Prescott's Microbiology
11th Edition
ISBN: 9781260409062
Author: WILLEY, Joanne
Publisher: MCGRAW-HILL HIGHER EDUCATION
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Chapter 29.1, Problem 1MI
What happens to the flow stream in fluorescence activated cell sorting (FACS)?
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Chapter 29 Solutions
Prescott's Microbiology
Ch. 29.1 - What happens to the flow stream in fluorescence...Ch. 29.1 - Prob. 1CCCh. 29.1 - Prob. 2CCCh. 29.1 - Describe the rationale upon which the extinction...Ch. 29.1 - Prob. 4CCCh. 29.2 - Prob. 1MICh. 29.2 - Prob. 1CCCh. 29.2 - Prob. 2CCCh. 29.2 - Prob. 3CCCh. 29.2 - Prob. 4CC
Ch. 29.3 - How do radioactive and stable isotopes differ?...Ch. 29.3 - Prob. 2CCCh. 29.3 - Prob. 3CCCh. 29.3 - Prob. 4CCCh. 29.3 - What is the difference between MAR and MAR-FISH?...Ch. 29 - Prob. 1RCCh. 29 - Prob. 2RCCh. 29 - Prob. 3RCCh. 29 - Prob. 4RCCh. 29 - Prob. 5RCCh. 29 - Prob. 6RCCh. 29 - Prob. 7RCCh. 29 - Prob. 8RCCh. 29 - Prob. 9RCCh. 29 - Prob. 10RCCh. 29 - Prob. 11RCCh. 29 - How might you attempt to grow in the laboratory a...Ch. 29 - Both acetate and CO2 can be used by methanogenic...Ch. 29 - Prob. 3AL
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- 5) Using FRAP (Fluorescence Recovery After Photobleaching), you can measure the diffusion rate of membrane proteins. You attach a fluorescent marker to your protein of interest, bleach a small region of the cell membrane with an intense laser light, and determine the time it takes for the bleached spot to recover fluorescent signal (see figure below). (a) (b) Bleach Laser bleaching of fluorescent marker Fluorescence intensity recovery Recovery Time How would the recovery time of your protein change if the membrane contained a higher concentration of unsaturated fatty acids? Why? How would the recovery time of your protein change if you conducted the experiment at a lower temperature? Why? How would the recovery time of your protein change if it were anchored to the membrane skeleton? Why?arrow_forwardWhat role does chromatin structure play in cell memory and in cell reprogramming?arrow_forwardWhat is one manner in which ERAD may identify misfolded proteins? Why is dislocation of these misfolded proteins to the cytoplasm necessary?arrow_forward
- What is differential centrifugation? Explain how it can be used to separate cellular organelles.arrow_forwardWhat could be an example of in vitro experiment to identify cytoskeleton accessory proteins?arrow_forwardFluorescence-activated cell sorting (FACS) is a powerful technique for separating cells according to their content of particular molecules. For example, a fluorescence-labeled antibody specific for a cell-surface protein can be used to detect cells containing such a molecule. Suppose that you want to isolate cells that possess a receptor enabling them to detect bacterial degradation products. However, you do not yet have an antibody directed against this receptor. Which fluorescence-labeled molecule would you prepare to identify such cells?arrow_forward
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