(a)
Interpretation:
The peptide that can be digested most rapidly and most slowly should be determined.
Concept introduction:
KM is the Michaelis constant which is the substrate concentration at the half of the Vmax. k2 is also referred as kcat which is known as turn over number. The turnover number is defined as the number of substrate molecules converted into product per unit time at a single catalytic site. The ratio between kcat/KM is a measure of enzyme efficiency and specificity for a common substrate.
(b)
Interpretation:
The features of the amino acid sequence describing the specificity of the enzyme should be suggested.
Concept introduction:
KM is the Michaelis constant which is the substrate concentration at the half of the Vmax. k2 is also referred as kcat which is known as turn over number. Turn over number is defined as the number of substrate molecules converted into product per unit time at a single catalytic site. The ratio between kcat/KM is a measure of enzyme efficiency and specificity for a common substrate.
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