Concept explainers
(a)
Interpretation:
Histidine at
Concept Introduction:
On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.
(b)
Interpretation:
Lysine at
Concept Introduction:
On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.
(c)
Interpretation:
Glutamic acid at
Concept Introduction:
On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.
(d)
Interpretation:
Glutamine at
Concept Introduction:
On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.
(e)
Interpretation:
Glu-Ile-Val at
Concept Introduction:
On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.
(f)
Interpretation:
Lys-Gln-Tyr at
Concept Introduction:
On electrophoresis, the amino acids which contains net positive charge will move towards the negative electrode and the amino acids which contain net negative charge will move towards positive electrode.
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Chapter 27 Solutions
Organic Chemistry
- Hemoglobin A has a pI value of 5.6 whereas the variant hemoglobin M has an aspartate residue in place of the normal valine at position 85 of the alpha chain. What effect will this substitution have on the electrophoretic behavior of the protein at pH 7.0. (explain clearly the reason for your answer)arrow_forwardCalculate the fraction of Asp that has its side chain protonated at pH 7.4arrow_forwardExplain the order of elution (with a buffer of pH 4) of the following pairs of amino acids through a column packed with Dowex 50a. aspartate before serine c. valine before leucineb. serine before alanine d. tyrosine before phenylalaninearrow_forward
- Explain how you would synthesise the dipeptide, H-Glu-Phe-OH, starting from the constituent amino acids? Explain all steps that are necessary and discuss a mechanism for a C-terminus protection and the coupling reaction.arrow_forwardGive typed solution only Which of the amino acids shown represents the structure of Ile at pH 10?arrow_forwardUsing the information below, determine the amino acid sequence of the peptide, and explain how your structure is consistent with each piece of information. Complete hydrolysis by 6 M HCl at 110°C followed by amino acid analysis indicated the presence of Gly, Leu, Phe, and Tyr in a 2:1:1:1 molar ratio. Treatment of the peptide with1-fluoro-2,4-dinitrobenzene followed by complete hydrolysis and chromatography indicated the presence of the 2,4-dinitrophenyl derivative of tyrosine. No free tyrosine could be found. Complete digestion of he peptide with pepsin (which cleaves on the amino side of aromatic residues) followed by chromatography yielded a dipeptide containing Phe and Leu and a tripeptide containing Tyr and Gly in a 1:2 ratio.arrow_forward
- The isoelectric point of tyrosine is 5.63. Toward which electrode does tyrosine migrate on paper electrophoresis at pH 7.0?arrow_forwardBradykinin is a linear nonapeptide released by blood plasma globulin in response to a wasp sting. It is a very potent pain-causing agent. Its constituent amino acids are 2R, G, 2F, 3P, S. The sue of 2,4-dinitrofluorobenzene and carboxypeptidase shows that both terminal residues are arginine. Partial hydrolysis of bradykinin gives the following di- and tripeptides: FS + PGF +PP + SPF + FR + RParrow_forwardWhat is the fraction of EDTA at pH 10.7?arrow_forward
- The pka of ascorbic acid is 4.2 at 24C. At what pH is the ratio of the deprotonated form to the protonated form 10:1? Prove using the Henderson-Hasselback equation. How to resolve this.arrow_forwardWhy is glycine the only amino acid commonly observed in peptides inconformations with phi and psi values which are not restricted to the values in the Ramachandran plot below?arrow_forwardTyrosine is an amino acid whose side chain has a pKa of 10.1. At pH 7, what protonation form would you expect to find it in?arrow_forward
- Organic ChemistryChemistryISBN:9781305580350Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. FootePublisher:Cengage LearningIntroduction to General, Organic and BiochemistryChemistryISBN:9781285869759Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar TorresPublisher:Cengage Learning