EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
expand_more
expand_more
format_list_bulleted
Concept explainers
Textbook Question
Chapter 7, Problem 17CT
Suppose you want to insert into your dog a gene that encodes a protein that protects dogs from heartworms. A dog’s cells are not competent, so they cannot take up the gene from the environment; but you have a plasmid, a competent bacterium, and a related (though incompetent) F+ bacterium that lives as an intracellular
Expert Solution & Answer
![Check Mark](/static/check-mark.png)
Trending nowThis is a popular solution!
![Blurred answer](/static/blurred-answer.jpg)
Students have asked these similar questions
The genes for both the α- and βglobin chains of hemoglobin contain introns (i.e., they are split genes). How would this fact affect your plans if you wanted to introduce the gene for α-globin into a bacterial plasmid and have the bacteria produce α-globin?
Imagine that there is an E. coli outbreak in your area, and you would like to test the kangkong from your local grocery store. How could you modify this protocol to extract DNA from the kangkong (to identify the species) and check for presence or absence of E. coli.? Keep in mind that (i) E. coli is free-living and not an endosymbiont, and (ii) plant cells are encased in both a cell membrane and cell wall.
Materials
In order to determine the genetic material of a T2 phage, Alfred Hershey and
Martha Chase conducted experiments using T2 phages that infected bacteria. In
one treatment, they grew phages with radioactive sulfur. In another treatment,
they grew phages with radioactive phosphorous. They allowed both types of
phages to infect bacterial cells. After infection, they found that only bacteria
infected with phages grown with radioactive phosphorous showed any
radioactivity. Why did they use radioactive sulfur and phosphorous for this
Updates
Grades
Members
O Conferences
DBQ Online
experiment? *
Newsela
ormation
O Sulfur is part of the DNA molecule but not part of a protein molecule.
Biology Periods 1 and 2
Sulfur and phosphorous are some of the most reactive molecules and are easily
ding periods
school MP1, Highschool
Highschool MP3,
school MP4
traced.
Sulfur and phosphorous are able to survive the centrifuge, a crucial component of the
experiment.
ion
Phosphorous is part of the DNA…
Chapter 7 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 7 - DNA replication requires a large amount of energy,...Ch. 7 - Vibrio vulnificus Infection Greg enjoyed Floridas...Ch. 7 - Prob. 2TMWCh. 7 - Prob. 3TMWCh. 7 - Why is the genetic ancestry of microbes much more...Ch. 7 - Prob. 1CCSCh. 7 - Which of the following is most likely the number...Ch. 7 - Which of the following is a true statement...Ch. 7 - A plasmid is ___________. a. a molecule of RNA...Ch. 7 - Prob. 4MC
Ch. 7 - Prob. 5MCCh. 7 - Which of the following molecules functions as a...Ch. 7 - Prob. 7MCCh. 7 - Prob. 8MCCh. 7 - The Ames test ___________. a. uses auxotrophs and...Ch. 7 - Which of the following methods of DNA repair...Ch. 7 - Prob. 11MCCh. 7 - Prob. 12MCCh. 7 - Which of the following statements is true? a....Ch. 7 - Prob. 14MCCh. 7 - Although two cells are totally unrelated, one cell...Ch. 7 - Prob. 16MCCh. 7 - Prob. 17MCCh. 7 - Prob. 18MCCh. 7 - Prob. 19MCCh. 7 - Prob. 20MCCh. 7 - Prob. 21MCCh. 7 - Prob. 22MCCh. 7 - Prob. 23MCCh. 7 - Before mutations can affect a population...Ch. 7 - Prob. 25MCCh. 7 - Fill in the Blanks 1. The three steps in RNA...Ch. 7 - Fill in the Blanks 2. A triplet of mRNA...Ch. 7 - Fill in the Blanks 3. Three effects of point...Ch. 7 - Fill in the Blanks 4. Insertions and deletions in...Ch. 7 - Fill in the Blanks 5. An operon consists of...Ch. 7 - Prob. 6FIBCh. 7 - Prob. 7FIBCh. 7 - Fill in the Blanks 8. A gene for antibiotic...Ch. 7 - Fill in the Blanks 9. ______ are nucleotide...Ch. 7 - Fill in the Blanks 10. ____________ is a...Ch. 7 - Fill in the Blanks 11.________ RNA carries amino...Ch. 7 - Fill in the Blanks 12. ______ RNA and ______ RNA...Ch. 7 - How does the genotype of a bacterium determine its...Ch. 7 - List several ways in which eukaryotic messenger...Ch. 7 - Compare and contrast intrans and exons.Ch. 7 - Polypeptide synthesis requires large amounts of...Ch. 7 - Describe the operon model of gene regulation.Ch. 7 - Prob. 6SACh. 7 - Prob. 7SACh. 7 - Describe the formation and function of mRNA, rRNA,...Ch. 7 - Prob. 9SACh. 7 - Explain the central dogma of genetics.Ch. 7 - Compare and contrast the processes of...Ch. 7 - Fill in the following table:Ch. 7 - On the figure below, label DNA polymerase I, DNA...Ch. 7 - Prob. 2VICh. 7 - The drugs ddC and AZT are used to treat AIDS....Ch. 7 - If molecules of mRNA have the following nucleotide...Ch. 7 - A scientist uses a molecule of DNA composed of...Ch. 7 - Explain why an insertion of three nucleotides is...Ch. 7 - How could scientists use siRNA to turn off a...Ch. 7 - Prob. 5CTCh. 7 - Prob. 6CTCh. 7 - Prob. 7CTCh. 7 - Prob. 8CTCh. 7 - Corynebacterium diphtheriae, the causative agent...Ch. 7 - Prob. 10CTCh. 7 - The endosymbiotic theory states that mitochondria...Ch. 7 - Hydrogen bonds between complementary nucleotides...Ch. 7 - On average, RNA polymerase makes one error for...Ch. 7 - We have seen that wobble makes the genetic code...Ch. 7 - If a scientist synthesizes a DNA molecule with the...Ch. 7 - What DNA nucleotide triplet codes for codon UGU?...Ch. 7 - Suppose you want to insert into your dog a gene...Ch. 7 - Using the following terms, fill in the following...
