Concept explainers
(a)
To identify: The restriction enzymes that produce blunt ends.
Concept introduction: Restriction enzymes recognize their specific target sequence and excise deoxyribonucleic acid (DNA) at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. These make staggered cuts at or adjacent to their specific recognition site and most of them produce sticky ends.
(b)
To identify: The restriction enzymes that recognize and cleave the same sequence.
Concept introduction: Restriction enzymes recognize their specific target sequence and excise deoxyribonucleic acid (DNA) at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. These make staggered cuts at or adjacent to their specific recognition site and most of them produce sticky ends.
(c)
To identify: The restriction enzymes that produce identical sticky ends.
Concept introduction: Restriction enzymes recognize their specific target sequence and excise deoxyribonucleic acid (DNA) at that sequence or adjacent to it. These specific sequences are known as restriction sites and the enzymes are known as DNA cutting enzymes. These make staggered cuts at or adjacent to their specific recognition site and most of them produce sticky ends.
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Chapter 3 Solutions
FUNDAMENTALS OF BIOCHEMISTRY WPNG 1-SEME
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