Concept explainers
(a)
Interpretation:
The graph of the given data to form an oxygen-binding curve is to be plotted. The oxygen partial pressure at which the given hemoglobin is half-saturated is to be stated. Whether oxygen binding seems to be cooperative or not on the basis of the curve is to be stated.
Concept introduction:
Proteins are the
Answer to Problem 16P
The plot of the given data to form an oxygen-binding curve is shown below.
The oxygen partial pressure at which the given hemoglobin is half-saturated is
Explanation of Solution
On the basis of the given data for oxygen-binding for lamprey hemoglobin, the graph of fractional saturation versus partial pressure of oxygen is plotted as follows:
At the oxygen partial pressure,
Thus, this plot between partial pressure of oxygen
(b)
Interpretation:
A hill plot by using the given data is to be stated. Whether the hill plot shows any evidence for cooperativity or not is to be stated. The information about the hill coefficient is to be explained.
Concept introduction:
Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.
The hill equation indicates the two close equations which show the binding of a ligand with the macromolecule.
Answer to Problem 16P
A hill plot by using the given data is shown as,
This plot show little bit cooperativity in the center of the curve and its hill coefficient is
Explanation of Solution
The equation that is used to show the hill equation is given as,
Here,
The values of
Similarly, for
The value of
Similarly, for
With the help of the given data for oxygen-binding for lamprey hemoglobin, the plot between
The value of slope
The hill coefficient for the graph is
(c)
Interpretation:
A model that is used to explain the observed cooperativity in oxygen binding by lamprey hemoglobin is to be stated.
Concept introduction:
Proteins are the biomolecules which are composed of the long chain of amino acid residues. The protein which contains oxygen and is present in the red blood cells in the body is known as hemoglobin. It contains iron as well.
Answer to Problem 16P
A model that is used to explain the observed cooperativity in oxygen binding by lamprey hemoglobin suggests that binding of oxygen with any monomer is easier as compared to binding of first oxygen with deoxygenated dimer.
Explanation of Solution
It is given that lamprey hemoglobin produces oligomers in the deoxygenated state. These oligomers were primarily dimmers in the deoxygenated state. These dimers are having low affinity for oxygen as compared to monomers. The cooperativity in oxygen binding is shown only if the dissociation of dimmers into two monomers occurs after the binding of first oxygen atom with a dimer. In this model, the binding of oxygen with every monomer is very easy as compared to the binding between first oxygen and the deoxy dimer.
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Chapter 7 Solutions
BIOCHEMISTRY 2 TERM ACCESS
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- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning