DNA Technology - gel electrophoresis lab Biol 100L worksheet-1

Worksheet-DNA 2D, synthesis and 3D With this non-graded worksheet, you will put in practice the knowledge you acquired on DNA structures and synthesis. We will go over the answers together in class and you will have plenty of opportunities to ask for clarifications The worksheet should take 60 to 90 minutes to complete and is based around: O Figure labeling O Fill in the blank questions O Multiple-choice questions OMP O HO-P-O- H₂N N 2 N N 3 1 6 Ο 5 OH 4 7 3' carbon 5' carbon Nucleoside Glycosidic bond Phosphate group NH₂ NH₂ H H₂C N N H- 1 2 3 H H O H N Purine Pyrimidine Nucleotide Nucleobase Nucleobase 1 Nucleobase 2 Nucleobase 3 Nucleobase 4 NH 4 NH₂

Instructions: Read 13-2 Manipulating DNA pages 322-323. As you read each section, examine the figures and captions (explanations). Identify any questions you may have. 1) Develop an analogy for the processes researchers use to make changes to DNA. In yo analogy, explain how it is similar to the techniques used in genetic engineering. You can draw a graphic organizer, make a table, or write a few sentences describing your analogy. 2) Devise flowchart that shows the steps to prepare DNA for gel electrophoresis, as well the protocol for setting up and running a gel. You can add diagrams to the flowchart an add detailed notes if you like. English (inited Sate) O Focs ere to search 4 CO RU G\ L B. 2N A\ Alt Ciri

Worksheet-DNA 2D, synthesis and 3D With this non-graded worksheet, you will put in practice the knowledge you acquired on DNA structures and synthesis. We will go over the answers together in class and you will have plenty of opportunities to ask for clarifications The worksheet should take 60 to 90 minutes to complete and is based around: O Figure labeling O Fill in the blank questions O Multiple-choice questions H₂N N 2 N OMPIO HO-P-O- 1 6 Ο 5 OH N 4 3 7 3' carbon 5' carbon Nucleoside Glycosidic bond Phosphate group NH₂ NH₂ H. H₂C N N H 1 2 3 N H H Ο H Purine Pyrimidine Nucleobase 1 Nucleobase 2 Nucleotide Nucleobase Nucleobase 3 Nucleobase 4 4 NH NH₂

helping tags: biochemistry, agarose gel electrophoresis, genomic DNA isolation, gel electrophoresis Please explain your answers thoroughly. Thanks will upvote.

Please solve - no AI answers please. Please refers to image for all detail and explain answers please

helping tags: biochemistry, agarose gel electrophoresis, genomic DNA isolation, gel electrophoresis Please explain your answer thoroughly. Thanks will upvote.

Number 2, please.

Resriction Enzyme Worksheet #1

Primer Designing:

DNA EXTRACTION Materials: knife                                                               1 cup of fruit                              Table salt                                                       clean piece of cloth or strainer for filtering resealable bag                                              dishwashing liquid 70% rubbing alcohol (chilled)                   shot glass (or any transparent and narrow container resembling a test tube)                                                                                                                           How to do the extraction: Before you begin, make sure you have chilled your alcohol in the freezer for at least 3 hours. Room temperature alcohol will not work nearly as well as cold alcohol. Place about 50 mL of alcohol in the freezer to chill it and take it out only when you’re going to need it. (The alcohol will not actually freeze.)   1. The first thing you will need is a sample. Since DNA is found in all living…

DNa Mapping using Restriction enzymes lab: We will be aliquoting and delivering 5 μl of enzyme to each of the experimental tubes. What would happen if you underloaded the enzyme? i.e. you only delivered 3 or 4 μl ? What would see in your gel results?

Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction  Questions: 1. What are the materials used for the polymerase chain reaction?  2. Draw a schematic diagram of the procedure in PCR.  3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride?  6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?

what is the |usage of DNA separation in gel electrophoresis please write the resources under the answer

Can you explain thie each of the statement given i dont really understand the dna recombinant is used as a molecular cloning and application for recombinant dna

וןווד ← Q Lab Report 6 worksheets 314 F22 .DOCX File Edit View Insert Format Tools Help A 100% ¿ Summary Grades for Arysta Visser: 23 x M Uh-oh! There's a problem w X b The restriction EcoRI cleaves X + Untitled spreadsheet - Goog X https://docs.google.com/document/d/1mKY1HIgMPRh1kRDCmX7msBF2yf07-ogT/edit Outline Headings you add to the document will appear here. Normal text Times ... 12 + B I U 2 18. The restriction EcoRI cleaves double-stranded DNA at the sequence 5'-GAATTC-3', the restriction enzyme HindIII cleaves at the sequence 5'-AAGCTT-3', and the restriction enzyme BamHI cleaves at 5'GGATCC-3. An 805 bp circular plasmid is digested with each enzyme individually and then in combination, and the resulting fragment sizes are determined by means of electrophoresis. The results are as follows: 1 Restriction Enzyme(s) EcoRI BamHI HindIII EcoRI and BamHI EcoRI and HindIII BamHI and HindIII 3 Practice ====•=•€ EX Fragment lengths (base pairs) 430 bp, 375 bp 470 bp, 335 bp Lab Report 6…

You need to complete the following steps for an experiment. Explain whether an acrylamide gel or an agarose gel is more appropriate to use for the following experiments. Purifying tRNA from total RNA Isolating a vector insert for cloning Analyzing PCR products

Transcribed Image Text:Complete the following tasks. You discovered that a species of bacteria can break down StyrofoamT (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (GDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: c. Adding ethanol before recovering the DNA extract С. Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus Answer: а. b. Deoxynucleotide triphosphates (DNTPS) Answer: С. Forward and…

please solve this step by step with clear explaination

helping tags: biochemistry, PCR, polymerase chain reaction, PCR cocktail preparation Will upvote, just please help me complete the table and show solutions. Thanks.

Solve 5.

The algorithm or tool in MEGA that is used for multiple sequence alignment of nucleotide/DNA sequences. ClustalX T-coffee MUSCLE Clustal W

#3 question help

it's urgent please solve asap

Question attached as picture. Please answer as soon as you can if possible, thank you!

Sample Gel Electrophoresis: A brother and sister's DNA are cut with the same restriction enzyme and then the resulting DNA fragments are run on a gel next to each other. DRAW THE BANDS on the gel where they would appear: Carissa's DNA was cut into 4 pieces: The pieces were 20 base pairs long, 10bp long, 8 and 5. Christopher's DNA was cut into 5 pieces: They were 25 bp long, 10, 8 and the last two pieces were both 2 bp long. You can make a scole to helo you> 13. What did you do about the 2 DNA fragments that were both 5 base pairs long? 14. How many DNA fragment sizes do these kids have in common? Carissa M Christopher Smith

Please don't provide handwriting solution

DNA extraction, PCR, gel electrophoresis, and DNA sequencing of PCR products. Give a description of these methods and the materials required to perform each of these molecular techniques