Brock Biology of Microorganisms, Books a la Carte Edition (14th Edition)
14th Edition
ISBN: 9780321928351
Author: MADIGAN, Michael T., Martinko, John M., Brock, Thomas, Bender, Kelly S., BUCKLEY, Daniel H., Stahl, David A.
Publisher: PEARSON
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Chapter 7, Problem 5RQ
Summary Introduction
The lac operon is used to synthesize the β-galactosidase to
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Describe the mechanism by which cAMP receptorprotein (CRP), the regulatory protein for catabolite repression, functions. Use the lactose operon as an example.
What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? (Explain what would happen both in the presence and absence of lactose)
Explain why inactivation of the O1 sequence of the lac operator almost completely abolishes repression of the lac operon.
Chapter 7 Solutions
Brock Biology of Microorganisms, Books a la Carte Edition (14th Edition)
Ch. 7.1 - Prob. 1MQCh. 7.1 - Prob. 2MQCh. 7.1 - Prob. 3MQCh. 7.2 - What is protein domain?Ch. 7.2 - Why are most DMA-binding proteins specific to...Ch. 7.3 - Why is negative control so named?Ch. 7.3 - How does a repressor inhibit the synthesis of a...Ch. 7.4 - Prob. 1MQCh. 7.4 - Prob. 2MQCh. 7.5 - Prob. 1MQ
Ch. 7.5 - Prob. 2MQCh. 7.5 - Explain how the lac operon is both positively and...Ch. 7.6 - What is the major difference between...Ch. 7.6 - How do transcriptional activators in Archaea often...Ch. 7.7 - What are kinases and what is their role in...Ch. 7.7 - Prob. 2MQCh. 7.8 - What are the primary response regulator and the...Ch. 7.8 - Why is adaptation during chemotaxis important?Ch. 7.8 - How does the response of the chemortaxis system to...Ch. 7.9 - Prob. 1MQCh. 7.9 - Prob. 2MQCh. 7.10 - Prob. 1MQCh. 7.10 - Prob. 2MQCh. 7.10 - Prob. 3MQCh. 7.11 - How are different sets of genes expressed in the...Ch. 7.11 - Prob. 2MQCh. 7.12 - Why are the levels of DnaA protein controlled...Ch. 7.12 - Prob. 2MQCh. 7.14 - Prob. 1MQCh. 7.14 - Prob. 2MQCh. 7.15 - What happens when a riboswitch binds the small...Ch. 7.15 - What are the major differences between a repressor...Ch. 7.16 - Why does attenuation control not occur in...Ch. 7.16 - Prob. 2MQCh. 7.17 - What is feedback inhibition?Ch. 7.17 - Prob. 2MQCh. 7.18 - Prob. 1MQCh. 7.18 - Prob. 2MQCh. 7 - Prob. 1RQCh. 7 - Describe why a protein that binds to a specific...Ch. 7 - REVIEW QUESTIONS
3. Most biosynthetic operons need...Ch. 7 - What is the difference between an operon and a...Ch. 7 - Prob. 5RQCh. 7 - Prob. 6RQCh. 7 - Prob. 7RQCh. 7 - Adaptation allows the mechanism controlling...Ch. 7 - How can quorum sensing be considered a regulatory...Ch. 7 - Prob. 10RQCh. 7 - Prob. 11RQCh. 7 - Prob. 12RQCh. 7 - Prob. 13RQCh. 7 - Prob. 14RQCh. 7 - Prob. 15RQCh. 7 - Prob. 16RQCh. 7 - Prob. 17RQCh. 7 - Application Questions
1. What would happen to...Ch. 7 - Prob. 2AQCh. 7 - Prob. 3AQCh. 7 - Most of the regulatory systems described in this...Ch. 7 - Many amino acid biosynthetic operons under...Ch. 7 - How would you design a regulatory system to make...
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- A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcriptionarrow_forwardINTERPRET DATA Develop a simple hypothesis that would explain the behavior of each of the following types of mutants in E. coli. Mutant a: The map position of this mutation is in the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if large amounts of tryptophan are present in the growth medium. Mutant b: The map position of this mutation is in the trp operon. The mutant cells do not produce any enzymes coded for by the trp operon under any conditions. Mutant c: The map position of this mutation is some distance from the trp operon. The mutant cells are constitutive; that is, they produce all the enzymes coded for by the trp operon, even if the growth medium contains large amounts of tryptophan.arrow_forwardDescribe how the components of the trp operon system are affected by a mutation in trpC? Why is it that once enviornmental tryptophan is inserted into the cell, only the trp repressor is activated (turned on)?arrow_forward
- If an abnormal repressor protein could still bind allolactose but the binding of allolactose did not alter the conformation of the repressor protein, how would the expression of the lac operon be affected?arrow_forwardWhat would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? (Explain what would happen in both the presence and absence of tryptophan)arrow_forwardDescribe the role of the CRP–cAMP complex in positiveregulation of the lactose operon in E. coli.arrow_forward
- In the lac operon (below), how will expression of the genes lacZ and lacy be effect by a mutation in the repressor protein (encoded by lach) that prevents it from binding the operator sequence (lacO) in the absence of lactose? Explain the answerarrow_forwardIf glucose levels in the cell are high and lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?arrow_forwardIf glucose is not available, but lactose is available from the environment, what is the status of transcription of the lac operon genes? Explain your answer from both an evolutionary perspective and in terms of negative and positive regulation of the operon?arrow_forward
- In terms of the trp operon, differentiate between two normal bacterial cultures, one grown in a medium supplied with tryptophan and the other medium without tryptophan.arrow_forwardWhen cAMP binds to the Catabolite Activator Protein (CAP), it causes a conformational change on the protein, which results in binding to the CAP-binding site on the DNA and ___________________________ of the lac operon. This conformational change induced by the binding of another molecule is known as a(n) _________________________________. transcription repression; binding effect translation; distortion effect mutation; interactive effect attenuation; compression effect transcription activation; allosteric effectarrow_forwardWhich of the following diagrams best models the regulation of the trp operon when tryptophan is present at high levels?arrow_forward
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