Essentials of Genetics (9th Edition) - Standalone book
9th Edition
ISBN: 9780134047799
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Chapter 9, Problem 32PDQ
During electrophoresis, DNA molecules can easily be separated according to size because all DNA molecules have the same charge–mass ratio and the same shape (long rod). Would you expect RNA molecules to behave in the same manner as DNA during electrophoresis? Why or why not?
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During gel electrophoresis, DNA molecules can easily be separated according to size because all DNA molecules have the same charge-to-mass ratio and the same shape (long rod). Would you expect RNA molecules to behave in the same manner as DNA during gel electrophoresis? Why or why not?
What feature of a DNA fragment causes it to move through a gel during electrophoresis? a. the electrical charges of its phosphate groups b. its nucleotide sequence c. the hydrogen bonds between its base pairs d. its double helix shape
Suppose a piece of DNA is cut into four fragments of varying sizes. Where would you expect to find the larger fragments on the gel (top, middle, or bottom)? Why?
Suppose you had 1000 pieces of each of the four fragments, how many bands would appear on the gel?
Chapter 9 Solutions
Essentials of Genetics (9th Edition) - Standalone book
Ch. 9 - CASE STUDY |Zigs and zags of the smallpox virus...Ch. 9 -
CASE STUDY | Zigs and zags of the smallpox...Ch. 9 - CASE STUDY | Zigs and zags of the smallpox...Ch. 9 - CASE STUDY | Zigs and zags of the smallpox virus...Ch. 9 -
HOW DO WE KNOW?
1. In this chapter, we have...Ch. 9 - Review the Chapter Concepts list on p. 160. Most...Ch. 9 - Discuss the reasons why proteins were generally...Ch. 9 -
4. Contrast the various contributions made to our...Ch. 9 - When Avery and his colleagues had obtained what...Ch. 9 - Why were 32P and 35S chosen in the Hershey–Chase...
Ch. 9 - Does the design of the Hershey-Chase experiment...Ch. 9 - What observations are consistent with the...Ch. 9 - What are the exceptions to the general rule that...Ch. 9 -
10. Draw the chemical structure of the three...Ch. 9 - How are the carbon and nitrogen atoms of the...Ch. 9 - Adenine may also be named 6–amino purine. How...Ch. 9 -
13. Draw the chemical structure of a dinucleotide...Ch. 9 - Describe the various characteristics of the...Ch. 9 - Prob. 15PDQCh. 9 - What might Watson and Crick have concluded, had...Ch. 9 - Prob. 17PDQCh. 9 - Prob. 18PDQCh. 9 - Prob. 19PDQCh. 9 - Prob. 20PDQCh. 9 - Prob. 21PDQCh. 9 - Prob. 22PDQCh. 9 -
23. Why is Tm related to base composition?
Ch. 9 - What is the chemical basis of molecular...Ch. 9 - What did the Watson–Crick model suggest about the...Ch. 9 - A genetics student was asked to draw the chemical...Ch. 9 - Prob. 27PDQCh. 9 -
28. One of the most common spontaneous lesions...Ch. 9 - Prob. 29PDQCh. 9 - Prob. 30PDQCh. 9 - Prob. 31PDQCh. 9 -
32. During electrophoresis, DNA molecules can...Ch. 9 - Assume that you are interested in separating short...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- DNA contains many hydrogen bonds. Are hydrogen bonds stronger or weaker than covalent bonds? What are the consequences of this difference in strength?arrow_forwardWhen DNA is heated sufficiently, the strands separate. The energy that it takes to separate the DNA is related to the amount of guanine and cytosine bases. Why is this so? (A) Adenine and thymine make 3 hydrogen bonds between each other, and guanine and cytosine don't make any hydrogen bonds. (B) Guanine and cytosine make 3 hydrogen bonds between each other, and adenine and thymine don't make any hydrogen bonds. (C) It takes more energy to break an A-T bond than it does a G-C bond. (D) It takes more energy to break a G-C bond than it does an A-T bond.arrow_forwardDuring electrophoresis, DNA molecules can easily be separatedaccording to size because all DNA molecules have the samecharge–mass ratio and the same shape (long rod). Would youexpect RNA molecules to behave in the same manner as DNAduring electrophoresis? Why or why not?arrow_forward
- In general, which part of a DNA sequence will have the best quality? (Ex. Beginning, middle, end?) Why?arrow_forwardDNA solution is viscous because of the nature of chemical substance that can intercalate into the DNA helix. An example of such substance is acridine orange. experiments revealed that acridine orange causes an increase in the viscosity of DNA solution.how would you account for this effect?arrow_forwardDNA fragments that are 500 bp, 1000 bp, and 2000 bp in length are separated by gel electrophoresis. Which fragment will migrate farthest in the gel? a. The 2000-bp fragment b. The 1000-bp fragment c. The 500-bp fragment d. All will migrate equal distances.arrow_forward
- Would it be easier or harder to fold DNA into a nucleosome at pH 1?arrow_forwardFrom an extract of human cell growing in tissue culture, A fibrous substance was obtained. How would you distinguish whether it was DNA or RNA?arrow_forwardSuppose you had a method of cutting DNA at specific sequences of nucleotides. how many nucleotides long (on average) would such a sequence have to be in order to make just one cut in a bacterial genome of 3 × 106 nucleotide pairs?arrow_forward
- Your lab partner tells you proteins are used to make enzymes, you say, no enzymes are used to make proteins. Who is right? Explain your answer. – realize that enzymes are proteins and that the enzyme polymerase is used to transcribe DNAarrow_forwardCompare a picture of a structure of Human DNA and Bacterial DNA, are they similar?arrow_forwardThe DNA polymerase cannot start making a new DNA polymer out of nothing, it needs preexisting nucleic acid to hook the new DNA onto. What is this preexisting nucleic acid called, what enzyme makes it, and what nucleic acid is the preexisting nucleic acid made of?arrow_forward
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