ORGANIC CHEMISTRY-EBOOK>I<
9th Edition
ISBN: 9781305084414
Author: McMurry
Publisher: INTER CENG
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Chapter 28.SE, Problem 36AP
Show the steps involved in a laboratory synthesis of the DNA fragment with the sequence CTAG.
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DNA Replication1) Construct the complementary strand of DNA (strand II) that would pair with the DNA strand I sequence below. Remember that nucleotides are always added in the 5 prime to 3 prime direction. The base pairing rules are:
DNA DNAadenine (A) always bonds with thymine (T) thymine (T) always bonds with adenine (A) guanine (G) always bonds with cytosine (C) cytosine (C) always bonds with guanine (G)
STRAND I: 3’ T A C T A T A G T C T G T C T C C C A C T 5’
STRAND II: 5’ 3’
During base excision repair, DNA glycosylase cleaves theN-glycosidic link between the altered base and the deoxyribose component of the nucleotide. Draw a typical nucleotideand indicate which bond is cleaved.
Explain the basic principles and chemistry of DNA sequencing by Sanger’s Dideoxy-Chain-Termination Method.
Chapter 28 Solutions
ORGANIC CHEMISTRY-EBOOK>I<
Ch. 28.1 - Draw the full structure of the DNA dinucleotide...Ch. 28.1 - Draw the full structure of the RNA dinucleotide...Ch. 28.2 - What sequence of bases on one strand of DNA is...Ch. 28.4 - Show how uracil can form strong hydrogen bonds to...Ch. 28.4 - What RNA base sequence is complementary to the...Ch. 28.4 - From what DNA base sequence was the following RNA...Ch. 28.5 - List codon sequences for the following amino...Ch. 28.5 - List anticodon sequences on the tRNAs carrying the...Ch. 28.5 - What amino acid sequence is coded by the following...Ch. 28.5 - What is the base sequence in the original DNA...
Ch. 28.7 - Prob. 11PCh. 28.7 - Prob. 12PCh. 28.SE - Identify the following bases, and tell whether...Ch. 28.SE - Identify the following nucleotide, and tell how it...Ch. 28.SE - Amine bases in nucleic acids can react with...Ch. 28.SE - The final step in DNA synthesis is deprotection by...Ch. 28.SE - The final step in the metabolic degradation of...Ch. 28.SE - One of the steps in the biosynthesis of a...Ch. 28.SE - One of the steps in the metabolic degradation of...Ch. 28.SE - One of the steps in the biosynthesis of uridine...Ch. 28.SE - Human brain natriuretic peptide (BNP) is a small...Ch. 28.SE - Human and horse insulin both have two polypeptide...Ch. 28.SE - The DNA of sea urchins contains about 32% A. What...Ch. 28.SE - The codon UAA stops protein synthesis. Why does...Ch. 28.SE - Which of the following base sequences would most...Ch. 28.SE - For what amino acids do the following...Ch. 28.SE - Prob. 27APCh. 28.SE - Prob. 28APCh. 28.SE - Draw the complete structure of the ribonucleotide...Ch. 28.SE - Draw the complete structure of the...Ch. 28.SE - Give an mRNA sequence that will code for the...Ch. 28.SE - Give an mRNA sequence that will code for the...Ch. 28.SE - What amino acid sequence is coded for by the...Ch. 28.SE - What amino acid sequence is coded for by the...Ch. 28.SE - Prob. 35APCh. 28.SE - Show the steps involved in a laboratory synthesis...Ch. 28.SE - Draw the structure of cyclic adenosine...Ch. 28.SE - Prob. 38AP
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- The DNA of bacteriophage lambda has 1.2 x 10^5 nucleotides. How many proteins of molecular weight 40,000 could be coded by this DNA? Assume a molecular weight of 100 for the average amino acid.arrow_forward7. Consider the DNA segment 5'-ATGACGGTTTACTAAGCC-3' a. Write the mRNA sequence that would be obtained from the segment's transcription.arrow_forwardSection one. Match enzyme or molecule with its function in bacteria. Answers may be used more than once or not at all. DNA ligase d. tRNA promoter а. g. b. DNA helicase 1RNA h. terminator е. RNA polymerase f. DNA polymerase III i. stop codon с. 37. main enzyme in catalyzing DNA → mRNA transcription 38. unwinds double-stranded DNA at the replication fork 39. Where transcription stops 40. adds new DNA bases in the 5'→3’ direction 41. the "translator" molecule 42. contains codons 43. contains anti-codonsarrow_forward
- Consider the following DNA fragment. Identify the 5' and 3' ends, and label each of the nitrogenous basesarrow_forwardOne strand of DNA has the base sequence: C G A T T G G C A G T C A T. Determine the sequence of bases in the complementary strand of mRNA that would form next to this DNA strand.arrow_forwardDraw the complete structure of the ribonucleotide codon UAC. For what amino acid does this sequence code?arrow_forward
- What would be the ramifications if DNA were single stranded?arrow_forwardThe DNA template strand segment 3TTCAAACCGTAC5 upon transcription and translation produces the amino acid sequence Lys-Phe-Gly-Met. What is the amino acid sequence produced if a frameshift mutation occurs in which the third A base in the original DNA sequence is removed?arrow_forward
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Nucleic acids - DNA and RNA structure; Author: MEDSimplified;https://www.youtube.com/watch?v=0lZRAShqft0;License: Standard YouTube License, CC-BY