Knowledge Booster
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Researchers are designing several experiments to test the ability of Salmonella bacteria to develop antibiotic resistance. A culture of Salmonella bacteria is exposed to the same concentrations (200 mg/L) of an antibiotic for four days. The table shows the number of isolated resistant bacteria over a four-day period. Which of the following statements best explains these results? A - The bacteria were not affected by the antibiotic. B - After being exposed to the antibiotic, the bacteria altered their DNA. C - A new species of bacteria emerged after the antibiotics were introduced. D - Random mutations led some bacteria to be resistant and, over time, they increased in the population.arrow_forwardBacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: The cas genes on the bacterial genome contains a frameshift deletion mutation that alters the function of the protein The bacteria will be unable to elicit an immune response and will succumb to the phase infectionarrow_forwardWe transformed E coli cells with a plasmid modified to contain a ‘virulence factor’ which would allow growth on media containing the antibiotic kanamycin (Kan). The plasmid confers constitutive resistance to ampicillin (Amp). The bacterial experiment is about understanding whether such a ‘virulence factor’ confers physiological adaptation to Kan or whether the development of resistance can be explained by random mutations. For each independent transformation we re-suspended the cells from three colonies in Luria broth. For each suspension of cells we plated 100 microliters on a Kan plate. To estimate the number of cells seeded on each Kan plate we made four serial dilutions that were plated on Amp plates (1 – 4) and we counted the number of cells growing on them. From this we extrapolated how many cells had been seeded on the Kan plate. Then we normalised the Kan results for all the plates, assuming that every plate had been seeded with 10[5] cells. Consider two Kan plates, each with…arrow_forward
- You have found a gene of interest in an animal in the rain forest which produces a unique protein that will blocks reproduction of the pathogen that now plagues the world. You wish to mass produce this protein using bacteria. Answer the following questions regarding the scenario above. Describe how you would remove the gene of interest out of the isolated animal cell. What type of vector, (delivery system), would be best used to get the gene into the bacteria? How would you insert the gene of interest into the vector? How would get the vector into the bacteria? Why would the bacteria express this gene even if it normally does not have use for this protein?arrow_forwardA hypothetical gene for cephalosporin resistance is found to be carried by a transposon. Explain what a transposon is. Then explain how the cephalosporin resistance could be horizontally transferred between organisms by transformation, conjugation, and transduction. What steps/events would have to occur to allow the transposon to be transferred by each method. Also, explain how it could be transferred vertically between organisms.arrow_forwardBacterial conjugation has medical consequences. For example, certain plasmids contain genes that code for toxins. The causative agent of a deadly form of food poisoning, E. coli 0157, synthesizes a toxin that causes massive bloody diarrhea and kidney failure. This toxin is now believed to have originated in Shigella, another bacterium that causes dysentery. Similarly, the growing problem of antibiotic resistance is partly attributable to the spread of antibioticresistant genes among bacterial populations. Antibiotic resistance develops because antibiotics are overused in medical practice and in livestock feeds. Suggest a mechanism by which this extensive use promotes antibiotic resistance.arrow_forward
- Considering the proclivity of bacteria to share genetic information, discuss the concerns behind phenomena such as antibiotic resistance. In other words, why might it be a bad idea to not finish your antibiotic therapy as prescribed by your physician? Some people think that "saving some for later" is a good idea. Why might that not be the case?arrow_forwardBacteria can be used to produce human growth hormone (HGH - a peptide/protein) through genetic engineering. The human gene for HGH is inserted into a plasmid, which is then taken up by a bacterial cell, which divides and multiplies into a clone of cells, all of which contain the plasmid with the HGH gene. The bacteria express the HGH gene, producing HGH which can be harvested and used for treatment of humans. (See figure below) Which of the following statements is NOT true about this process? bacterium Vector, such as a DNA containing the gene of plasmid, isolated it from a different