Biochemistry
9th Edition
ISBN: 9781305961135
Author: Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal
Publisher: Cengage Learning
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Chapter 5, Problem 23RE
REFLECT AND APPLY Design an experiment to purify protein X on an anion-exchange column. Protein X has an isoelectric point of 7.0.
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Biochemistry
Ch. 5 - RECALL What types of homogenization techniques are...Ch. 5 - RECALL When would you choose to use a...Ch. 5 - RECALL What is meant by salting out? How does it...Ch. 5 - RECALL What differences between proteins are...Ch. 5 - RECALL How could you isolate mitochondria from...Ch. 5 - RECALL Can you separate mitochondria from...Ch. 5 - RECALL Give an example of a scenario in which you...Ch. 5 - Prob. 8RECh. 5 - REFLECT AND APPLY You are purifying a protein for...Ch. 5 - Prob. 10RE
Ch. 5 - RECALL What is the basis for the separation of...Ch. 5 - RECALL What is the order of elution of proteins on...Ch. 5 - RECALL What are two ways that a compound can be...Ch. 5 - Prob. 14RECh. 5 - RECALL Why do most people elute bound proteins...Ch. 5 - RECALL What are two types of compounds that make...Ch. 5 - RECALL Draw an example of a compound that would...Ch. 5 - RECALL How can gel-filtration chromatography be...Ch. 5 - REFLECT AND APPLY Sephadex G-75 has an exclusion...Ch. 5 - Prob. 20RECh. 5 - RECALL What is the main difference between reverse...Ch. 5 - RECALL How does HPLC differ from ion-exchange...Ch. 5 - REFLECT AND APPLY Design an experiment to purify...Ch. 5 - REFLECT AND APPLY Referring to Question 23, how...Ch. 5 - Prob. 25RECh. 5 - REFLECT AND APPLY You wish to separate and purify...Ch. 5 - REFLECT AND APPLY An amino acid mixture consisting...Ch. 5 - REFLECT AND APPLY An amino acid mixture consisting...Ch. 5 - REFLECT AND APPLY In reverse-phase HPLC, the...Ch. 5 - REFLECT AND APPLY Gel-filtration chromatography is...Ch. 5 - RECALL What physical parameters of a protein...Ch. 5 - RECALL What types of compounds make up the gels...Ch. 5 - RECALL Of the two principal polymers used in...Ch. 5 - RECALL What types of macromolecules are usually...Ch. 5 - RECALL If you had a mixture of proteins with...Ch. 5 - RECALL What does SDSPAGE stand for? What is the...Ch. 5 - RECALL How does the addition of sodium...Ch. 5 - RECALL Why is the order of separation based on...Ch. 5 - RECALL The accompanying figure is from an...Ch. 5 - Prob. 40RECh. 5 - Prob. 41RECh. 5 - Prob. 42RECh. 5 - Prob. 43RECh. 5 - REFLECT AND APPLY What would happen during an...Ch. 5 - REFLECT AND APPLY A sample of an unknown peptide...Ch. 5 - REFLECT AND APPLY A sample of a peptide of unknown...Ch. 5 - REFLECT AND APPLY You are in the process of...Ch. 5 - REFLECT AND APPLY You are in the process of...Ch. 5 - Prob. 49RECh. 5 - Prob. 50RECh. 5 - Prob. 51RECh. 5 - Prob. 52RECh. 5 - Prob. 53RECh. 5 - Prob. 54RECh. 5 - RECALL What is the basis for the technique called...Ch. 5 - Prob. 56RECh. 5 - Prob. 57RECh. 5 - RECALL What are the main procedures involved in a...Ch. 5 - RECALL Where did western blot get its name?Ch. 5 - Prob. 60RECh. 5 - Prob. 61RECh. 5 - Prob. 62RECh. 5 - Prob. 63RECh. 5 - Prob. 64RECh. 5 - Prob. 65RE
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- REFLECT AND APPLY Suggest a reason why the same protein system moves both sodium and potassium ions into and out of the cell.arrow_forwardREFLECT AND APPLY It is frequently said that camels store water in their humps for long desert journeys. How would you modify this statement on the basis of information in this chapter?arrow_forwardREFLECT AND APPLY Why is the entropy of a system dependent on temperature?arrow_forward
- REFLECT AND APPLY You are studying with a friend who is describing the Bohr effect. She tells you that in the lungs, hemoglobin binds oxygen and releases hydrogen ion; as a result, the pH in- creases. She goes on to say that in actively metabolizing muscle tissue, hemoglobin releases oxygen and binds hydrogen ion and, as a result, the pH decreases. Do you agree with her reasoning? Why or why not?arrow_forwardREFLECT AND APPLY Consider the reaction Glucose-6-phosphate+H2OGlucose+PiKeq=[glucose][Pi][glucose-6-P] The Keq at pH 8.5 and 38C is 122. Can you determine the rate of the reaction from this information?arrow_forwardREFLECT AND APPLY Which would you think would be a stronger interaction and why: an interaction between a sodium ion and the partial negative charge on the oxygen in ethanol (CH3CH2OH), or the interaction between two ethanol molecules?arrow_forward
- REFLECT AND APPLY Why is water necessary for life?arrow_forwardREFLECT AND APPLY Give two reasons why enzyme catalysts are 103 to 105 more effective than reactions that are catalyzed by, for example, simple H+orOH.arrow_forwardREFLECT AND APPLY Why can we say that having a pure non- competitive inhibitor present is similar to just having less enzyme present?arrow_forward
- REFLECT AND APPLY Explain the difference between an SN1 reaction mechanism and an SN2 reaction mechanism.arrow_forwardREFLECT AND APPLLY Show that the reaction Glucose2Glycer-aldehyde-3- phosphate is slightly endergonic (G'52.2kJmol1=0.53kcalmol1); that is, it is not too far from equilibrium. Use the data in Table 17.1.arrow_forwardREFLECT AND APPLY Suggest a reason why the cell membranes of bacteria grown at 20C tend to have a higher proportion of unsaturated fatty acids than the membranes of bacteria of the same species grown at 37C. In other words, the bacteria grown at 37C have a higher proportion of saturated fatty acids in their cell membranes.arrow_forward
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