BIOLOGY (LL)
BIOLOGY (LL)
5th Edition
ISBN: 9781264115495
Author: BROOKER
Publisher: MCG
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Chapter 7, Problem 6TY
Summary Introduction

Introduction: Racker and Stoeckenicus conducted an experiment in which the orientation of bacteriorhodopsin was made such that H+ was pumped into a vesicle and each vesicle contains many molecules of bacteriorhodopsin.

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The following table depicts the rate of transport of a molecule into a cell as the external concentrations are changed. Based on what you know about the characteristics of membrane transport, the molecule is most likely transported by: a) passive transport b) simple diffusion across the membrane c) facilitated diffusion d) active transport
To study the mechanism of SERCA, you prepare membrane vesicles containing this protein oriented such that its ATP binding site is on the outer surface of the vesicle. To measure pump activity, you use an assay that detects the formation of inorganic phosphate in the medium. When you add calcium and ATP to the medium, you observe phosphate production for only a short period of time. Only after the addition of calcimycin , a molecule that makes membranes selectively permeable to calcium, do you observe sustained phosphate production. Explain.
If you start with 100 mM Na+ and 100 mM K+ outside the liposomes, and 100 mM Na+ and 100 mM K+ inside the liposomes like in (A), then you add ATP to the solution outside the liposomes, then how will the Na+ and K+ concentrations change?
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