1. What is Du? ESTIONS ON LABORATORY ASSAY NO.7 2. Why do we need test for Du when weak or no reaction is obtained in Rh typing? 3. What is required to demonstrate the presence of cells carrying a weak D antigen? 4. Explain the mechanism behind the existence Du. 5. Cite possible situations in which an attending physician would request for an emergency screening for the presence of "D" antigen.
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- What is the working principle of Lateral flowimmunochromatographic assays (LFIAs)? Supported with a figure and reference plz1. Give the advantages of using tube method over the slide method. Why do you have to incubate the tube method in Rh typing? 2. Give the clinical importance of determining the Rh status of a patient? 3. Define HDN and explain the effect of Rh(D) antigen. What is the preventive measure of preventing D-negative mothers from forming anti-D? 4. What are the symptoms in Rh-mediated hemolytic transfusion reactions?Give typed explanation of both question 1) What is Linkage disequilibrium as applied to HLA antigens? 2) What is the use of HLA molecules in determining paternity testing, disease association, platelet transfusion, TRALI, and transplants?
- 1. What is the purpose of Rh view bow? What is the optimum temperature for detection of Rh antigen? 2. Give the different types of Rh antigen typing reagents accdg. to the sources. Explain each.state the significance of nontreponemal antiliid (reagin) antibodies in serological testing.RAPID PLASMA REAGIN (RPR) TEST RPR is use in the non-treponemal flocculation test for the qualitative and semiquantitative determination of regain antibodies in serum or plasma Materials: RPR Antigen: Suspension of carbon approximately 0.2 g/L, 0.003% Cardiolipin, 0.02% Lecithin and 0.09% Cholesterol. Positive Control: Serum containing antibodies against Treponema pallidum. Negative Control: Serum free of antibodies against Treponema pallidum. Stirrers Disposable test slides Micropipettes capable of dispensing 50 ul and 16 ul Disposable serum pipets Test tubes Test cards Rotator (set at 100 rpm) Isotonic saline: 0.9% NaCl Principle: RPR is a modified form of VDRL Antigen which contains carbon particles to improve the visual reading of the result. When binding occurs between cholesterol / cardiolipin / lecithin in the reagent and the reagin antibodies in the sample, the results can be seen macroscopically in…
- RAPID PLASMA REAGIN (RPR) TEST RPR is use in the non-treponemal flocculation test for the qualitative and semiquantitative determination of regain antibodies in serum or plasma Materials: RPR Antigen: Suspension of carbon approximately 0.2 g/L, 0.003% Cardiolipin, 0.02% Lecithin and 0.09% Cholesterol. Positive Control: Serum containing antibodies against Treponema pallidum. Negative Control: Serum free of antibodies against Treponema pallidum. Stirrers Disposable test slides Micropipettes capable of dispensing 50 ul and 16 ul Disposable serum pipets Test tubes Test cards Rotator (set at 100 rpm) Isotonic saline: 0.9% NaCl Principle: RPR is a modified form of VDRL Antigen which contains carbon particles to improve the visual reading of the result. When binding occurs between cholesterol / cardiolipin / lecithin in the reagent and the reagin antibodies in the sample, the results can be seen macroscopically in…a) Why is testing for rhesus (Rh) antigens and antibodies different from ABO testing?1.In radioimmunoassay the following reagents (parameters) are constant except A. Antibody (antiserum) B. Radiolabeled hormone (tracer) or (hot) C. Unlabeled hormone (cold) D. A and C E. None of the above
- Explain the principles of the different methods used for the diagnosis of HDFN. Define Rh-immune globulin and describe its function. Identify the indications and contraindications for administration of Rh-immune globulin. List the tests used for detecting fetomaternal hemorrhage. Outline the protocol for testing maternal and cord blood in cases of suspected HDFN.1. How will you differentiate VDRL from RPR Card test? 2. Give the composition of VDRL reagent and their function.1) Discuss the formation of ABH antigens 2) Enumerate the components of CPDA-1 and discuss their respective functions