The effect that occurs in chymotrypsin when Ser 189 is changed to Asp 189 is to be predicted. Concept introduction: The organic compounds that contain amine ( -NH 2 ) and carboxyl ( -COOH ) functional groups with a side chain ( -R ) are known as amino acids. Amino acids consist of both positive as well negative groups in their structure and are called as ampholytes. The small chain of amino acids that is connected with the help of the amide bonds is known as peptide. A peptide possesses the covalent bonds which are formed by the reaction of carboxyl group of one amino acid with the amino group of other amino acids. Proteolytic enzymes commonly known as proteases are used for the cleavage of protein. Chymotrypsin is an enzyme that is used for the cleavage of protein through hydrolysis reaction.

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Inducers and Inhibitors of AEP. Short peptides such as legumain stabilization and activity modulation (LSAM) domain and αvβ3 integrin could enhance the activity of AEP. LSAM domain known as the prodomain of AEP blocks substrate binding before activation. This prodomain has a helical structure and two independent peptides. One is an activation peptide (AP, K287 to N323), and the other is a LSAM domain. LSAM domain remains even after AP is cleaved and released from protease at neutral pH via electrostatic interaction. AEP without LSAM domain has a lower melting temperature than AEP with LSAM domain [77, 117]. Another short peptide, αvβ3 integrin, can directly interact with AEP, and after forming a complex, the optimal pH for AEP activity is increased from 5.5 to 6.0. It indicates that αvβ3 binding could induce conformational stabilization of AEP accompanied by deprotonated C189. αvβ3 does not directly interact with the AEP active site; however, AEP docks to the αvβ3 RGD-binding site…

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Question

Chapter 9, Problem 4P

Interpretation Introduction

Interpretation:

The effect that occurs in chymotrypsin when Ser 189 is changed to Asp 189 is to be predicted.

Concept introduction:

The organic compounds that contain amine (-NH2) and carboxyl (-COOH) functional groups with a side chain (-R) are known as amino acids. Amino acids consist of both positive as well negative groups in their structure and are called as ampholytes. The small chain of amino acids that is connected with the help of the amide bonds is known as peptide. A peptide possesses the covalent bonds which are formed by the reaction of carboxyl group of one amino acid with the amino group of other amino acids.

Proteolytic enzymes commonly known as proteases are used for the cleavage of protein. Chymotrypsin is an enzyme that is used for the cleavage of protein through hydrolysis reaction.

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