Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
12th Edition
ISBN: 9780134605203
Author: Ted R. Johnson, Christine L. Case
Publisher: PEARSON
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Textbook Question
Chapter 5, Problem 1CT
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH of the Stain affect the staining of bacteria?
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In staining, why is there a need for contrasting stains?
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Why are basic dyes more effective for bacterial staining than acidic dyes?
Chapter 5 Solutions
Laboratory Experiments in Microbiology (12th Edition) (What's New in Microbiology)
Ch. 5 - Of what value is a simple stain?Ch. 5 - What is the purpose of heat-fixing the smear?Ch. 5 - Another method of fixing smears is to use methanol...Ch. 5 - In heat-fixing, what would happen if too much heat...Ch. 5 - Methylene blue can be prepared as a basic stain or...Ch. 5 - Prob. 2CTCh. 5 - Bacteria can be seen without staining. Why, then,...Ch. 5 - Quality control staff in a sterilization unit of a...
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- What is the difference between simple and differential stains? Give an example of a differential stain.arrow_forwardWhat is the secondary stain in both acid-fast stain methods?In the Ziehl-Neelsen acid-fast stain, what is the mordant?arrow_forwardThe acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C. Gerrard-Lampard Stain D. Kinyoun methodarrow_forward
- The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C. Malachite green D. Safraninearrow_forwardFor each stain, indicate if it’s a cationic dye or an anionic dye (2 pts). Stain Cationic or Anionic Dye malachite green crystal violet nigrosin safraninarrow_forwardIn Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?arrow_forward
- What is the difference between Giemsa and Wright stain?arrow_forwardAbout the Gram staining technique, explain: What is the purpose of using lugol and alcohol in this type of coloring?arrow_forwardYou mistakenly confused the primary stain and counter stain with crystal violet, how does you misture now appear when viewed with oil immersion?arrow_forward
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