species is Gene encoding protein for pest resistance is inserted into plant cells ©2019 Pearson Education, Inc chromosome recombinant DNA (plasmid) transformed bacterium Create and harvest copies of a gene with either of two goals in mind. Gene encoding degradative enzyme to clean up toxdo waste is inserted into bacterial cells ved by an enzyme into gene of interest The desired gene is selected and…arrow_forwardIn a hypothetical scenario, you wake up one morning to your roommate exclaiming about her sudden hair growth. She has been supplementing her diet with a strange new fungus purchased at the local farmer’s market. You take samples of the fungus to your lab and you find that this fungus does indeed make a protein (the harE protein) that stimulates hair growth. You construct a fungal genomic DNA library in E. Coli with the hope of cloning the harE gene. If you succeed you will be a billionaire! You obtain DNA from the fungus, digest it with a restriction enzyme, and clone it into a vector. b) You clone your digested genomic DNA into this vector. The E. coli (bacteria) cells that you will transform to create your library will have what phenotype prior to transformation?c) How do you distinguish bacterial cells that carry a vector from those that do not?d) How do you distinguish bacterial cells that carry a recombinant vector from those that carry the original cloning vector?arrow_forward
- A strain of Neisseria gonorrheae has recently acquired the ability to be resistant to the antibiotic penicillin. Evidence indicates that this is not a new mutation but rather a gene received from another bacterium. After the new genetic information recombines with the genome, how would gonorrheae use the DNA to resist the action of penicillin? The gene would be transcribed into RNA and then translated into protein. The gene would translated into protein and then transcribed into RNA. The gene would be recognized as a mutation and repaired. The gene would be released from the N. gonorrheae as naked DNA.arrow_forwardWhy are bacterial DNA gyrase and topoisomerase good targets for antimicrobial drugs? These enzymes are not necessary for DNA replication. These enzymes are involved in energy production, so targeting them would prevent the bacteria from obtaining necessary energy. These enzymes are different from their eukaryotic counterparts, so the drugs targeting them should not affect the host. These enzymes are responsible for host cell recognition, so targeting them would prevent the host cell from being infected.Flag this QuestionQuestion 10arrow_forwardSuppose that you used reverse transcriptase to copy the ovalbumin mRNA and cloned this complementary DNA (CDNA) into a plasmid vector. Would you expect this CDNA to reanneal more slowly, more rapidly, or at the same rate as genomic DNA? Briefly explain your answer. Match the words in the left column to the appropriate blanks in the sentences on the right. Reset Help less The genomic DNA would renature CDNA because it has at the same rate as complexity among the 300-bp fragments used for the analysis. greater more slowly than more rapidly than the samearrow_forward
arrow_back_ios
SEE MORE QUESTIONS
arrow_forward_ios
Recommended textbooks for you
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education
![Text book image](https://www.bartleby.com/isbn_cover_images/9780134580999/9780134580999_smallCoverImage.gif)
Human Anatomy & Physiology (11th Edition)
Biology
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:PEARSON
![Text book image](https://www.bartleby.com/isbn_cover_images/9781947172517/9781947172517_coverImage_Textbooks.gif)
Biology 2e
Biology
ISBN:9781947172517
Author:Matthew Douglas, Jung Choi, Mary Ann Clark
Publisher:OpenStax
![Text book image](https://www.bartleby.com/isbn_cover_images/9781259398629/9781259398629_smallCoverImage.gif)
Anatomy & Physiology
Biology
ISBN:9781259398629
Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa Stouter
Publisher:Mcgraw Hill Education,
![Text book image](https://www.bartleby.com/isbn_cover_images/9780815344322/9780815344322_smallCoverImage.gif)
Molecular Biology of the Cell (Sixth Edition)
Biology
ISBN:9780815344322
Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter Walter
Publisher:W. W. Norton & Company
![Text book image](https://www.bartleby.com/isbn_cover_images/9781260159363/9781260159363_smallCoverImage.gif)
Laboratory Manual For Human Anatomy & Physiology
Biology
ISBN:9781260159363
Author:Martin, Terry R., Prentice-craver, Cynthia
Publisher:McGraw-Hill Publishing Co.
![Text book image](https://www.bartleby.com/isbn_cover_images/9781260231700/9781260231700_smallCoverImage.gif)
Inquiry Into Life (16th Edition)
Biology
ISBN:9781260231700
Author:Sylvia S. Mader, Michael Windelspecht
Publisher:McGraw Hill Education
genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license