Interpretation:
As the isoelectric point of Histidine is 7.64, on paper electrophoresis towards which electrode does it migrate at
Concept Introduction:
The isoelectric point of an amino acid is the pH at which the molecules in solution have net charge of zero.
The paper electrophoresis is the technique which is used for the separation of amino acids. In this technique, a paper strip coated with a buffer whose pH is predetermined is placed between the two electrodes. When the sample is placed and the electrical potential is applied, the positively charged amino acids moves towards the cathode (negative electrode) and the negatively charged amino acids moves towards the anode (positive electrode). After separation, the paper strip is dried and sprayed with the dye to make the separated amino acids visible.
Want to see the full answer?
Check out a sample textbook solutionChapter 27 Solutions
Organic Chemistry
- What are the different technical considerations in performing the serum protein electrophoresis method? What should be done to obtain acceptable performance?.arrow_forwardBriefly explain how anodic stripping analysis is able to be used for quantifying DNAarrow_forwardIf you had a mixture of proteins with different sizes, shapes, and charged and you separated them with electrophoresis, which proteins would move fastest toward anode (positive electrode)?arrow_forward
- Write with the reasons why a separation will occur when a mixture of polypeptides with isoelectric points 4,3, 8,7 and 9,2 is applied to a column containing an anion-exchanging filler.arrow_forwardWhat is the fraction of EDTA at pH 10.7?arrow_forwardExplain the general mechanism of electron ionization (analyte requirements, ion source, energylevels, etc.)arrow_forward
- A protein with a pI of 7.5 will be purified using ion-exchange chromatography. Which ion exchange resin is suitable to use if the buffer has a pH of 7.0?a. A resin with a quaternary amino groupb. A resin with sulfonic acid groupc. A resin containing primary aromatic/aliphatic amino groupd. A resin that has carboxylic acid grouparrow_forwardWhy does a molecularly imprinted polymer selectively bind a desired analyte?arrow_forwardDefine the following terms :(i) Sorption(ii) Tyndall effect(iii) Electrophoresisarrow_forward
- What are the basic medium, acid medium, and zwitterion formulas of phenylalanine?arrow_forwardOptimum cell concertation is very important for successful bone marrow culture. A technologist received 2.7 ml bone marrow specimen with concentration 20 x 10^6 cell/ml, she add 1ml this bone marrow to 9 ml Marrow-Max culture medium to set up a bone marrow culture. What is final cell concentration in this bone marrow culture?arrow_forwardWhich are true for polarography? I Polarography is amperometry conducted with a dropping Hg electrode. II The diffusion current in the plateau region of the polarographic wave is proportional to the concentration of the analyte and is used for quantitative analysis. III The half-wave potential for maximum current is characteristic of a given analyte in a given medium and can be used for qualitative analysis for the analyte. IV Fresh Hg drops give reproducible results. V Polarography is used to primarily to study oxidations Hg may reduce when studying reductions. A.I, III, and IV B. II, III, and V C. I and V D. III, IV, and V E. II, III, and IVarrow_forward
- Principles of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage LearningOrganic ChemistryChemistryISBN:9781305580350Author:William H. Brown, Brent L. Iverson, Eric Anslyn, Christopher S. FootePublisher:Cengage Learning