(a) Interpretation: The autoradiographic pattern for strands (i) to (v) should be determined. Concept introduction: DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA. RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

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Answer 1

Question

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

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gene. If the JM109 strain is transformed by the PBKSK plasmid, the strain will produce the B-galactosidase (from the lac gene) and will hydrolyze X-gal to produce the blue compound. Therefore, colonies that were transformed and contain the pBSKS wil you appear blue. IPTG & X-Gal & NO colonies Amp E. coli JM109 E. coli JM109 50 mM calcium chloride-15% glycerol lac lac lac IPTG & I Recovery X-Gal solution at -702C PBSKS White colonies E. coli JM109 E. coli JM109 ampR amp I amp lac lac Heat Shock Non-transformed 42°C E. coli JM109 E. coli JM109 amps amps lac lac IPTG & X-Gal lac I Recovery lac PBSKS BLUE colonies PBSKS ampRI (amp Transformed IPTG & X-Gal & BLUE colonies Amp Hypotheses: Circle the correct answer 1. If PBSKS is transformed into JM109 cells, colonies will be (able/not able) to grow in the presence of ampicillin. a. Why? _ 2. If PBSKS is transformed into JM109 cells, colonies in media with IPTG (will/will not) induce the production the B- galactosidase enzyme. a. Why?_ 3. If…

Answer 2

Question

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

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Answer 3

Question

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

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Answer 4

Question

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

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Answer 5

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 6

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 7

Chapter 30, Problem 12P

Interpretation Introduction

(a)

Interpretation:

The autoradiographic pattern for strands (i) to (v) should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 8

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 9

Interpretation Introduction

(b)

Interpretation:

The possible effect of rifampicin on the RNA synthesis in the given system should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 10

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 11

Interpretation Introduction

(c)

Interpretation:

The reason for the difference between the heparin blocks the elongation of RNA primer before the transcription but not after should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 12

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 13

Interpretation Introduction

(d)

Interpretation:

The length of the longest product in the absence of four ribo-nucleoside triphosphate should be determined.

Concept introduction:

DNA synthesis can be defined as the process by which the deoxynucleic acids such as adenine, thymine, cytosine, and guanine are associated together to form DNA.

RNA synthesis (transcription) is the synthesis of an RNA molecule from the nucleotide’s adenine, cytosine, guanine, or uracil. The nucleotides are combined together by the enzyme RNA Polymerase.

Answer 14

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Answer 15

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Answer 17

Ch. 30 - Prob. 1P Ch. 30 - Prob. 2P Ch. 30 - Prob. 3P Ch. 30 - Prob. 4P Ch. 30 - Prob. 5P Ch. 30 - Prob. 6P Ch. 30 - Prob. 7P Ch. 30 - Prob. 8P Ch. 30 - Prob. 9P Ch. 30 - Prob. 10P

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