(a)
Interpretation:
V0 versus pH curve when the substrate concentration is much greater than the enzyme KM should be drawn.
Concept introduction:
KM is the Michaelis constant which is the substrate concentration when the reaction rate is half of the maximum rate achieved by a system. Michaelis constant is a measure of its binding affinity to a substrate. The higher the Michaelis constant, the lower affinity for its substrate. That means an enzyme with lower KM needs a higher concentration of substrate to achieve Vmax.
(b)
Interpretation:
V0 versus pH curve when the substrate concentration is much less than the enzyme KM should be drawn.
Concept introduction:
KM is the Michaelis constant which is the substrate concentration when the reaction rate is half of the maximum rate achieved by a system. Michaelis constant is a measure of its binding affinity to a substrate. The higher the Michaelis constant, lower affinity for its substrate. That means an enzyme with lower KM needs a higher concentration of substrate to achieve Vmax.
(c)
Interpretation:
The pH at which the velocity will equal half of the maximal velocity attainable under these conditions should be determined.
Concept introduction:
KM is the Michaelis constant which is the substrate concentration when the reaction rate is half of the maximum rate achieved by a system. Michaelis constant is a measure of its binding affinity to a substrate. The higher the Michaelis constant, lower affinity for its substrate. That means for an enzyme with lower KM needs a higher concentration of substrate to achieve Vmax.
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- Hand written solutions are strictly prohibited.arrow_forwardpls explainWhy was absorbance measured at 420 nm in the enzyme kinetics experiment? To monitor thea. decrease in catechol concentrationb. increase in quinone concentrationc. decrease in polyphenol oxidase concentrationd. polyphenol oxidase-catechol complex concentrationLineweaver-Burk plot is the most used linearization method of the MichaelisMenten equation, but the main drawback is a/an:a. overemphasis to low substrate concentration and less emphasis to high substrate concentration.b. overemphasis to high substrate concentration and less emphasis to low substrate concentration.c. exaggeration to both ends of the reciprocal of the substrate concentration.d. lack of independent variables to x- and y- coordinatesarrow_forwardHiI have this question, Can you help me?arrow_forward
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