2024_CHEN5379_HW1a_Group7

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Apr 3, 2024

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Lamar University CHEN 5379 – APPL BIOPROCESS FERMENTATION Homework -1 Instructor: Dr. Clayton Jeffreys Group 7:- Sri harsha vakkantula (L20580616) Nikhil lekkala (L205874412 )
Problem Statement: We will consider the bioreaction for the conversion of glucose, ammonia and oxygen into biomass, CO2 and water 𝐶 6 𝐻 12 𝑂 6 + 𝑁𝐻 3 + 𝑂 2 𝑏𝑖𝑜𝑚𝑎𝑠𝑠 + 𝐶𝑂 2 + 𝐻 2 0 Based on your parameters, set up a continuous SuperPro Designer simulation to produce as much dry (>99%) biomass as possible. Include your work (calculations, process flow diagram, SuperPro Designer file). source:- saccharomyces cerevisiae formula:- CH 1.64 O 0.52 N 0.16 calculations:- C 6 H 12 O 6 +aO 2 +bNH 3 C.CH 1 . 64 O 0.52 N 0.16 +dCO 2 +eh 2 0 C-balance :- 6=c+d (1) O-balance:- 6+2a=0.52c+2d+e (2) N- balance:- b=0.16 (3) H-balance:-12+3b=1.64c+2e (4) From the equation:- A=1.25,b=0.7378,c=4.612,d=1.3885,e=3.325
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Stoichiometry balanced reaction C 6 H 2 O 6 +1.25 0 2 +0.7378 NH 3 4.612 CH 1.64 O 0.52 N 0.16 +1.3885 CO 2 +3.325 H 2 O Task 1 -Stoichiometric Fermentation of Glucose to biomass production. 1) 1.1) process flow diagram
Figure – 1. Flowchart of biomass production by Glucose. The flowchart illustrates the stoichiometric reaction of biomass production from glucose using ammonia as the nitrogen source, adding the necessary pure components of reactants and products, and calculating the stoichiometric data of those components with a 99% conversion rate as the final products. 1.2) stream glucose Figure 2:-feed subtract
1.3) stoichiometric material balance Figure 3:-For mass Figure 4:-For molar 1.4)Kinetics data for fermentation:-
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Figure 5 1.5) recycle stream Figure 6:-recycle stream 1.6) centrifugation
1.7) rotary vacuum filtration Figure 9:-rotary vacuum filtration 1.8) Product stream
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Figure 10:-output result 89% glucose to biomass 1.9) vent emission data:- Figure 11
Conclusion:- 1 ) With the help of kinetic fermenter and using kinetic data such as Ks = 1200 mg/L, mumax = 0.5/hr, alpha = 1. All yield coefficients are YX/S = 0.62 Kg biomass/Kg glucose. 2) Using feed streams to add pure components and air mixtures, which react in a kinetic fermenter to produce biomass, and other simulation data to describe the process, creating a flow chart that illustrates how biomass is produced from the substrate, glucose.