Agar plate

potato dextrose agar (PDA). The PDA (10.26% potato extract, 51.28% glucose, 38.46% agar, 35.5366 Nm cycloheximide) uses potatoes as a source of nitrogen, potassium, and glucose as the carbon source, while cycloheximide is used to inhibit fungal growth. Of each dilution 0.1 ml was plated on PDA and spread with the hockey stick method. Each plate was then labelled with the dilution factor and placed in the incubator at 37°C for 24-48 hours dependent upon growth. A seventh plate was created with

properties 3.16.1. Blood hemolysis Hemolysis test was performed according to the method described by Guttmann and Ellar (2000). Overnight cultures of isolated Enterococcus durans and Enterococcus hirae were streaked on blood agar and incubated at 37°C for 24 h. Blood agar plates were examined for signs of hemolysis. Blood hemolysis test was performed in duplicates. 3.16.2. Resistance to low pH Isolates Enterococcus durans and Enterococcus hirae were tested for their ability to resist low pH values

gloves • Timer • Agar plates (you will need 12 of them.) • Notebook to record results Experimental Procedure: 1. Put on sterile gloves ( do not touch any non-sterile surfaces when wearing gloves) 2. Drop the first item of food (ham) on the first surface (tiles) 3. Start the timer 4. Remove the item from the surface after 5 seconds 5. Swab the item with a sterile swab (do not touch anything else with the swab) 6. Gently run the swab back and forth in a zigzag motion over the agar. Do not touch any

7mm in section one, 1.5mm in section two, and 0.5 in section three (table 1). The agar plate with a tap, sterilized water, had the largest microbial in size at about 1.7 mm. The light exposed water had an increase in size from section one at 0.5 mm to 1.1 in section 3. Analyzing each plate, there was a physical feature that was easily identify for example the surface, texture color and elevation. In the lighted plate, each section had a smooth surface appearance, section 1 had a rigid texture but

This report represents my individual effort. I did not receive or offer aid to anyone when performing this assignment, nor did I plagiarize any material. Signed: __________________________________________________________ Introduction Having clean water is a very crucial aspect of health. Many people don’t have access to clean water, this can be life threatening. There are lethal bacterial infections that are present in water. There are many different diseases that can acquire by drinking contaminated

Nutrient Agar To observe the colony morphology, the original unknown culture was streaked for isolation on nutrient agar using the quadrant streaking technique, inverted, and incubated at 37° C for 48 hours. This allowed for observation of the colony morphology. Separation of Gram-positive and Gram-negative The original unknown culture was streaked for isolation on Columbia CNA agar and MacConkey agar using a quadrant streak, inverted, and placed in a 37° C incubator for 48 hours. CNA agar contains

techniques, which will be used throughout this course, by aseptically transferring cultures to three types of media: broth, plate, and agar slant. Materials: • Lab coat • 10% bottle of bleach • Sharpie • Masking tape • Vortex mixer • Sterile Tryptic soy broth test tubes • Sterile Tryptic soy agar slants • Tryptic soy broth culture of Serratia marcescens • Tryptic soy agar slant culture of Serratia marcescens • Inoculating loop • Bunsen Burner • Striker Methods: Before you start any kind of Transfer

can be done is by running a culture and sensitivity test. Just keep in mind that there are many different types of culture medias and hundreds of different sensitivity discs that correlate to different drugs. A negative test on one of these media plates does not meant that there is nothing there. Which is why a lot of clinics, like our own, run them in house and also send them to a lab such as Idexx to have them run a more complete test there. A culture is a specific test to find and identify organisms

From the ancient times spices and herbs has been mainly used as natural food preservatives, as food additives and as flavouring agents. Various spices indicates antimicrobial action against distinctive sorts of microorganisms. This project proposal gives a literature review considering antimicrobial action of vital oils generally utilized as spices and herbs. For e.g.: garlic, mustard, bay, cumin, thyme, basil, oregano, pepper, ginger, sage, rosemary etc. Against most regular bacteria and fungi that

Lab Three: Streaking and Spreading Plates Introduction In this laboratory experiment, we was introduce to an introduction to streaking and spreading of bacteria in agar plates such that single cells can be isolated from one another, each cell can reproduces to form a visible colony composed of genetically identical clones. Streaking and spreading bacteria is to obtain individual colonies is usually the first step in genetic manipulation of microorganisms. Materials • Petri dishes • Metal transfer