(a)
Interpretation: The component of the sample that elutes faster needs to be determined. The reported boiling points of these substances need to be determined.
Concept Introduction:
Gas chromatography is used to separate the components of a sample mixture. There are two phases that is stationary and mobile. The mobile phase flows with the stationary phase which is the phase on which compounds of the mixture that needs to be separated are selectively absorbed. The mobile phase carries these compounds.
(b)
Interpretation: The GLC experiment that should be performed to identified the peaks at 9.56 and 16.23 minutes needs to be determined.
Concept Introduction:
Gas chromatography is used to separate the components of a sample mixture. There are two phases that is stationary and mobile. The mobile phase flows with the stationary phase which is the phase on which compounds of the mixture that needs to be separated are selectively absorbed. The mobile phase carries these compounds.
(c)
Interpretation: The organic compound in bear responsible for the peak needs to be determined and explained.
Concept Introduction:
Gas chromatography is used to separate the components of a sample mixture. There are two phases that is stationary and mobile. The mobile phase flows with the stationary phase which is the phase on which compounds of the mixture that needs to be separated are selectively absorbed. The mobile phase carries these compounds.
(d)
Interpretation: The two ways that should be suggested to reduce the retention time for all the components of the sample needs to be explained.
Concept Introduction:
Gas chromatography is used to separate the components of a sample mixture. There are two phases that is stationary and mobile. The mobile phase flows with the stationary phase which is the phase on which compounds of the mixture that needs to be separated are selectively absorbed. The mobile phase carries these compounds.
Want to see the full answer?
Check out a sample textbook solutionChapter 6 Solutions
EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
- Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spotswere detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. If the TLC chromatogram in the picture was duplicated but using a column, draw the set-up and label approximate spots.arrow_forwardA thin-layer chromatography (TLC) experiement was run using three compunds: benzophenone, benzhydrol, and biphenyl. The three solvents that were used with the three compounds were hexane, ethyl acetate, and dichloromethane. Which solvent would be the best for separating these compounds and why?arrow_forwardDoes the presence of a benzene ring or carboxylic acid functional group in a substance affect the retention factor (Rf) when doing TLC on silica gel ?arrow_forward
- In an experiment of Reverse Phase chromatography using C-18 stationary phase and a mixture of methanol/ethyl acetate/acetic acid, a researcher tried to separate the 3 compounds shown below. Which compound would have the lowest Rf and which the highest Rf in this experiment? Explain your answer and show any relevant structures.arrow_forwardAmino acids and fatty acids do not readily elute from gas chromatography columns even at temperatures above 200 degrees Celsius. What can be done to these biomolecules to allow gas chromatographic analysis?arrow_forwardWhen separating benzil and benzoin in column chromatography, why do you start with 5% ethyl acetate in hexane and then switch to 30% ethyl acetate in hexane.arrow_forward
- You spotted a mixture of three compounds, labeled A-C, on a silica plate. The resulting thin layer chromatography (TLC) plate with a mobile solvent mixture of 95 % hexanes and 5 % ethyl acetate is shown below. Which of the spots traveled the furthest up the TLC plate corresponding to spot 3?arrow_forwardA student used thin layer chromatography to confirm whether he could successfully separate two compounds. Here is the TLC plate he obtained after 30 min. Stationary phase was silica gel and mobile phase was 95 % ethyl acetate and 5 % Hexane mixture.arrow_forwardA student places an unknown sample on a thin-layer chromatography plate and develops it in dichloromethane as a solvent. Only one spot whose Rf is 0.98 is observed. Does this result indicate that the unknown material is a pure compound? If not a pure compound, suggest an alternate procedure to verify the purity of the sample.arrow_forward
- EBK A SMALL SCALE APPROACH TO ORGANIC LChemistryISBN:9781305446021Author:LampmanPublisher:CENGAGE LEARNING - CONSIGNMENTPrinciples of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning