Foundations in Microbiology
10th Edition
ISBN: 9781259705212
Author: Kathleen Park Talaro, Barry Chess Instructor
Publisher: McGraw-Hill Education
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Chapter 3.L1, Problem 12MCQ
Summary Introduction
Introduction:
Specimens are pretreated with dyes to increase contrast and thereby facilitate better visibility. Staining is a procedure that involves the application of color or dyes to specimens. Dyes impart a color to cells or cell parts by becoming affixed to them through a
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Bacteria tend to stain more readily with cationic (positively charged) dyes because bacterial surfaces a. contain large amounts of alkaline substances. b. contain large amounts of acidic substances. c. are neutral. d. have thick cell walls.
Bacteria tend to stain more readily with cationic (positively charged) dyesbecause bacteriaa. contain large amounts of alkaline substances on their surfaces.b. contain large amounts of acidic substances on their surfaces.c. carry a neutral charge on their surfaces. d. have thick cell walls.
The use of the Gram stain in microbiology is important because it differentiates
A) Bacteria from Archaea.
B) prokaryotic from eukaryotic cells.
C) bacterial cells with different types of cell walls.
D) archaeal cells with different types of metabolism.
Chapter 3 Solutions
Foundations in Microbiology
Ch. 3.1 - Explain what unique characteristics of...Ch. 3.1 - Briefly outline the processes and purposes of the...Ch. 3.1 - Name the notable features of microorganisms that...Ch. 3.1 - In one sentence each, define what is involved in...Ch. 3.2 - Prob. 3ELOCh. 3.2 - Prob. 4ELOCh. 3.2 - Prob. 5ELOCh. 3.2 - Prob. 6ELOCh. 3.2 - Prob. 7ELOCh. 3.2 - Prob. 8ELO
Ch. 3.2 - Prob. 3CYPCh. 3.2 - Prob. 4CYPCh. 3.2 - Prob. 5CYPCh. 3.2 - Prob. 6CYPCh. 3.2 - Prob. 7CYPCh. 3.2 - Prob. 8CYPCh. 3.2 - Prob. 9CYPCh. 3.2 - Compare the way that the image is formed in the...Ch. 3.3 - Prob. 9ELOCh. 3.3 - Define dyes and describe the basic chemistry...Ch. 3.3 - Prob. 11ELOCh. 3.3 - Distinguish between simple, differential, and...Ch. 3.3 - Describe the process of Gram staining and how its...Ch. 3.3 - Prob. 11CYPCh. 3.3 - Explain what happens in positive staining to cause...Ch. 3.3 - Prob. 13CYPCh. 3.3 - For a stain to be considered differential, what...Ch. 3.3 - Prob. 15CYPCh. 3.4 - Prob. 14ELOCh. 3.4 - Prob. 15ELOCh. 3.4 - Explain what an isolated colony is and indicate...Ch. 3.4 - Differentiate between a pure culture, subculture,...Ch. 3.4 - What kinds of data are collected during...Ch. 3.4 - Prob. 19ELOCh. 3.4 - Prob. 16CYPCh. 3.4 - Prob. 17CYPCh. 3.4 - Prob. 18CYPCh. 3.4 - Compare and contrast three common laboratory...Ch. 3.4 - Describe how an isolated colony forms.Ch. 3.4 - Prob. 21CYPCh. 3.5 - Prob. 20ELOCh. 3.5 - Name the three general categories of media, based...Ch. 3.5 - Compare and contrast liquid, solid, and semisolid...Ch. 3.5 - Prob. 23ELOCh. 3.5 - Prob. 24ELOCh. 3.5 - Identify the qualities of enriched, selective, and...Ch. 3.5 - Explain what it means to say that microorganisms...Ch. 3.5 - Describe live media and the circumstances that...Ch. 3.5 - Describe the main purposes of media, and compare...Ch. 3.5 - Differentiate among the ingredients and functions...Ch. 3.5 - Explain the two principal functions of dyes in...Ch. 3.5 - Why are some bacteria difficult to grow in the...Ch. 3.5 - What conditions are necessary to cultivate viruses...Ch. 3.L1 - Prob. 1MCQCh. 3.L1 - Prob. 2MCQCh. 3.L1 - Prob. 3MCQCh. 3.L1 - Prob. 4MCQCh. 3.L1 - Prob. 5MCQCh. 3.L1 - Prob. 6MCQCh. 3.L1 - Resolution is _____________ with a longer...Ch. 3.L1 - Prob. 8MCQCh. 3.L1 - Prob. 9MCQCh. 3.L1 - The specimen for an electron microscope is always...Ch. 3.L1 - Motility is best observed with a a. hanging drop...Ch. 3.L1 - Prob. 12MCQCh. 3.L1 - Prob. 13MCQCh. 3.L1 - Prob. 14MCQCh. 3.L1 - What type of medium is used to maintain and...Ch. 3.L1 - Prob. 16MCQCh. 3.L1 - Multiple Matching. For each type of medium, select...Ch. 3.L1 - Prob. 1CSRCh. 3.L1 - Prob. 2CSRCh. 3.L1 - Prob. 3CSRCh. 3.L1 - Prob. 1WCCh. 3.L1 - Prob. 2WCCh. 3.L1 - Prob. 3WCCh. 3.L1 - Describe the steps of the Gram stain, and explain...Ch. 3.L1 - Describe the steps you would take to isolate,...Ch. 3.L1 - Prob. 6WCCh. 3.L1 - Evaluate the following preparations in terms of...Ch. 3.L2 - A certain medium has the following composition: a....Ch. 3.L2 - a. Name four categories that blood agar fits. b....Ch. 3.L2 - Prob. 3CTCh. 3.L2 - Go back to section 1.2 and observe the six...Ch. 3.L2 - Since the dyes for the Gram stain are not specific...Ch. 3.L2 - Examine figure 3.10a, b (shown here). If you...Ch. 3.L2 - Prob. 2VC
