13- Which statement is not correct for primer design criteria? a) In the length of 18-22 nt b) 60-80% GC content Forward and reverse should have similar Tm d) The repeats of di-nucleotide formations should be avoided Boş bırak

Q: Reagents necessary for DNA sequencing by chain termination include: (MARK ALL THAT APPLY) Group of…

A: Change termination sequencing is also known as Sanger sequencing that is used for the identification…

Q: 13- Which of the following substitutions do not produce hidden mutations, that can not be observed…

A: The correct answer is (d)single substitution.

Q: 9. Recombinant DNA could be transformed into a cell through various methods such as: Transformation…

A: Process of taking up nacked DNA inside the cells is called as transformation. The methods involving…

Q: 13. What does the Master Mix contain? 14. What are primers? Why is a primer added? 15. Once the…

A: Bacterial indemnification in the laboratory uses following traits during the procedure:  Gram…

Q: Listed below are three strong antiviral drugs that inhibit aspects of DNA replication. Given their…

A: The antivirus drugs are used to disable the ability of flu viruses. Some of the given antivirus…

Q: 12. A sequence of nucleotides is shown below, along with an indication of an origin of replication:…

A: Replication is the process of synthesizing new DNA from the parental DNA.

Q: Discuss the reasons proteins were generally favored over DNA as the genetic material before 1940.…

A: DNA and RNA are considered as a genetic material. DNA is inherited or transferred from parents to…

Q: The A-T base pair has more hydrogen bonding than the C-G base pair. True  False

A: Adnene, thymine, cytosine and guanine are the nitrogenous bases which are part of nucleotides…

Q: 1. Explain why the primary structure sequence -Lys-Leu-Trp-Asp- may promote a-helix formation while…

A: The formation of α helix is occurred due to attachment of H of N of Cα of first (n) amino acid to…

Q: 1. Under each sequence of bases, write the sequence of bases complementary to each section of DNA in…

A: Introduction :  The primary nucleobases are cytosine (DNA and RNA), guanine (DNA and RNA), adenine…

Q: DNA polymerase uses which of the following catalytic strategies: Group of answer choices Metal ion…

A: The correct option is Metal ion catalysis. Explanation: During the extension of a DNA strand…

Q: 5.If the following two primers 3'AAGS' S'CCG3' were used to facilitate the polymerase chain reaction…

A: Deoxyribonucleic acid (DNA) refers to the polymer that is made of nucleotides. A nucleotide…

Q: 2. Provide the sequences of the template and coding strands of a DNA double helix that was used to…

A: The RNA or ribonucleic acid is produced from the DNA by the transcription process. RNA is used for…

Q: 5. Show the separation pattern of the following DNA molecules on an agarose gel electrophoresis. 5…

A: Forms of DNA gives different band pattern.

Q: 3. For this short DNA segment, a. identify the 5' end and the 3' end of the molecule. b. circle the…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Why are the following reagents used? Neutralizing solution (Plasmid isolation)    Isopropanol…

A: 1. DNA Neutralizing Solution can be utilised in separating of plasmid DNA by alkaline lysis…

Q: 6. Which of the following DNA sample composition is expected to have the highest boiling point? A.…

A: As per Chargaff's rule, number of guanine is equal to number of cytosine and amount of thymine is…

Q: Genosensors are biosensors based on the principle of complementarity and hybridization of DNA…

A: Biosensors and genosensor continue to offer solutions and control of various processes across a…

Q: 25. Which sequence includes RNA that's complementary to the DNA sequence 5'-TATTCGC-3'? (Hint: in…

A: Adenine(A) and guanine(G) are purines ; cytosine(C) and thymine(T)/uracil(U) are pyrimidines , both…

Q: most STR fragments used in human forensic analysis are comprised of_____ repeats

A: STR Stands for short tandem repeats, these are short sequences in DNA that make large subunits…

Q: 28. What type of mutation is seen here? WT: 5′-AUG GCU AGA GUU GAA AAA-3′…

A:

Q: 8. For each of the following DNA template strands a. 3' TACGGC 5' b. 3' CCATTA 5' Determine: a. the…

A: The heterogenous nuclear ribonucleoprotein (hnRNP) is the  initial step of synthesizing mRNA during…

Q: What is the chemic name and formula of the precipitate for the test for phosphate? 2.suppose biuret…

A: Dear students, As the given question has multiple questions and here it is not specified which…

Q: I need question 38

A: in the phase of oxidative stress, damage to the DNA is quite common. the ROS can attach to certain…

Q: 12. If this is the base sequence of DNA, what is the resulting AA sequence for the following…

A: Mutations When the sequence of DNA is altered it is said that the DNA is mutated. Mutations causes…

Q: 1. Where is the cleavable blocker located on the modified nucleotides used in Illumina sequencing?

