[3] Instructions: Read each excerpt from a research paper and identify the dependent, independent, controlled variable (at least 1), experimental group/treatment, and control group. Antibacterial Assay The test solution of each extract was prepared by dissolving 0.1g of the plant extract separately. 1.0cm3 of dimethyl sulphoxide (DMSO) to get a concentration of 100mg/cm3. The antibacterial activity was performed by filter paper disc diffusion technique. Filter paper disc (Whatman No 1.6 mm diameter) were placed in glass Petri dish and sterilized in a hot air oven. Iwu et al 2018b, the media (10g nutrient Agar in 200cm3 distilled water, autoclaved at 115°C for 30 minutes) was cooled to 50°C. The sterile nutrient Agar media were poured into the sterile Petri dish and allowed to solidify. The bacteria were swabbed with a sterile wire loop. Each disc was impregnated with 0.2cm3 of plant extract. Standard antibiotic Ciprofloxacin was used as a control on a disc with DMSO 100 mg/cm3. The discs were used after drying them in an incubator 40°C to remove any trace of solvent. Discs were introduced into the surface of the medium. The plates were microbated at 37°C for 24 hours to obtain zones of inhibition. The experiments were repeated three times for each extract and twice for reference antibiotics to minimize error and the average of these values was recorded.” Independent variable: Dependent variable: Controlled variable: Experimental Group: Control group:
[3] Instructions: Read each excerpt from a research paper and identify the dependent, independent, controlled variable (at least 1), experimental group/treatment, and control group.
- Antibacterial Assay
The test solution of each extract was prepared by dissolving 0.1g of the plant extract separately. 1.0cm3 of dimethyl sulphoxide (DMSO) to get a concentration of 100mg/cm3. The antibacterial activity was performed by filter paper disc diffusion technique. Filter paper disc (Whatman No 1.6 mm diameter) were placed in glass Petri dish and sterilized in a hot air oven. Iwu et al 2018b, the media (10g nutrient Agar in 200cm3 distilled water, autoclaved at 115°C for 30 minutes) was cooled to 50°C. The sterile nutrient Agar media were poured into the sterile Petri dish and allowed to solidify. The bacteria were swabbed with a sterile wire loop. Each disc was impregnated with 0.2cm3 of plant extract. Standard antibiotic Ciprofloxacin was used as a control on a disc with DMSO 100 mg/cm3. The discs were used after drying them in an incubator 40°C to remove any trace of solvent. Discs were introduced into the surface of the medium. The plates were microbated at 37°C for 24 hours to obtain zones of inhibition. The experiments were repeated three times for each extract and twice for reference antibiotics to minimize error and the average of these values was recorded.”
| Independent variable: | |
| Dependent variable: | |
| Controlled variable: | |
| Experimental Group: | |
| Control group: |
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