EBK LIFE: THE SCIENCE OF BIOLOGY
EBK LIFE: THE SCIENCE OF BIOLOGY
11th Edition
ISBN: 8220103935432
Author: Sadava
Publisher: MAC HIGHER
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Chapter 35.1, Problem 1R
Summary Introduction

To review:

The reason behind the discovery of some essential nutrients for plant growth century and a half later from when the first experiments to determine essential elements was discovered.

Introduction:

Plants need inorganic elements for growth. These inorganic elements can either be required in high quantities (macronutrients) or in trace amounts (micronutrients). They are ‘essential’ because, without them, plant will not survive.

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What is a nutrient solution? Why is it more convenient to use liquid medium, as opposed to soil, to grow plants in nutritional studies?
The following four chemicals commonly found in defined growth media contain all of the necessary atomic macronutrients, namely C, O, N, H, S and P, which microbes require:  glucose (C6H12O6), K2HPO4, MgSO4.7H2O, and (NH4)HPO4.   Part a.  Create a simple diagram to map which growth media chemical contributes atoms to which of the four biological macromolecules (amino acids, polysaccharides, lipids, and ribonucleic acids).   Part b.  Consider a cell culture broth that originally (before inoculation with cells) contains 5.0 gr/L glucose (C6H12O6), 1.2 gr/L K2HPO4, 0.3 gr/L MgSO4∙7H2O, and 1.0 gr/L (NH4)HPO4.  Create a table showing how much of each of the different atoms (C, H, O, K, P, Mg, S, N) are available for incorporation into cell macromolecules (assume 50% of the glucose is catabolized, while the remainder goes for energy (respiration).  Express your answers with units of grams of atomic species / liter of culture broth.
To be essential, an element must be acting within the plant, not outside it. What does this mean? Discuss the roles of iron and phosphorus in determining which elements are essential for a plant.
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