Luciferase

Introduction Of the 70 percent of Earth that is covered in water, approximately 60 percent is deep ocean, which measures a depth of about 10,000 meters. The deep ocean is characterized by a hydrostatic pressure 400x atmospheric pressure at sea level and temperatures dropping well below 4 degrees Celsius (Danovaro et al. 2014). Furthermore, the depth of light penetration is approximately 200 meters. The pelagic, or open ocean, zone constitutes an area that is neither close to the top nor the bottom

MiR34a inhibits Aβ fibril uptake and autophagy flux Regarding the compromised capability of Aβ clearance in AD, we suspected that miR34a may be an indispensable player. To validate this, Aβ42 fibrils were prepared from Aβ42 monomer ex vivo and used to treat glioma A172 and U251 cells. Aβ uptake was blocked by both transient transfection of a miR34a mimic and stable miR34a overexpression (Supplementary Fig. 1B, Fig. 1A), but enhanced by anti-miR34a (Fig. 1B), indicating that miR34a suppressed Aβ

for one hour after. A blood sample was taken from each subject for baseline comparison then at months 6,7, and 12. Patients were coded and, and the sample taken was to measure specific serum IgE level. The following tests were used ELISA assay, luciferase reporter assay, flow cytometry, western blotting, and PCR. Table 1 displayed basic data on demographics such as gender, age, asthma history etc. Figure 1 shows a chart breaking down screening and randomization process of how many patients

Main problem: Chronic Hepatitis C Hepatitis is a systemic disease primarily involving the liver, the term is used to construe inflammation of the liver. One of the main forms of Hepatitis is Hepatitis C; an RNA virus and uses liver cells to create copies of itself, killing those cells in the process. Prior to the identification of the infection in 1989 it was labelled “non-A, non-B” hepatitis (1). HCV blood-borne virus that predominantly infects the cells of the liver and advances to liver inflammation

Introduction Transformation is the process of allowing a cell to be able to accept a gene into itself and allow the gene to become part of the cell 's whole, thus changing its form and expressing the gene 's function. This process was first discovered by the British bacteriologist Frederick Griffith, who was able to transform Streptococcus pneumoniae into a different strain of Strep. in 1928 (1928). This discovery was pivotal in the way we understand gene expression and its engineering. An example

fused to them covalently. The reconstituted activity thus obtained can be used to select for such interacting pairs (Figure 1). To name a few, PCAs employing proteins like murine Dihydrofolate reductase (mDHFR), TEM-1 Beta lactamase (TEM-1 Bla), luciferase, Beta galactosidase, GFP, g3p of M13 filamentous phage have been used successfully for studying protein- protein interactions in bacterial hosts. TEM -1 Beta lactamase: The molecule of choice The focus of this review is the use of one of the most

Noroviruses (NoV) are important human pathogens and a major public health threat. Noroviruses are non-enveloped, single-stranded, positive sense RNA viruses that cause acute gastroenteritis in humans (1-4). Noroviruses have been referred to as ‘cruise ship viruses’ from well-publicized cruise ship outbreaks although these outbreaks account for less than 1% of all outbreaks worldwide (5). The symptoms and illness associated with Norovirus infections is often referred to as ‘stomach flu’, despite little

The Transformation Occurrence of Plasmid in Bacterial Growth Cherline Riche ________________________________________________________________________ Panther ID: 5471556 Crystal Garcia, Joselyn Pozo, Tory Jarett Biology I Lab 1010--Lab Section U55 Abstract: The objective of this experiment was to observe the transformation occurrence with E.coli and the ampicillin resistance gene.When plasmids like lux or pUC18 are added in E.coli, they are more likely to survive in certain

Microorganisms have developed multiple direct and indirect mechanisms that protect them against the environmental stresses they encounter. One of the most severe and widespread problems facing crop production is the degradation of soil quality due to desiccation and salinity, and almost 40% of the world’s land surface is affected by salinity-related problems (Zahran 1999, Veron, 2006). Water, and its availability, is one of the most vital environmental factors to affect the growth and survival of

Module: Molecular micriobiology and epigenetics Dr. Tina Kyndt 19/04/2015 Dries Godderis Faculteit Bio-Ingenieurswetenschappen UGent – Coupure Links 653 – 9000 Gent Paper epigenetics A comparison of DNA-methylation between infants delivered vaginally and by caesarian section 1 Introduction This paper, written for the course ‘Molecular microbiology and epigenetics’ is a summary of two existing articles: ‘Epigenetic modulation at birth - altered DNA-methylation in white blood cells after Caesarean