Concepts of Genetics (12th Edition)
12th Edition
ISBN: 9780134604718
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino, Darrell Killian
Publisher: PEARSON
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Chapter 6, Problem 24ESP
An Hfr strain is used to map three genes in an interrupted mating experiment. The cross is Hfr/a+b+c+rif × F−>a−b−c−rifr. (No map order is implied in the listing of the alleles; rifr is resistance to the antibiotic rifampicin.) The a+ gene is required for the biosynthesis of nutrient A, the b+ gene for nutrient B, and c+ for nutrient C. The minus alleles are auxotrophs for these nutrients. The cross is initiated at time = 0, and at various times, the mating mixture is plated on three types of medium. Each plate contains minimal medium (MM) plus rifampicin plus specific supplements that are indicated in the following table. (The results for each time interval are shown as the number of colonies growing on each plate.)
- (a) What is the purpose of rifampicin in the experiment?
- (b) Based on these data, determine the approximate location on the chromosome of the a, b, and c genes relative to one another and to the F factor.
- (c) Can the location of the rif gene be determined in this experiment? If not, design an experiment to determine the location of rif relative to the F factor and to gene b.
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An Hfr strain is used to map three genes in an interrupted mating experiment. The cross is Hfr/a+b+c+ rif x F-a-b-c- rifr (note that rifr is resistance to the antibiotic rifampicin). The a+ gene is required for the biosynthesis of nutrient A, the b+ gene for nutrient B, and the c+ gene for nutrient C. The cross is initiated at time=0. At various times, the mating mixture is plated out on three different types of medium. Each plate contains minimal medium (MM) plus rifampicin, plus specific supplements that are listed in the table below. The results for each time interval are shown as the number of colonies growing on each plate.An Hfr strain is used to map three genes in an interrupted mating experiment. The cross is Hfr/a+b+c+ rif x F-a-b-c- rifr (note that rifr is resistance to the antibiotic rifampicin). The a+ gene is required for the biosynthesis of nutrient A, the b+ gene for nutrient B, and the c+ gene for nutrient C. The cross is initiated at time=0. At various times, the…
Four Hfr strains are derived from an F+ strain of E. Coli to serve as donors for an interrupted-mating experiment. Use the time-table and partial map of the F+ strain (shown below) to determine the genes’ respective positions. Keep in mind that the map distances are NOT proportional, only the FIRST 5 markers are indicated per strain, and the entry times, recorded in minutes, are in parentheses. Transferred genes represent wild-type alleles. Based on the data, which gene can be located at position 5 on the map?
In five Hfr strains, each of which was used to build a time-of-transfer map, the genes entered the recipient cells as follows:
Strain 1: S L A C T F
Strain 2: N P F T C A
Strain 3: T F P N U Y
Strain 4: S H Y U N P
Strain 5: U N P F T C
Which of the following represents a correct gene map of these results?
N P F T S L A C H U Y
S L A C T F P N H Y U
C T F P N U Y H S L A
T C A L S P N U Y H F
U N P C A L S F T H Y
Chapter 6 Solutions
Concepts of Genetics (12th Edition)
Ch. 6 - When the interrupted mating technique was used...Ch. 6 - In a transformation experiment involving a...Ch. 6 - In complementation studies of the rII locus of...Ch. 6 - A 4-month-old infant had been running a moderate...Ch. 6 - Prob. 2CSCh. 6 - Prob. 3CSCh. 6 - Prob. 4CSCh. 6 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 6 - Review the Chapter Concepts list on p. 123. Many...Ch. 6 - With respect to F+ and F bacterial matings, answer...
Ch. 6 - List all major differences between (a) the F+ F...Ch. 6 - Describe the basis for chromosome mapping in the...Ch. 6 - In general, when recombination experiments are...Ch. 6 - Why are the recombinants produced from an Hfr F...Ch. 6 - Describe the origin of F bacteria and merozygotes.Ch. 6 - In a transformation experiment, donor DNA was...Ch. 6 - Describe the role of heteroduplex formation during...Ch. 6 - Explain the observations that led Zinder and...Ch. 6 - Prob. 12PDQCh. 6 - Two theoretical genetic strains of a virus (abc...Ch. 6 - The bacteriophage genome consists of many genes...Ch. 6 - If a single bacteriophage infects one E. coli cell...Ch. 6 - A phage-infected bacterial culture was subjected...Ch. 6 - In recombination studies of the rII locus in phage...Ch. 6 - In an analysis of rII mutants, complementation...Ch. 6 - If further testing of the mutations in Problem 18...Ch. 6 - Using mutants 2 and 3 from Problem 19, following...Ch. 6 - During the analysis of seven rII mutations in...Ch. 6 - In studies of recombination between mutants 1 and...Ch. 6 - Prob. 23ESPCh. 6 - An Hfr strain is used to map three genes in an...Ch. 6 - A plaque assay is performed beginning with 1 mL of...Ch. 6 - In a cotransformation experiment, using various...Ch. 6 - For the experiment in Problem 26, another gene, g,...Ch. 6 - Bacterial conjugation, mediated mainly by...Ch. 6 - A study was conducted in an attempt to determine...
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