EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Textbook Question
Chapter 4, Problem 2TMW
Why is a Gram-negative bacterium colorless but a Gram-positive bacterium purple after it is rinsed with decolorizer?
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You are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point?
○ pink
○ colorless
○ blue
○ purple
Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
Cite the purpose of each of the following reagents in a differential staining procedure.
Primary stain
Mordant
Decolorizing Agent
Counter stain
What might happen if the Gram staining procedure is performed on a culture incubated for a little over a day
Chapter 4 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 4 - Prob. 1TMWCh. 4 - Why is a Gram-negative bacterium colorless but a...Ch. 4 - Why didnt Linnaeus create taxonomic groups for...Ch. 4 - Necrotizing Fasciitis Fever, chills, nausea,...Ch. 4 - Why is magnification high and color absent in an...Ch. 4 - Prob. 1MCCh. 4 - Prob. 2MCCh. 4 - Prob. 3MCCh. 4 - Curved glass lenses _________ light. a. refract b....Ch. 4 - Prob. 5MC
Ch. 4 - Prob. 6MCCh. 4 - Prob. 7MCCh. 4 - Prob. 8MCCh. 4 - Prob. 9MCCh. 4 - In the binomial system at nomenclature, which term...Ch. 4 - Prob. 1FIBCh. 4 - Prob. 2FIBCh. 4 - Prob. 3FIBCh. 4 - Fill in the Blanks 4. ___________ refers to...Ch. 4 - Fill in the Blanks 5. Cationic chromophores such...Ch. 4 - Prob. 1VICh. 4 - Label the microscope.Ch. 4 - Prob. 1SACh. 4 - Critique the following definition of magnification...Ch. 4 - Prob. 3SACh. 4 - Put the following substances in the order they are...Ch. 4 - Prob. 5SACh. 4 - Prob. 6SACh. 4 - Prob. 7SACh. 4 - Prob. 8SACh. 4 - Miki came home from microbiology lab with green...Ch. 4 - Why is the definition of species as successfully...Ch. 4 - With the exception of the discovery of new...Ch. 4 - Prob. 4CTCh. 4 - Prob. 5CTCh. 4 - In what ways are the Gram stain and the acid-fast...Ch. 4 - Microbiologists have announced the discovery of...Ch. 4 - Why is the genus name Coccus placed within...Ch. 4 - A clinician obtains a specimen of urine from a...Ch. 4 - Using the following terms, fill in the following...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- The acid-fast bacterium, Mycobacterium smegmatis, is relatively safe to be used by students in performing the acid-fast stain technique because of its thin cell wall. However, if this organism is used for Gram-stain, it can also take the Gram stain reaction. Why is this so?arrow_forwardWhat staining technique is used here? What is the primary stain, decolorizing agent, and counter stain of this technique? Do we heat fix in here? Describe the organism with the blue color. Describe the organism with the bright pink color. Name the genus of bacteria with bright pink.arrow_forwardThe bacterial cells in the image below were stained with a high concentration of carbolfuschin for an extended period of time (5 minutes) while the slide was heated. The slide was decolorized with a mixture of acid and alcohol. The slide was then counter-stained with methylene blue. What kind of stain does this describe?arrow_forward
- What is the difference between sterilization and disinfection? For lab applications which is preferred?arrow_forwardWhat color would the bacterial cells have when viewed microscopically after the following treatments: Gram Reaction Ammonium Oxalate Gram lodine Ethyl Alcohol Safranin Crystal Violet Gram-positive Gram-negativearrow_forwardThe acid fast bacterium, Mycobacterium smegmatis, is relatively safe for students to work with because of its thin cell wall. If a gram stain is to be performed on this particular microbe, it often takes the gram stain reaction. Why?arrow_forward
- Describe one error in the staining procedure that would result in a Gram-positive bacterium appearing pink.arrow_forwardExplain why some cells are pink and others are purple in Gram-stained bacterial smear.arrow_forwardAre there any chemical differences between the cell walls of gram-positive and negative bacteria that might explain differences in the rate of decolorization ? Does the age of a culture affect the gram stain reaction ? Why or why not?arrow_forward
- Endospores can be seen as refractile structures in unstained cells and as colorless areas in Gram-stained cells. Why is it necessary to do an endospore stain to verify the presence of endospores?arrow_forwardWhy is it important to follow sterile technique? What can happen to cause gram-positive bacteria to appear gram-negative?arrow_forwardImagine you accidentally applied water during the decolorization step instead of ethyl alcohol. Which of the following outcomes would you expect? Both Gram-positive and Gram-negative bacteria would appear reddish-pink Both Gram-positive and Gram-negative bacteria would appear purple Gram-positive bacteria would appear purple and Gram-negative bacteria reddish-pink Gram-positive bacteria would appear reddish-pink and Gram-negative bacteria purple Gram-positive bacteria would appear colorless and Gram-negative bacteria reddish-pinkarrow_forward
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