Module #5 Formal Post-Lab Report

ica O CHEM-3A-81091-2... ead W ical Part A PCl5 (g) PC13 (1) + Cl2(g) Use A for [PC15], B for [PC13], C for [Cl2]. ΜΕ ΑΣΦ Keq AC1.8 Submit Previous Answers Request Answer × Incorrect; Try Again; 3 attempts remaining Part B 2KNO3(s) = 2KNO2 (s) + O2(g) Use A for [KNO3], B for [KNO2], C for [02]. Keq = ΜΕ ΑΣΦ Submit Part C Request Answer Pollen tomorrow ? ?

An exploration of any one application of Spectroscopy (Absorption or Emission), Mass Spectrometry (MS) - (ANY kind of mass spec)  Pick one location/project/bit of research where the data is used and explore.  Discuss on what is measured (what instrument) and why and how.  You don't have to fully teach the instrument but spend a paragraph giving the very basics of what is measured and how.  And discuss the application you discovered - just find a place the measurement is used and explain what they do - what they measure and when it tells them.

PLEASE ANSWER ASAP

Carbon monoxide is another common poison found in the forensic laboratory. Explain how UV-visible spectrophotometry is applied to quantify carbon monoxide in blood samples.

4. When plotting a standard curve in absorption spectrophotometry, one uses Abs values instead of %T values. Explain why you need to use Abs values instead of %T values when constructing a standard curve (or a linear Beer's law Plot)

Given C6H12O2 chemical formula. chemical shift , intergration, multiplicity and interpretation needed.

Assuming there are three special isotopes mixture of cadmium, Cd110, Cd112 and Cd114. If He-Cd laser is operating at temperature 25°C at wavelength of 450 nm. Find the Doppler broadening for these three isotopes.

Find information on fluorescent dye Fluo-3: Display its structure Briefly describe its purpose and the mechanism of operation Display its fluorescence spectra Display transmission spectra of commercially available optical filters required for flow cytometry measurements of cells stained with Fluo-3 (see lecture notes on where to look)

Match the wavelength range of electromagnetic radiation to the type of spectroscopy. > 4000-600 cm-1 10 - 380 nm 380-780 nm 1. IR Spectroscopy 2. Ultra Violet Spectroscopy 3. Visible Spectroscopy

26. What exactly is flame atomic absorbtion spectrometer?

Explain the principles of UV-Vis Spectrometer

Please don't provide handwriting solution

True or False 1. Photodiode arrays are devices within the instrument that help amplify the signal of a sample. 2. Atomic spectroscopy requires the atomization of a sample, which involves nebulization and eventually volatilization to form an elemental vapor. 3. Organic compounds are normally analyzed in atomic spectroscopy despite their small electronic transition range.

The data given below was generated by a student in Bio 240L during a Bradford Assay. A standard curve was generated by preparing a set of standard samples of protein (bovine serum albumin) and adding the dye Coomassie Brilliant Blue, When the protein binds to Coomassie Brilliant Blue it absorbed light at 595 nm with an extinction coefficient of 43,000 M-1 cm-1. After the dye binding reaction was complete, absorbance values for each protein standard solution were measured at 595 nm and recorded. Standard # 1 2 13 4 Concentration of protein binded to Coomassie Brilliant Blue (M) 6.35x10-7 1.23x10-6 3.85x10-6 5.68x10-6 OOO Absorbance at 595 nm 0.093 0.176 0.560 0.813 Prepare a standard curve with the student generated data. What is the slope from the standard curve rounded to four sig figs and no units? 1.4 143398 6.972x10-6 1.434x105

Chemistry Instrumental Analysis Class The experiment is OES: Cleaning Validation of removal of pharmaceutical drugs and calibration standards by fluoresence spectroscopy - Why the instrument use two detectors and monochromators? What type of monochromators are used in our instrument? - Do you think that a similar experiment can be prepared for the analysis of naproxen and benzoic acid? Explain.

