Nester's Microbiology: A Human Perspective
9th Edition
ISBN: 9781259709999
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Question
Chapter 9, Problem 2CT
Summary Introduction
To review:
Development of DNA (deoxyribonucleic acid) probe based on amino acid sequenceis tootime consuming.
Introduction:
DNA probe is a
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Students have asked these similar questions
An effective DNA probe can sometimes be developed by knowing the amino acid sequence of the protein encoded by the gene. A student argued that this is too time-consuming since the complete amino acid sequence must be determined in order to create the probe. Does the student have a valid argument? Why or why not?
For what purposes is DNA extraction done? (give at least 3 purposes for which you may need to extract DNA)
From your knowledge about DNA microarray, answer the following:
Why RT-PCR is important in the sample preparation to perform expression microarray experiment?(
Chapter 9 Solutions
Nester's Microbiology: A Human Perspective
Ch. 9 -
1. Why are restriction enzymes useful in...Ch. 9 -
2. In the CRISPR/Cas9 system, how is the Cas9...Ch. 9 -
3. Describe three general uses of genetically...Ch. 9 - Describe three uses of transgenic plants.Ch. 9 - Prob. 5SACh. 9 -
6. What is cDNA? Why is it used when cloning...Ch. 9 - How many different temperatures are used in each...Ch. 9 -
8. How does PCR eventually generate a...Ch. 9 -
9. What is the function of a DNA probe?
Ch. 9 -
10. How does a DNA microarray function as a set...
Ch. 9 - What is the function of a vector? a) Destroys...Ch. 9 -
2. The Ti plasmid of Agrobacterium tumefaciens is...Ch. 9 - Prob. 3MCCh. 9 - An ideal vector has all of the following except a)...Ch. 9 - Prob. 5MCCh. 9 - Prob. 6MCCh. 9 - Prob. 7MCCh. 9 - Prob. 8MCCh. 9 - Prob. 9MCCh. 9 - The polymerase chain reaction generates a fragment...Ch. 9 -
1. Two students in a microbiology class are...Ch. 9 - Prob. 2ACh. 9 - Discuss some potential issues regarding gene...Ch. 9 - Prob. 2CT
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Similar questions
- Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.arrow_forwardCloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardThe following question is related to Restriction Enzymes and RFLP. Using EcoRl, which DNA sequence held the largest size fragment? DNA sequence A or B? arrow_forward
- If you worked backward, starting with the amino acid sequence of the protein, would you obtain the same DNA nucleotide sequence? Why or why not?arrow_forwardAbove are the results of gel electrophoresis following digestion with restriction enzymes. What is the total length of the DNA fragment? Which enzyme, HindIII or EcoRI, produced a larger fragment? What part of DNA causes it to be negatively charged in order for electrophoresis to work?arrow_forwardRestriction enzymes look for palindromic sequences of DNA to cut, how does it recognize those sequences? Does it bind to them and read their strands? Does it work with both strands of the DNA or does it just need one strand to work its process?arrow_forward
- Why do some journals require the authors of articles describing DNA libraries to make those libraries available to other researchers?arrow_forwardMost PCR reactions do not use the more expensive types of DNA polymerase, which have DNA proofreading. How might this be a problem in accurately copying specific DNA sequences of the target gene?arrow_forwardThe last steps of DNA extraction include washing it using ethyl alcohol and drying the DNA pellet. Why do we need to wash the DNA using ice-cold ethyl alcohol? And why do we have to dry the DNA pellet?arrow_forward
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