MICROBIOLOGY-ACCESS >CUSTOM<
13th Edition
ISBN: 9780135668825
Author: Tortora
Publisher: PEARSON
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Chapter 3, Problem 9R
Fill in the following table regarding the
Appearance After This Step | |||
Steps | Gram-Positive Cells | Gram-Negative Cells | |
Crystal violet | a. __________ | e. __________ | |
Iodine | b. __________ | f. __________ | |
Alcohol-acetone | c. __________ | q. __________ | |
Safranin | d. __________ | h. __________ |
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Students have asked these similar questions
Explain how bacterial cells would look in the gram staining procedure if the following mistakes were made:
a. Decolorizer left on too long
b. Decolorizer not left on long enough
c. Slide not heat-fixed before staining
Which of the following describes the usefulness of a Gram stain. Select all that apply.
a. A Gram stain can be used to check for bacteria at the site of a suspected infection or in certain body fluids, such as blood or urine.
b. A Gram stain can be used to determine whether penicillin will be effective at treating the patient or whether a different antibiotic should be used.
c. A Gram stain can determine whether the bacteria have a thick or thin layer of peptidoglycan in their cell wall.
Explain the principle of Gram staining. (You do not need to describe the procedure step by step).
Chapter 3 Solutions
MICROBIOLOGY-ACCESS >CUSTOM<
Ch. 3 - Fill in the following blanks. 1. 1 m = ________ m...Ch. 3 - Prob. 2RCh. 3 - Prob. 3RCh. 3 - Prob. 4RCh. 3 - Prob. 5RCh. 3 - Why is a mordant used in the Gram stain? In the...Ch. 3 - Prob. 7RCh. 3 - Prob. 8RCh. 3 - Fill in the following table regarding the Gram...Ch. 3 - NAME IT A sputum sample from Calle, a 30-year-old...
Ch. 3 - Through the microscope, the green structures are...Ch. 3 - Prob. 2MCQCh. 3 - Carbolfuchsin can be used as a simple stain and a...Ch. 3 - Prob. 4MCQCh. 3 - Which of the following is not a functionally...Ch. 3 - Which of the following pairs is mismatched? 1....Ch. 3 - Assume you stain Clostridium by applying a basic...Ch. 3 - Prob. 8MCQCh. 3 - In 1996, scientists described a new tapeworm...Ch. 3 - Prob. 10MCQCh. 3 - Prob. 1ACh. 3 - Prob. 2ACh. 3 - Why isnt the Gram stain used on acid-fast...Ch. 3 - Endospores can be seen as refractile structures in...Ch. 3 - In 1882, German bacteriologist Paul Erhlich...Ch. 3 - Laboratory diagnosis of Neisseria gonorrhoeae...Ch. 3 - Assume that you are viewing a Gram-stained sample...
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- Crystal violet ( choose all that apply) A. Is not a good differential stain B. Will stain the cytoplasm of the cell walls C. Can be used to stain peptidoglycan on bacteria cell wall D. Can be used as an effective antimicrobialarrow_forwardBoth crystal violet and safranin are basic stains and may be used to do simple stains on Gram positive and negative cells. This being the case, explain how they end up staining Gram positive and gram negative cells differently.arrow_forwardExamine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information: A. Shape....... gram stain( +) , Gram (-) or both B. Stained color under microscope C. PG Wall thick, thin or botharrow_forward
- Explain the difference between Gram positive and Gram negative cell walls. Describe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?) How is the Gram stain used clinically to help identify the cause of an infection?arrow_forwardExamine the image below. The bacteria in this image have been treated with gram straining procedure. Indicate the following information A. Shape ........; Gram stain ( Gram (+), Gram (-) or both ... B. Stained color under microscope..... . C. Is the PG wall think, thin or both ..arrow_forwardWhy is it necessary to use a 24-hrold culture in gram staining? What does Gram staining reveal owing to its use in clinical diagnosis? Indicate color staining results of gram positive and negative bacteria for every hypothetical scenario. Modification in Procedure Staining results 1. Crystal violet was replaced with methylene blue. G+ G- 2. Mordant was skipped G+ G- 3. Decolorizing step was omitted G+ G- 4. counterstain was not used? G+ G- 5. Crystal violet and safranin was swapped in position. G+ G- What bacterial specimen represent the following: (a) positive simple staining; and (b) negative simple staining viewed under OIO. Provide details such as specimen and bacterial stain used and reference. Positive Staining 1000 X Negative Staining 1000 Xarrow_forward
- Describe the procedure for performing a Gram stain. What is the procedure? How does each component work? (ie. What does the mordant do?)arrow_forwardExplain why only gram-negative cells undergo decolorization during the gram staining procedure. Cite the purpose of each of the following reagents in a differential staining procedure. Primary stain Mordant Decolorizing Agent Counter stain What might happen if the Gram staining procedure is performed on a culture incubated for a little over a dayarrow_forwardBased from the results obtained from Gram staining, explain why bacterial cells retained or changed their color. Briefly explain the principle behind the procedure. Cite advantages of differential staining method (Gram staining and endospore staining) over simple staining only. List other staining methods aside from those you performed in the laboratory. conclusion:arrow_forward
- What is the function of the mordant used in the Gram staining procedure? If the iodine step is skipped, what color are Gram-negative cells observedarrow_forwardThe above photograph (IMAGE ATTACHED) of a cheek smear was obtained using the high-power objective of a compound microscope. What is the size of the circled cheek cell in millimeters AND nanometers?arrow_forwardBacillus subtilis (Gram-positive) and Pseudomonas aeroginosa (Gram-negative) bacteria are heat-fixed on slide and subjected to Gram staining. What do you observe under the microscope when the following steps are skipped? Please explain.a. Staining with safraninb. Staining with Gram iodinec. Decolorizationarrow_forward
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