Genetic Analysis: An Integrated Approach (2nd Edition)
2nd Edition
ISBN: 9780321948908
Author: Mark F. Sanders, John L. Bowman
Publisher: PEARSON
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Chapter 7, Problem 28P
In a dideoxy DNA sequencing experiment, four separate reactions are carried out to provide the replicated material for DNA sequencing gels. Reaction products are usually run in gel lanes labeled A,T,C, and G.
Identify the
a. How does PCR play a role in dideoxy DNA sequencing?
b. Why is incorporation of a dideoxynucleotide during DNA sequencing identified as a “replication-terminating” event?
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Students have asked these similar questions
You are trying to clone a gene, You have successfully isolated it from the genomic DNA of an organism using the Hindill restriction enzyme. You then take a
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a. Does the cloning reaction succeed as described? If so, what is the product obtained?
b. Explain your answer above,
What is expected theoretical number of copies of DNA molecules after 28 cycles in a PCR experiment? What is the percent efficiency of the PCR experiment if the actual number of copies was 500,000,000? Show your calculations
When performing Sanger sequencing a scientist accidentally forgot to include ddGTP in his sequencing reaction (he did include ddATP, ddCTP, and ddTTP along with all the other needed components).
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A.All DNA fragments will terminate at the first G base pair
B.A color will be missing from the chromatogram.
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Chapter 7 Solutions
Genetic Analysis: An Integrated Approach (2nd Edition)
Ch. 7 - What results from the experiments of Frederick...Ch. 7 - 7.2 Explain why Avery, MacLeod, and McCarty’s in...Ch. 7 - 7.3 Hershey and Chase selected the bacteriophage...Ch. 7 - 7.4 Explain how the Hershey and Chase experiment...Ch. 7 - 7.5 One strand of a fragment of duplex DNA has the...Ch. 7 - 7.6 The principles of complementary base pairing...Ch. 7 - For the following fragment of DNA, determine the...Ch. 7 - 7.8 Figures present simplified depictions of...Ch. 7 - 7.9 Consider the sequence -ACGCTACGTC-.
What is...Ch. 7 - DNA polymerase III is the main DNA-synthesizing...
Ch. 7 - Explain how RNA participates in DNA replication.Ch. 7 - A sample of double-stranded DNA is found to...Ch. 7 - Bacterial DNA polymerase I and DNA polymerase III...Ch. 7 - Diagram a replication fork in bacterial DNA and...Ch. 7 - Prob. 16PCh. 7 - Which of the following equalities is not true for...Ch. 7 - List the order in which the following proteins and...Ch. 7 - Two viral genomes are sequenced, and the following...Ch. 7 - Matthew Meselson and Franklin Stahl demonstrated...Ch. 7 - Raymond Rodriguez and colleagues demonstrated...Ch. 7 - 7.22 Joel Huberman and Arthur Riggs used pulse...Ch. 7 - 7.23 Why do the genomes of eukaryotes, such as...Ch. 7 - Bloom syndrome (OMIM 210900) is an autosomal...Ch. 7 - 7.25 How does rolling circle replication (see...Ch. 7 - Telomeres are found at the ends of eukaryotic...Ch. 7 - A family consisting of a mother (I-1), a father...Ch. 7 - In a dideoxy DNA sequencing experiment, four...Ch. 7 - Prob. 29PCh. 7 - Using an illustration style and labeling similar...Ch. 7 - A PCR reaction begins with one double-stranded...Ch. 7 - Prob. 32PCh. 7 - Three independently assorting VNTR markers are...Ch. 7 - 7.34 A sufficient amount of a small DNA fragment...Ch. 7 - Prob. 35P
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Which of the following results is he most likely to observe? a. The reaction will work, but at a significantly slower rate. b. The reaction will work, but the product will contain many undesired mutations. c. The reaction will work, but amplify a region that was not his target. d. The reaction will be completely unsuccessfuarrow_forward(A) After three cycles of PCR, how many DNA molecules are present that correspond precisely to the desired amplification product? (B) What about after 5 cycles. Assume that we started with one molecule in each case, and that the reaction is perfectly efficient.arrow_forwardWhen joining two or more DNA fragments, a researcher can adjust the sequence at the junction in a variety of subtle ways, as seen in the following exercises.(a) Draw the structure of each end of a linear DNA fragment produced by an EcoRI restriction digest (include those sequences remaining from the EcoRI recognition sequence).(b) Draw the structure resulting from the reaction of this end sequence with DNA polymerase I and the four deoxynucleoside triphosphates.(c) Draw the sequence produced at the junction that arises if two ends with the structure derived in (b) are ligated (d) Draw the structure produced if the structure derived in (a) is treated with a nuclease that degrades only single-stranded DNA.(e) Draw the sequence of the junction produced if an end with structure (b) is ligated to an end with structure (d).(f) Draw the structure of the end of a linear DNA fragment that was produced by a PvuII restriction digest (include those sequences remaining from the PvuII recognition…arrow_forward
- You are working in a molecular biology laboratory and are having challenges with your PCR. You decide to double-check the PCR protocol programmed into the thermal cycler and discover that the annealing temperature was programmed to be 65 °C instead of 50 °C, as you had intended. What effects would this mistake have on the PCR reaction? Suggest potential solution(s).arrow_forwardYou are performing a PCR reaction but unbeknownst to you, there is a significant pool of dUTP in the nucleotide mix (along with dCTP, dTTP, dATP, and dGTP). How might this affect your PCR product? a. If the PCR product was ligated into a plasmid and put into a cell, a totally different mRNA would be made from the insert compared to an insert made with T's. b. If the pool of dTTP ran out before the pool of dUTP, DNA replication could no longer occur. c. During the reaction, uracils incorporated into the product would cause the PCR product to degrade as it is being made. d. Uracil would be incorporated into the product and would lessen the affinity of any DNA binding proteins that might bind to the product in subsequent experiments. e. Nothing would happen since polymerases can't use dUTP to make DNA.arrow_forwardA small DNA molecule was cleaved with several different restriction nucleases, and the size of each fragment was determined by gel electrophoresis.The following data were obtained. (a) Is the original molecule linear or circular?(b) Draw a map of restriction sites (showing distances between sites) that isconsistent with the data given.(c) How many additional maps are compatible with the data?(d) What would have to be done to locate the cleavage sites unambiguouslywith respect to each other?arrow_forward
- Running a PCR Why is a Hotstart necessary and how is it achieved? Why are the three temperatures necessary for PCR? Explain the role of each. Why does the lid of the thermocycler need to be heated? Why did we conduct an amplification reaction with no DNA? When looking at your PCR results why do you only see one band? What size were each of your PCR products?arrow_forwardIn PCr amplification If your negative control would have shown a different bp band that what was expected, what can be a possible explanation?arrow_forward. In the PCR process, if we assume that each cycle takes5 minutes, how manyfold amplification would be accomplished in 1 hour?arrow_forward
- In PCR amplification Why is it important to know the length of the sequence you amplify?arrow_forwardIn a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.arrow_forwarda）What two restriction sites are you going to use to clone your PCR product into the pL4440 plasmid? What are their DNA sequence? b) State the primer sequence you will use to amplify the F27C1.7 gene ready to be cloned into the pL4440 plasmid? c) How would you go about cloning this amplified DNA into pL4440? Using your knowledge of cloning list 5 important aspects of the method.arrow_forward
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