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- Why are staining methods used in microbiology? Choose all that apply A. Staining results can have medical implications for an infected B. To clearly see the internal structures of the bacteria C. The stains can be used to dinginuish between bacteria type D. It is easier to very small microscope.arrow_forwardDyes are used to stain specimens that will be observed using the microscope to a. increase the magnification. b. increase resolution. c. increase contrast. d. make it visible. e. none of the above.arrow_forwardWhich of the following is NOT characteristic of the glycocalyx found in bacteria? Select one: A) a structure that can be visualized using an acidic negative stain and a basic counterstain B) creates a slimy, slippery coating that prevents bacteria from attaching to surfaces C) if firmly attached, contributes to bacterial virulence D) a viscous coating surrounding the cell made of polysaccharide, polypeptide, or botharrow_forward
- Select a statement that is not true about bacteria A. Some bacteria cause human disease B. Some bacteria are autotrophs C. Bacteria can be found within rocks kilometers below the Earth’s surface D. All organisms with cells containing a membrane-enclosed nucleus and organelles are eukaryotes, whereas all organisms with cells lacking a membrane-enclosed nucleus and organelles are bacteriaarrow_forwardBacteria are microscopic single-celled organisms that are far too small to detect with the naked eye. Describe how you could grow enough bacteria in a colony to be viewed without a microscope. What is the relationship between the cells that form a colony?arrow_forwardProkaryotes stain as Gram-positive or Gram-negative because of differences in the_______. a. cell wall b. cytoplasm c. nucleus d. chromosomearrow_forward
- Polymyxins are antibiotics that are used in the treatment of Gram-negative bacterial infections. You are given samples of two different types of bacteria. You are told that Bacteria A is Gram-negative bacteria and Bacteria B is Gram-positive bacteria. Describe, in your own words, how you would confirm the bacterial types.arrow_forwarda) What is morphology? b) Describe the common morphological characteristics of bacteria that are often used in microbiological studies. c) Explain the chemical and physical requirements needed for bacterial growth d) Explain the importance of using growth medium and devise a STEP-BY-STEP plan to grow bacterial cell cultures in the laboratory.arrow_forwardWhat kind of microscope was invented in 1932 that was able to record images of transparent specimens? a. compound microscope b. PCM c. TEM d. SEMarrow_forward
- Which of the following is NOT an optical microscope? a. dark-field c. atomic force b. confocal d. fluorescentarrow_forwardUse the following diagram to answers the questions. A. Is this cell Gram + or Gram -? Provide 2 pieces of evidence to support your choice. B. If you are infected by this bacterium can the antibiotic be effective in killing it. Explain. C. NAME and label the structures in the diagram that could be involved in conjugation? Why conjugation is an important process for the bacteriaarrow_forwardA scientist develops a new medication that is a protein compound and must be administered by injection. Which of the following would be the most effective and safest means of preparing a nearly sterile solution of the new medication? Select one: a. ultraviolet irradiation b. autoclaving c. filtration d. Lyophilization e dilution with alcoholarrow_forward
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Prokaryotic vs. Eukaryotic Cells (Updated); Author: Amoeba Sisters;https://www.youtube.com/watch?v=Pxujitlv8wc;License: Standard youtube license