Fundamentals of Biochemistry: Life at the Molecular Level
Fundamentals of Biochemistry: Life at the Molecular Level
5th Edition
ISBN: 9781118918401
Author: Donald Voet, Judith G. Voet, Charlotte W. Pratt
Publisher: WILEY
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Chapter 9, Problem 29CQ

(a)

Summary Introduction

To describe: The labeling pattern of glycophorin A, when a membrane-impermeable protein labeling reagent is added to a preparation of solubilized erythrocyte proteins.

Concept introduction: Glycophorin is a sialoglycoprotein in the red blood cell membrane. Glycophorins are rich in sialic acid and it carries sugar molecules. It is a membrane spanning protein which is heavily glycosylated. The presence of sialic acid gives the red blood cell a hydrophilic charged coat. Glycophorin A also known as GYPA belongs to the family of glycophorins.

(b)

Summary Introduction

To describe: The labeling pattern of glycophorin A when a membrane-impermeable protein labeling reagent is added to the intact erythrocyte ghosts.

Concept introduction: Glycophorin is a sialoglycoprotein in the red blood cell membrane. Glycophorins are rich in the sialic acid and it carries sugar molecules. It is a membrane spanning protein which is heavily glycosylated. The presence of sialic acid gives the red blood cell a hydrophilic charged coat. Glycophorin A also known as GYPA belongs to the family of glycophorins.

(c)

Summary Introduction

To describe: The labeling pattern of glycophorin A, when a membrane-impermeable protein labeling reagent is added to an erythrocyte ghosts that are initially leaky and then immediately sealed and transferred to a solution that does not contain the labeling reagent.

Concept introduction: Glycophorin is a sialoglycoprotein in the red blood cell membrane. Glycophorins are rich in the sialic acid and it carries sugar molecules. It is a membrane spanning protein which is heavily glycosylated. The presence of sialic acid gives the red blood cell a hydrophilic charged coat. Glycophorin A also known as GYPA belongs to the family of glycophorins.

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Fundamentals of Biochemistry: Life at the Molecular Level

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Macromolecules | Classes and Functions; Author: 2 Minute Classroom;https://www.youtube.com/watch?v=V5hhrDFo8Vk;License: Standard youtube license