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- A TLC plate showed two spots with Rf values of 0.25 and 0.26. The plate was removed from the developing chamber, the residual solvent was allowed to evaporate from the plate, and then the plate was returned to the developing chamber. What would you expect to see after the second development was complete?5. Would you expect that changing the solvent will change the Rf value of the specific solute? Why?6. Suggest simple reasons why the Ni2+ and Fe3+ ions have different Rf values?7. What forces causes the eluting solution to move the chromatographic support material?Describe and explain the effect(s) on your result for the following possible experimental errors. In each case, specify the component(s) whose percentage(s) would be too high or too low and explain your rationale why these would be changed. (a) After adding DCM to the counterfeit pharmaceutical at the beginning, you didn’t stir or shake the mixture before filtering. (b) Washing the sucrose recovered during the first vacuum filtration with water rather than DCM. (c) During the NaHCO3 extraction, you failed to mix the organic and aqueous layers thoroughly. - B (d) You mistakenly extracted the DCM solution with 5% HCl rather than 5% NaHCO3. (e) You neutralised the NaHCO3 solution to pH7 rather than pH2.
- Using thin-layer chromatography showed that the black dye used in a brand of writing ink contained blue, red, orange and yellow components. The Rf values of these substances using ethanol as the solvent are 0.63, 0.37, 0.79 and 0.16, respectively. How far apart would the blue and yellow components be after the solvent front had moved 7.5 cm from the origin?a) How does mass spectrometry identify organic compounds? b) Describe the use and function of Quadrupole Analyzers ? c) One of the ionization sources for atomic mass spectrometry (MS) is Inductively Coupled Plasma (ICP). Explain the need for an interface in the ICPMS instrument and how can it be achieved.Imagine that the blue component of a given mixture moved 2.5 cm from the oigin or starting line, and the solvent had traveled 10.0cm, What is the Rf for the blue dye A .4.4 B 0.25 C. 7.5 D. 25
- Estimate the elution-order of the three compounds shown below in a TLC-analysis. which compound elutes fastest, which elute slowest, motivate your answer.To what chemical class do each compound belong to?The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.671. The heat flows measured in this experiment were actually not for the simple neutralization of a proton and hydroxide ion (as indicated in the Introduction for Choice Ill); rather they include contributions based on the fact that these species are hydrated in aqueous solution. What does it mean to say that a proton is hydrated, and how will the heat of hydration affect the measured heat flow for a neutralization reaction?
- 1. In the chromatography plate there are three solutes that move forward: 2cm, 2.5cm and 3cm Which will be more polar? 2. What information does the infrared of the spectroscospy offer?What is the Rf value for a compound if it moves 3.0 cm from the origin when the initial spot is developed and the solvent front is 12.0 cm from the origin?A student places an unknown sample on a thin-layer chromatography plate and develops it in dichloromethane as a solvent. Only one spot whose Rf is 0.98 is observed. Does this result indicate that the unknown material is a pure compound? If not a pure compound, suggest an alternate procedure to verify the purity of the sample.