Biology
Biology
5th Edition
ISBN: 9781260487947
Author: BROOKER
Publisher: MCG
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Chapter 6, Problem 4TY

Researchers analyzed a cell extract—a mixture of molecules isolated from a certain type of cell—and studied a chemical reaction in which a carbohydrate was broken down into smaller molecules.

When they added a protease to the cell extract, they discovered that the protease greatly inhibited the rate of the reaction. Based on this observation, you could conclude that the reaction is

  1. a. exergonic.
  2. b. endergonic.
  3. c. catalyzed by an enzyme.
  4. d. catalyzed by a ribozyme.
  5. e. Both b and c are true of this reaction.
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What is the reason why the transition state of a catalyzed reaction is lower has lower energy compared to an uncatalyzed reaction? A. because enzymes only work at low temperatures B. because of favorable interactions with the substrate C. because of enthalpic interactions between the enzyme and the transition state D. A and B
The probabiliy of a particular reaction occurring in a cell can be increased by A) having reactants contained within an organelle B) having reactants contained within an organelle with the necessary enzymes C) having low concentrations of the reactants D) having reactants with enthalphy with high enthalpy product(s)
Refer to the figure below and answer the following questions: Legend: Blue – wild-type β-galactosidase; Red – mutant β-galactosidase   _________ a. What is the optimum pH of wild type β-galactosidase?         _________ b. What is the optimum temperature of mutant β-galactosidase?         _________ c. Which enzyme has the greater activity at pH 7.2? _________ d. Which enzyme has the greater activity at a temperature of 42.5oC? _________ e. Which enzyme has greater activity if pH decreases from 7.5 to 6.4?            _________ f. Which enzyme has greater activity if temperature increases from 40oC to 41 oC?
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