Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 6, Problem 11PDQ
Explain the observations that led Zinder and Lederberg to conclude that the prototrophs recovered in their transduction experiments were not the result of F+ mediated conjugation.
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A group of genetics students mix two auxotrophic strains of bacteria: one is leu+ trp+ his− met− and the other is leu− trp− his+ met+. After mixing the two strains, they plate the bacteria on minimal medium and observe a few prototrophic colonies (leu+ trp+ his+ met+). They assume that some gene transfer has taken place between the two strains. How can they determine whether the transfer of genes is due to conjugation, transduction, or transformation?
Chapter 6 Solutions
Concepts of Genetics (12th Edition)
Ch. 6 - When the interrupted mating technique was used...Ch. 6 - In a transformation experiment involving a...Ch. 6 - In complementation studies of the rII locus of...Ch. 6 - A 4-month-old infant had been running a moderate...Ch. 6 - Prob. 2CSCh. 6 - Prob. 3CSCh. 6 - Prob. 4CSCh. 6 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 6 - Review the Chapter Concepts list on p. 123. Many...Ch. 6 - With respect to F+ and F bacterial matings, answer...
Ch. 6 - List all major differences between (a) the F+ F...Ch. 6 - Describe the basis for chromosome mapping in the...Ch. 6 - In general, when recombination experiments are...Ch. 6 - Why are the recombinants produced from an Hfr F...Ch. 6 - Describe the origin of F bacteria and merozygotes.Ch. 6 - In a transformation experiment, donor DNA was...Ch. 6 - Describe the role of heteroduplex formation during...Ch. 6 - Explain the observations that led Zinder and...Ch. 6 - Prob. 12PDQCh. 6 - Two theoretical genetic strains of a virus (abc...Ch. 6 - The bacteriophage genome consists of many genes...Ch. 6 - If a single bacteriophage infects one E. coli cell...Ch. 6 - A phage-infected bacterial culture was subjected...Ch. 6 - In recombination studies of the rII locus in phage...Ch. 6 - In an analysis of rII mutants, complementation...Ch. 6 - If further testing of the mutations in Problem 18...Ch. 6 - Using mutants 2 and 3 from Problem 19, following...Ch. 6 - During the analysis of seven rII mutations in...Ch. 6 - In studies of recombination between mutants 1 and...Ch. 6 - Prob. 23ESPCh. 6 - An Hfr strain is used to map three genes in an...Ch. 6 - A plaque assay is performed beginning with 1 mL of...Ch. 6 - In a cotransformation experiment, using various...Ch. 6 - For the experiment in Problem 26, another gene, g,...Ch. 6 - Bacterial conjugation, mediated mainly by...Ch. 6 - A study was conducted in an attempt to determine...
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- Why would it have been possible to utilize N14 in the original bacteria and only allow growing bacteria to access N15 to build new (F1) bacteria and get the same results as the original Meselson experiment?arrow_forwardWhat is the toxin produced by E. coli O157:H7 that was encode by phage as a consequence of lysogenic conversion? Explain the medical importance.arrow_forwardSuppose that one species of bacteria obtained one gene encoding a restriction endonuclease by horizontal gene transfer. Would you expect this acquisition to be beneficial?arrow_forward
- Why is Agrobacterium mediated genetic transformation described as Natural Genetic engineering in plants?arrow_forwardIn a rapidly changing environment, which bacterial population would likely be more successful: one that has individuals capable of conjugation or one that does not? Explain.arrow_forwarda bacteriophage has been isolated from the growth chambers that has evolved the capability to the methylate its own DNA when it is replicated inside the host bacterium. why is this a problem?arrow_forward
- Austin Taylor and Edward Adelberg isolated some new strains of Hfr cells that they then used to map several genes in Escherichia coli by using interrupted conjugation. In one experiment, the researchers mixed cells of Hfr strain AB‑312, which were xyl+ mtl+ mal+ met+ and sensitive to phage T6, with F− strain AB‑531, which was xyl− mtl− mal− met− and resistant to phage T6. The cells were allowed to undergo conjugation. At regular intervals, the researchers removed a sample of cells and interrupted conjugation by killing the Hfr cells with phage T6. The F− cells, which were resistant to phage T6, survived and were then tested for the presence of genes transferred from the Hfr strain. The results of this experiment are shown in the graph. On the basis of these data, give the order of the xyl, mtl, mal, and met genes on the bacterial chromosome and the minimum distances between them in minutes. The origin of transfer is represented by the red triangle. The distances between genes are not…arrow_forwardAustin Taylor and Edward Adelberg isolated some new strains of Hfr cells that they then used to map several genes in E. coli by using interrupted conjugation . In one experiment, they mixed cells of Hfr strain AB-312, which were xyl+ mtl+ mal+ met+ and sensitive to phage T6, with F− strain AB-531, which was xyl− mtl− mal− met− and resistant to phage T6. The cells were allowed to undergo conjugation. At regular intervals, the researchers removed a sample of cells and interrupted conjugation by killing the Hfr cells with phage T6. The F− cells, which were resistant to phage T6, survivedand were then tested for the presence of genes transferred from the Hfr strain. The results of this experiment are shown in the accompanying graph. On the basis of these data, give the order of the xyl, mtl, mal, and met genes on the bacterial chromosome and indicate the minimum distances between them.arrow_forwardYou mix two auxotrophic strains of bacteria; one is leu+ trp+ his– met– and the other is leu– trp– his+ met+. After mixing the two strains, you plate the bacteria on minimal medium and observe a few prototrophic colonies (leu+ trp+ his+ met+). To determine whether gene transfer has occurred through conjugation, transformation, or transduction, you design an experiment in which you place filters between the two strains to see if gene transfer occurs. You set up three treatments:-Treatment A No filter, allowing contact between the bacterial strains-Treatment B Filter that blocks cells, but allows viruses and DNA through the filter-Treatment C Filter that blocks cells and viruses, but allows DNA through the filter When you check the results of your experiment, you find prototrophic colonies have occurred following treatments A and B, but not following treatment C. You conclude that gene transfer occurred through... Answer Choices Available: A) Conjugation B) Transformation C)…arrow_forward
- When would it be beneficial to use a eukaryotic host formolecular cloning?arrow_forwardExplain why recipient cells do not successfully takeup plasmids during natural transformation.arrow_forwardWhat is the role of the origin of transfer during F+- and Hfr-mediated conjugation? What is the significance of the directionof transfer in Hfr-mediated conjugation?arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license