MICROBIOLOGY-ACCESS >CUSTOM<
13th Edition
ISBN: 9780135668825
Author: Tortora
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 8MCQ
The mechanism by which lactose controls the lac operon.
Use the following choices to answer questions 7 and 8.
- a. catabolite repression
- b. DNA polymerase
- c. induction
- d. repression
- e. translation
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Answer the following questions:
1. Given the following genotypes, explain how the mutation (identified by a () superscript will affect E.
coli grown in lactose medium. Will the lac operon be on or off? Will there be a complete set of gene
products from the loc operon? What will be the implication of the missing gene product, if ever? Will the
cell be able to survive in the lactose medium or not?
a. p' oE Y
b. p'o Y
d. po v
he bands indicated by arrow "A" (to the left of the western blot) most likely represent:
A.
PX-HA lacking any post-translational modifications.
B.
dephosphorylated PX-HA.
C.
phosphorylated PX-HA.
D.
PX-HA with its N-terminal signal sequence still attached.
E.
glycosylated PX-HA.
Define the following terms: a. promoter b. consensus sequence c. operon d. chromatin-remodeling complex e. general transcription factors
Chapter 8 Solutions
MICROBIOLOGY-ACCESS >CUSTOM<
Ch. 8 - Briefly describe the components of DNA, and...Ch. 8 - DRAW IT Identify and mark each of the following on...Ch. 8 - Match the following examples of mutagens. Column A...Ch. 8 - The following is a code for a strand of DNA. a....Ch. 8 - Prob. 5RCh. 8 - Identify when (before transcription, after...Ch. 8 - Which sequence is the best target for damage by UV...Ch. 8 - You are provided with cultures with the following...Ch. 8 - Why are mutation and recombination important in...Ch. 8 - NAME IT Normally a commensal in the human...
Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Match the following terms to the definitions in...Ch. 8 - Feedback inhibition differs from repression...Ch. 8 - Bacteria can acquire antibiotic resistance by all...Ch. 8 - Suppose you inoculate three flasks of minimal...Ch. 8 - Plasmids differ from transposons in that plasmids...Ch. 8 - Mechanism by which the presence of glucose...Ch. 8 - The mechanism by which lactose controls the lac...Ch. 8 - Two offspring cells are most likely to inherit...Ch. 8 - Which of the following is not a method of...Ch. 8 - Nucleoside analogs and ionizing radiation are used...Ch. 8 - Replication of the E. coli chromosome takes 40 to...Ch. 8 - Pseudomonas has a plasmid containing the mer...Ch. 8 - Ciprofloxacin, erythromycin, and acyclovir are...Ch. 8 - HIV, the virus that causes AIDS, was isolated from...Ch. 8 - Human herpesvirus-8 (HHV-8) is common in parts of...
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- A mutation that inactivates the repressor gene of the lac operon results in (a) the continuous transcription of the structural genes (b) no transcription of the structural genes (c) the binding of the repressor to the operator (d) no production of RNA polymerase (e) no difference in the rate of transcriptionarrow_forwardDecide which operon each of the following characteristics applies to. Note: a description may apply to both. a. lac operon b. trp operon 68. repressible 69. Turned off when end product is present (feedback inhibition) 70. Promoter, activator region, and 3 genes 71. Repressible 72. contain operator and promoter regions as well as transcript units of at least two or more coding genes 73. Codes for catabolic enzymes that break down lactose 74. Inducible 75. Promoter, activator region, and 5 genes 76. Codes for anabolic enzymes that help manufacture tryptophan 77. Lactose is the co-inducer 78. Turned on when substrate is present 79. Tryptophan is the co-repressor 80. Regulatory gene segmentsarrow_forwardImagine this scenario: In E.coli the Operon X encodes for Proteins Y, X and Z. Proteins Y, X and Z are required for growth in the presence of Drug A. Outline the experiments that you would conduct to assess whether Protein B represses the transcription of Operon X in the absence of Drug A. Things to consider: 1. Transcription of Operon X 2. Mutations 3. Chromatin structurearrow_forward
- Suppose that E. coli sustains a mutation in its gene for the lac operon repressor making the repressor ineffective . How would this mutation affect the bacterium's ability to catabolize lactose? Would the mutant strain have an advantage over the wild-type strain? Explain your answer.arrow_forwardExamine the following theoretical operon (gen) in E. coli which contains structural genes (genC, genB, genA) that are involved in amino acid biosynthesis. The gen operon also includes the gene encoding for the regulator molecule within it. When the amino acid that this operon helps synthesize is present, it binds to the regulator forming a complex which can bind to the operator region. Assume that the regulator molecule only binds to the operator in the presence of the amino acid. Promoter || Operator genC genB gen operon Regulator genAarrow_forwardSuppose that E. coli sustains a mutation in its gene for the lac operon repressor such that the repressor is ineffective. What effect would this have on the bacterium’s ability to catabolize lactose? Would the mutant strain have an advantage over wild-type cells? Explain your answer.arrow_forward
- Which statement about the transcription attenuation mechanism is TRUE? 1.In some operons (e.g., the his operon), attenuation may be the only regulatory mechanism. 2. Sequences of the trp operon leader RNA resemble an operator. 3. The leader peptide acts by a mechanism that is similar to that of a repressor protein. 4.The leader peptide gene of the trp operon includes no Trp codons. 5. The leader peptide is an enzyme that catalyzes transcription attenuation.arrow_forwardDefine the following in the context of operons: a. positive control b. give an example of an operon that is under positive control c. negative control d. give an example of an operon that is under negative control e. repressor f. give an example of a repressor molecule g. activator h. give an example of an activatorarrow_forwardPlease complete the table representing the regulation of the lactose operon. Please highlight any data that provides evidence of trans regulation.arrow_forward
- Below is an electron micrograph illustrating the process of simultaneous transcription and translation in a prokaryotic cell. Use this illustration to answer the question. A. Which of the following statements is correct? 1. The direction indicated by A, is the correct direction of RNA polymerase movement. 2. If C and D are both pointing to the first ribosome in the chain, then ribosome C is carrying a longer polypeptide chain. 3. If E is pointing to mRNA, then it is pointing to the 5'end of the mRNA. 4. If B is pointing to the DNA, then it is pointing to the 5' end of the DNA. O A. 1, 2 and 3 O B. 1 and 3 O C. 2 and4 O D. 4 only O E. All of 1, 2, 3 and 4 are correctarrow_forwardA researcher was trying to determine whether two molecules(A nd B) were corepressors or inducers in their respective operon systems. Data was collected regarding the levels of protein and the amount of gene transcription for the genes in theri respectiver operons. The data is shown below. Level of protein Transcription of gene 1 low Transcription of gene 2 Molecule A High low Low high high Molecule B High high high Low low lowarrow_forwardIn the trp operon, tryptophan is best characterized as being a: A. repressor B. inducer C. corepressor D. DNA-binding domainarrow_forward
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