Spectral Analysis IR Analysis Interpret the IR spectrum. Transmitance 0.9 0.8 0.7 0.6 0.5 0.4 0.3 0.2 3500 3000 Infrared Spectrum 2500 Wavenumbers (cm-1) 2000 Cha 1500 1000

Sketch the main components of an FTIR infrared absorption spectrophotometer.

Assume that the first line of the rotational spectrum (J = 0 → J = 1) of CO is 3.8424 cm-1. Calculate the length of the CO bond in picometer (10-12 m). To mark the answer, neglect the fractional part and do not write the unit. Information: mass of carbon: 12 c.u. mass of oxygen: 16 c.u. h = 6,6261 x 10-34 J.s   π = 3,1416 c = 2,9979 x108 m.s-1 N = 6,0221 x 1023 mol-1

How does pipetting laboratory techniques affect absorbance values when using UV-Vis Spectrophotometry?

Formula: C4H100 Spectroscopy Reference 100 80- 60 40 20- 4000 3500 3000 2500 2000 1500 1000 6H 1H 1H 2H 4.0 3.5 3.0 2.5 2.0 1.5 1.0 PPM 0.5 1248 C TLT 6EE

5. Determine the percent transmittance of B-carotene, C40H56 that has an absorbance of 0.5 when analyse using a UV-Visible spectrophotometer. A. 6.83% B. 31.6% C. 0.32 % D. 68.3% 6. The equation of Beer Lambert's Law is A = abc. If the 'b' is distance, 'a' is absorptivity, and 'A' is absorbance, what does the 'c' represent? A. Intensity B. Analyte concentration C. Analyte transmittance D. Frequency 7. All the following is an example of photon detectors EXCEPT: A. Vacuum phototube B. Silicon photodiodes C. Bolometers D. Photomultiplier tubes 8. C=O bond stretch occurs at 1750 cm1 and C-O stretch occurs at 1100 cm1 because: A. The C-O has a single bond which is a stronger bond, so it vibrates at a higher frequency than the C=O. B. The C-O has a single bond which is a stronger bond, so it vibrates at a lower frequency than the C=O. C. The C=O has a double bond which is a stronger bond, so it vibrates at a lower frequency than the C-O. D. The C=O has a double bond which is a stronger bond,…

Chemistry Read the Hydrogen nuclear magnetic resonance spectrum ,considering that it is from the biseugenol molecule , write as much information as this graph can give.

Explain your answer.

1FbhGb6cd_1-5c/IV_Ivqu J4002epq/z4kbhpYZSIA opy ✩✩ IS Extensions Help I text Calibri. 12 +BIU A d f5 A % 5 T f6 1 5 J 1. Why is it recommended to have a carbon monoxide detector on each floor of a home? Page 4: Water Pollution: Questions i M 6 2. What impact would the increased use of electric vehicles have on environmental damage caused by acid rain? Page 3: More Air Pollution Issues: Questions 4. The COVID-19 pandemic forced many people to work from home for extended periods. During these periods, many cities reported unusually low levels of smog. Suggest an explanation for this observation. 5. Explain how ozone can be both harmful and beneficial. 3. Why is ground level ozone most likely to reach its highest concentrations on hot, sunny days during periods of heavy traffic in large cities? f7 Y []») 3 6. India relies heavily on burning coal to generate electricity. How does India's reliance on coal contribute to the poor air quality experienced in many of India's major cities? & 7 18…

Name of Experiment: Fluorescence Spectroscopy Q1: Purpose of experiment Q2: Introduction

Answer the question concisely. Maximum of 5 sentences.   Differentiate spectroscopy and spectrophotometry. Cite an example and discuss it in a chemical perspective.

A calibration curve is made for the absorbance as a function of concentration of blue dye (in mg/L).  The slope of the curve is 0.131; the y-intercept is -0.01869; the correlation is 0.993.  If a consumer sample has an absorbance of 0.120 on the same instrument, what is its concentration of blue dye, in mg/L? a. 0.034 b. 0.935 c. 0.993 d. 1.06

Instrumentation of IR spectroscopy?  Please answer at your own words.

How does the Hollow Cathode Lamp work during the quantitative analysis of Iron (Fe) in Atomic Absorption Spectroscopy?