(a)
Interpretation:
Most polar phase should be identified from mobile phase and stationary phase in reverse phase column chromatography.
Concept Introduction :
Column chromatography is a large scale solid-liquid adsorption chromatographic technique that can separate multi-gram amounts of mixtures. This is a preparative version of thin layer chromatography that permits separation and isolation of products by collection of eluted fractions.
There are two types of column chromatography named as normal phase-column chromatography and reverse − phase column chromatography.
(b)
Interpretation:
Reverse-phase chromatography is more appropriate in separating peptides than normal-phase chromatography should be explained.
Concept Introduction :
Column chromatography is a large scale solid-liquid adsorption chromatographic technique that can separate multi-gram amounts of mixtures. This is a preparative version of thin layer chromatography that permits separation and isolation of products by collection of eluted fractions.
There are two types of column chromatography named as normal phase-column chromatography and reverse − phase column chromatography.
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Chapter 6 Solutions
EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
- a) What would you see if you ran a Thin Layer Chromatography (TLC) on a mixture of the three solids shown below using silica gel as the stationary phase and ethyl acetate/hexanes as the mobile phase? Whichsolid would have the highest Rf and which would have the lowest Rf? Explain b) Would changing the mobile phase (from part a) and increasing thepolarity by introducing small amount of methanol to the mobile phase change the order ofRf of the above 3 compounds? Explain c) Another type of chromatography is Reverse Phase Chromatography. In this type ofchromatography, the stationary phase is non-polar and the mobile phase is polar. If astudent performed Reverse Phase TLC on the mixture of 3 solids (from part a),what would be the order of Rf’s of the compounds? Which would have the lowest Rf andwhich would have the highest Rf?arrow_forwardIndicate whether TLC (thin-layer chromatography) or column chromatography is more appropriate for the following:a. This is an analytical method for separating compounds of amixture.b. The solvent front moves downward.c. This is a preparative method for separating a mixture ofcompounds.arrow_forwardWhat is the structure and IUPAC name of atrazine? The herbicide simazine is more polar than atrazine. In reverse phase chromatography of a solution containing a mixture of the two substances, which would elute first? Explain why this is in terms of partition. The instrument used in this practical exercise has a UV spectrophotometer as a detector. Give two other types of chromatographic detectors that might be used.arrow_forward
- 28. In addition to what is not a characteristic of gas chromatography as listed in the probelm can you give the general characteristics of Gas Chromatography?arrow_forwardIn paper chromatography, a rectangular piece of filter paper serves as the mobile phase stationary phase eluent solute sample spotsarrow_forwardConsider stereoisomers. What type of isomers would you expect would and what types could not be separted by chromatography techniques. also include specifically thin layer chromotography and why, explainarrow_forward
- In kool aid chromatography, why does reducing the dilution of 2-propanol result in its decrease in polarity?arrow_forwardDifferentiate normal phase chromatography from reverse phase chromatography. From which of the two in TLC and paper chromatography is normal phase chromatography and reverse phase chromatography? How do you say so?arrow_forward
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