length of the template DNA sequence does not become amplified until the third cycle. Draw a picture that explains your answer. For: Electrophoresisarrow_forward
- A restriction enzyme digests DNA into fragments.term the technique used to check the progression of this enzyme and separate DNA fragments.arrow_forwardDetermine the gene sequence that is amplified by the use of primers in the polymerase chain reaction.arrow_forwardExplain why exome sequencing can be almost as valuable as genome sequencing.arrow_forward
- Describe the automated dideoxy chain termination method of DNA sequencing.arrow_forwardExplain how restriction enzymes recognize and digest DNA to create either blunt or sticky endsarrow_forwardDefine PCR. Write down different types of PCR in detail with their applications. Give the answer broadly.arrow_forward
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