Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Chapter 8, Problem 2R
DRAW IT Identify and mark each of the following on the portion of
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Arrange the events of replication chronologically from A (first) to G (last event):
-Sealing of phosphodiester linkage
-Replacement of RNA primer with new DNA strand
-Unwinding of the double helix
-Addition of short RNA primers
-Recognition of origin of replication
-Synthesis of complementary DNA strand at the leading strand of the DNA
-Excision of short RNA primers
Circle the following elements that are directly involved in DNA replication process
DNA helicase RNA primase
Sliding clamp DNA polymerase
DNA ligase
Define the following words: replication fork, leading strand, lagging strand, Okazaki fragments, helicase, single strand, binding protein, primase, DNA polymerase 3, DNA polymerase 1, dna ligase and nuclease
Chapter 8 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 8 - Briefly describe the components of DNA, and...Ch. 8 - DRAW IT Identify and mark each of the following on...Ch. 8 - Match the following examples of mutagens. Column A...Ch. 8 - The following is a code for a strand of DNA. a....Ch. 8 - Prob. 5RCh. 8 - Identify when (before transcription, after...Ch. 8 - Which sequence is the best target for damage by UV...Ch. 8 - You are provided with cultures with the following...Ch. 8 - Why are mutation and recombination important in...Ch. 8 - NAME IT Normally a commensal in the human...
Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Feedback inhibition differs from repression...Ch. 8 - Bacteria can acquire antibiotic resistance by all...Ch. 8 - Suppose you inoculate three flasks of minimal...Ch. 8 - Plasmids differ from transposons in that plasmids...Ch. 8 - Mechanism by which the presence of glucose...Ch. 8 - The mechanism by which lactose controls the lac...Ch. 8 - Two offspring cells are most likely to inherit...Ch. 8 - Which of the following is not a method of...Ch. 8 - Nucleoside analogs and ionizing radiation are used...Ch. 8 - Replication of the E. coli chromosome takes 40 to...Ch. 8 - Pseudomonas has a plasmid containing the mer...Ch. 8 - Ciprofloxacin, erythromycin, and acyclovir are...Ch. 8 - HIV, the virus that causes AIDS, was isolated from...Ch. 8 - Human herpesvirus-8 (HHV-8) is common in parts of...
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- Describe the process using the terms provided. DNA replication: 3' end, 5' end, DNA polymerase 1, DNA polymerase 3, helicase, lagging strand, leading strand, ligase, primase, single-stranded binding proteins, topoisomerasearrow_forwardWhich of the following statements about DNA replication is FALSE? Select one: Unwinding of the DNA molecule occurs as hydrogen bonds break. The new strand of DNA is synthesized from 5’ to 3’ direction. Replication occurs as each base is paired with the same base. In eukaryotes, replication can occur at numerous replication bubbles, each with two forks.arrow_forwardDetermine the complementary strand of DNA that forms on this template DNA fragment during replication: 5′GGTTTCTTCAAGAGA3′arrow_forward
- Which of the following statements are TRUE?I. DNA replication is a semiconservative process wherein the two resulting double helices consist of one new strand and one parental strand.II. The DNA strand that is used to make a complementary daughter strand is called the parental strand.III. The precursor of each new nucleotide in the DNA strand is a deoxynucleoside 3′-triphosphate.IV. The incoming nucleotide always attaches to 5′-phosphate of the previously added nucleotidearrow_forwardWhich of the following statements are TRUE?I. DNA replication is a semiconservative process wherein the two resulting double helices consist of one new strand and one parental strand.II. The DNA strand that is used to make a complementary daughter strand is called the parental strand.III. The precursor of each new nucleotide in the DNA strand is a deoxynucleoside 3′-triphosphate.IV. The incoming nucleotide always attaches to 5′-phosphate of the previously added nucleotide a. I only b. II only c. I and IV d. III and IVarrow_forwardWhich of the following replication enzymes will most likely be involved in COVID19 RNA replication? a) Helicase, Polymerase, Ligase b) SSB protein, Polymerase, Ligase c) Topoisomerase, Primase, Ligase d) Helicase, SSB protein, Primase e) Topoisomerase, Polymerase, Primasearrow_forward
- Which of the following most correctly describes a process that occurs during DNA replication? Group of answer choices Replication of the lagging strand occurs in the 5' → 3' direction—the leading strand in the 3' → 5' direction. Replication is continuous on the lagging strand and discontinuous on the leading strand. Okazaki fragments are DNA fragments synthesized on the lagging strand. DNA polymerase adds dNTP monomers in the 3′–5′ direction. Replication is continuous on the lagging strand and discontinuous on the leading strand.arrow_forwardDraw a replication bubble with both replication forks and label the origin of replication, the leading strands, lagging strands, and the 5' and 3' ends of all strands shown in your diagram. Pls help asap!arrow_forwardDraw a diagram of a single replication fork. Label the following on your diagram: 1. All 3’-OH ends 2. All 5’-phosphate ends 3. leading strand (s) 4. lagging strand (s) 5. Okazaki fragments (draw 3) ❏ RNA primer(s) 6. direction of replication ❏ replisome(s) 7. origin (s) 8. terminus (i)arrow_forward
- Which of the following statements about replication is false? The proofreading function of DNA polymerases involves 3’ 5’ exonuclease activity. Origins of replication are rich in G-C nucleotide pairs. A characteristic of aging cells is that telomeres become shorter. The lagging strand is synthesized in the opposite direction as the movement of the replication fork. DNA polymerase is more accurate that RNA polymerase.arrow_forwardWhich of the following statements BEST describes the process of DNA Replication? Group of answer choices The two strands of the parental molecule separate and serves as template for synthesis of a new strand. Each new strand is identical to its template strand. Each strand of both daughter molecules contains a mixture of old and newly synthesized DNA. The two parental strands reassociate after acting as templates for new strands restoring the parental double helix.arrow_forwardA new DNA strand elongates only in the 5' to 3' direction because _____. replication must progress toward the replication fork DNA polymerase begins adding nucleotides at the 5' end of the template DNA polymerase can add nucleotides only to the free 3' end the polarity of the DNA molecule prevents addition of nucleotides at the 3' endarrow_forward
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