A: Illumina sequencing is developed by Solexa and was subsequently acquired by Illumina.  This…

Q: 3. Given the following short DNA duplex of sequence (5' → 3'),…

A: A nucleotide is a fundamental DNA building block and structural unit. It has a nitrogenous base, a…

Q: 7. Describe the mutation that causes sickle cell anemia. a. What is the change that occurs in DNA?…

A:

Q: 21. Which lane shows a digest with Bamtll only? a. I b.I c. II d. IV e. V 22. Which lane shows a…

A: Question - 21. Which lane shows a digest with Bambil only?  a.l     b. ll     c. III     d. IV…

Q: 10. Analyse the following DNA sequences and identify and explain the anomalies you observe. If there…

A: A nucleotide is the most fundamental component of nucleic acids (RNA and DNA). A sugar molecule…

Q: 7. Superhelical Density Bacteriophage A infects E. coli by integrating its DNA into the bacterial…

A: Superhelical density is a quantitative measure of the supercoiling degree of the DNA molecule. It is…

Q: 8.Bacteria X contains the double stranded DNA sequence below. Our task is to design a labelled…

A: Primer - A primer, as related to genomics, is a short single-stranded DNA fragment that anneals with…

Q: 1.(a) what are the possible secondary structures of the primer and what are there explain with…

A: There are two types of secondary structures that are considered for primers. The structure and the…

Q: (3) Hfr strains chosen for use in conjugation studies have the locus that controls antibiotic or…

A: Introduction :- There are three main methods of horizontal gene transfer in bacteria :- conjugation…

Q: 6. H₂C HO Which of the following dinucleotides can be methylated by DNA methyltransferase? NH₂ NH₂ 9…

A: Since you have asked multiple questions, we will answer only first question for you. In order to get…

Q: 47 If base #6 is changed from A to T, what type of mutation is this?? ANTISENSE STRAND 5' ATTTGACGG…

A: Antisense strand is also known as template strand. This strand is used to produce mRNA during…

Q: 11. Consider the sequence shown, determine the complementary RNA and the amino acids DNA TAC GTA TTT…

A: In this question, we are given sequence of DNA from which we have to determine RNA and protein…

Q: 2. In the given segment in problem 1, illustrate and indicate the direction of synthesis of: a.…

A: The replication process begins with the unwinding of the polynucleotide strand, which forms a…

Q: 22. Single base substitution is   A. Point mutation   B. Deletion   C. Substitution   D.…

A: Mutations These are defined as the alterations in the sequence of the DNA due to exposure of…

Q: Does 5-bromouracil cause a transition or a transversion?

A: The transition mutation refers to a point mutation that replaces purine with another purine or…

Q: 1) What is the purpose of hydrolyzing the RNA before conducting biochemical tests?

A: RNA or Ribonucleic acid is a complex compound that is polymeric and helps in the coding, decoding…

Q: 16. Why is re-circularized insert generally not a problem in cloning procedures, while re-…

A: Cloning or DNA cloning is the process of making multiple identical copies of the gene of interest by…

Q: 6. Which of the following DNA sample composition is expected to have the highest boiling point? A.…

A: Since you have asked multiple questions we will answer the first question for you. If you want any…

Q: During nucleic acid hybridization, the probe is labelled    for DNA stability   to increase…

A: As per our honor code, we are allowed to answer one question at a time. You have posted multiple…

Q: 72) In case of RNA dependent RNA polymerase in Newcastle disease virus (NDV), is a a. Its template…

A: Introduction - Newcastle disease virus (NDV) is a virus that causes serious infection in birds and…

Q: 3b) In the real world, where "wobble" pairing is possible, what is the minimum number of tRNAs…

A: The process of formation of mRNA(messenger ribonucleic acid) molecules on the DNA(deoxyribonucleic…

Q: 6. In 1928, before DNA was recognized as the hereditary material, F Griffith performed a series of…

A: A is Heat killed S-strain which are non virulent as it does not killed the mice and is undetected. B…

Violence

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