(a)
Interpretation:
The Rf values of acetaminophen and caffeine should be determined.
Concept Introduction :
In chromatography, Rf value is the ratio between distance travelled by the component and distance travelled by the solvent front.
(b)
Interpretation:
The ingredients in a tablet of Midol should be determined based on the TLC analysis.
Concept Introduction :
Thin layer chromatography is a separation technique used for non-volatile substances. Depending on the affinity of the component of the mixture to the stationary phase and mobile phase, distance travelled by each component on the TLC plate differ.
(c)
Interpretation:
The mobile phase and stationary phase in the TLC experiment should be determined.
Concept Introduction :
All chromatography techniques have a stationary phase and a mobile phase. Stationary phase is a solid, or a liquid supported on a solid. Stationary phase is not migrating. The mobile phase flows through the stationary phase and carries components along with it.
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EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
- Why are peak areas used instead of peak height in Reverse Phase HPLC of Caffeine and Benzoic Acid?arrow_forwardOf Aspirin, ethanol, acetaminophen, ethyl acetate, hexane, and water which could go undetected during HPLC analysis, but could result in a lower than expected melting point, why are they not detected by HPLC analysis?arrow_forwarda) What would you see if you ran a Thin Layer Chromatography (TLC) on a mixture of the three solids shown below using silica gel as the stationary phase and ethyl acetate/hexanes as the mobile phase? Whichsolid would have the highest Rf and which would have the lowest Rf? Explain b) Would changing the mobile phase (from part a) and increasing thepolarity by introducing small amount of methanol to the mobile phase change the order ofRf of the above 3 compounds? Explain c) Another type of chromatography is Reverse Phase Chromatography. In this type ofchromatography, the stationary phase is non-polar and the mobile phase is polar. If astudent performed Reverse Phase TLC on the mixture of 3 solids (from part a),what would be the order of Rf’s of the compounds? Which would have the lowest Rf andwhich would have the highest Rf?arrow_forward
- 1. What factors can affect a solute's Rf value in thin-layer chromatography?2. Why is it necessary to follow these steps when conducting the experiment? -No contact with the TLC plate should be made with bare hands.-Spots should have a diameter of no more than 1 mm.-Allow sufficient solvent to saturate the chromatography chamber.arrow_forward1. What is paper chromatograpy?2. What are the stationary phase and mobile phase in paper chromatograpy?3. What is the principle underlying the paper chromatograpy?4. What is Rf value of a dye or pigment?5. Would you expect that changing the solvent will change the Rf value of the specific solute? Why?6. Suggest simple reasons why the Ni2+ and Fe3+ ions have different Rf values?7. What forces causes the eluting solution to move the chromatographic support material?arrow_forwardIn TLC, why does a 3:1:1 ratio of the developing solvent of n-butanol/acetic acid/water move up the TLC plate so slowly?arrow_forward
- The three TLC plates below show the analysis of the same mixture of two compounds. One plate was developed in 100% DCM, one in 90:10 DCM/MeOH, and one in 80:20 DCM/MeOH. Determine which plate was developed in which solvent.arrow_forwardA student places an unknown sample on a thin-layer chromatography plate and develops it in dichloromethane as a solvent. Only one spot whose Rf is 0.98 is observed. Does this result indicate that the unknown material is a pure compound? If not a pure compound, suggest an alternate procedure to verify the purity of the sample.arrow_forwardThin Layer Chromatography why the non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value)? Please help me explain this question! Thank you so much!arrow_forward
- 1. In thin layer chromatography (TLC), why do you need to use a pencil in marking the spots, origin, and solvent front and not a marker? 2. Why is it necessary to mark the solvent front immediately? 3. Why do you need to ensure that the loading spot is above the solvent level in paper chromatography? 4. Why do different compounds travel different distances on the piece of paper?arrow_forward1- What are the main two types of columns in the HPLC? 2-What are the most disadvantages of mass-spectrmeter as a detector for HPLC? 3-What Are The Commonly Used Carrier Gases In GC Analysis ? 4-Name four of the mechanisms of the separation in chromatography? 5-Which Is The Most Commonly Used Detector In High Performance Liquid Chromatography And Why?arrow_forwardHow does increasing the polarity of the developing solvent affect the Rf of a compound on a silica gel TLC plate?arrow_forward
- EBK A SMALL SCALE APPROACH TO ORGANIC LChemistryISBN:9781305446021Author:LampmanPublisher:CENGAGE LEARNING - CONSIGNMENT