4. Which ranges of microbial colonycount is ideal standard for a good1,plate in Pour Plate technique? Checkall that apply. *O TNTC (too numerous to count)O 25-250 coloniesO 10-1000 coloniesO 30-300 colonies5. How many colony-forming units(CFUS) of bacteria are there in a gramof soil if 120 colonies grow in anutrient agar (NA) plate with 1 mlinoculum from 1:10,000 dilution? *120O 1,200120.0001,200,0006. How much volume of microbialinoculum will be dispensed into asterile nutrient agar plate in SpreadPlate technique of bacterial isolation?0.1 ml1 ml10 mlO 100 ml7. Which materials are to be preparedwhen using Spread Plate techniquefor bacterial isolation from watersample of oligotrophic Lake Lanao?Check all that apply. *O lake water sampleO sterile nutrient agar plateO sterilizable glass rodO flame source

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4. Which ranges of microbial colony 1, count is ideal standard for a good plate in Pour Plate technique? Check all that apply. * O TNTC (too numerous to count) O 25-250 colonies 10-1000 colonies O 30-300 colonies 5. How many colony-forming units (CFUS) of bacteria are there in a gram of soil if 120 colonies grow in a nutrient agar (NA) plate with 1 ml inoculum from 1:10,000 dilution? * O 120 1,200 O 120,000 O 1,200,000 6. How much volume of microbial inoculum will be dispensed into a sterile nutrient agar plate in Spread Plate technique of bacterial isolation? O 0.1 ml O 1 ml O 10 ml O 100 ml 7. Which materials are to be prepared when using Spread Plate technique for bacterial isolation from water sample of oligotrophic Lake Lanao? Check all that apply. * O lake water sample sterile nutrient agar plate O sterilizable glass rod O flame source O O O O

4. Which ranges of microbial colony 1, count is ideal standard for a good plate in Pour Plate technique? Check all that apply. * O TNTC (too numerous to count) O 25-250 colonies 10-1000 colonies O 30-300 colonies 5. How many colony-forming units (CFUS) of bacteria are there in a gram of soil if 120 colonies grow in a nutrient agar (NA) plate with 1 ml inoculum from 1:10,000 dilution? * O 120 1,200 O 120,000 O 1,200,000 6. How much volume of microbial inoculum will be dispensed into a sterile nutrient agar plate in Spread Plate technique of bacterial isolation? O 0.1 ml O 1 ml O 10 ml O 100 ml 7. Which materials are to be prepared when using Spread Plate technique for bacterial isolation from water sample of oligotrophic Lake Lanao? Check all that apply. * O lake water sample sterile nutrient agar plate O sterilizable glass rod O flame source O O O O

Comprehensive Medical Assisting: Administrative and Clinical Competencies (MindTap Course List)

6th Edition

ISBN:9781305964792

Author:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Publisher:Wilburta Q. Lindh, Carol D. Tamparo, Barbara M. Dahl, Julie Morris, Cindy Correa

Chapter42: Basic Microbiology

Section: Chapter Questions

Problem 4CR

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3. One gram of soil was suspended in 9 ml saline and thoroughly mixed. From this suspension, 4 serial, 1:10 dilutions were made. 200 hundred microliters of each of the last four serial dilutions were used for spread plating on TY media, resulting in 5600 colonies, 412 colonies, 54 colonies and 8 colonies. What do you calculate as the bacterial concentration in the soil? (For your information 1 gram = 1 ml)
4. All the following can be autoclaved EXCEPT: a) Biological material b) Glass ware c) Radioactive material d) Broth and gel media for growing bacteria5. The autoclave raises the atmospheric pressure to _____ : a) 10 psi b) 15 psi c) 20 k joules d) 25 watts6. In the experiment with serial dilutions, the plates which should be used for the final count for colonies should have a range of _______ CFU in order to have a reliable estimate: a) <10 b)10-15 c) 15-20 d) none of the above
1. Which materials are to be prepared when using Spread Plate technique for bacterial isolation from water sample of oligotrophic Lake Lanao? Check all that apply. ( ) lake water sample ( ) sterile nutrient agar plate ( ) sterilizable glass rod ( ) flame source 2. Which ranges of microbial colony count is ideal standard for a good plate in Pour Plate technique? Check all that apply. ( ) TNTC (too numerous to count) ( ) 25-250 colonies ( ) 10-1000 colonies ( ) 30-300 colonies
12/ Which of the following factors increases biofilm accumulation? (select all that apply) V A. malocclusion V B. smooth surfaces V C. calculus V D. restorations E. use of fluoridated 9/ During biofilm formation, microcolonies form in layers as the bacterial colonies multiply. With increased size, colonies meet and coalesce to form a complex microbial community. © A Both statements are true. A © B Both statements are false. C. The first statement is true, and the second statement is false. D. The first statement is false, and the second statement is true. Question 11 Which of the following is found in a disease periodontal pocket? © A Gram positive cocci and lactobacillus species. O B. Primarily jam negative motile rods and spirochetes • C. Gram positive aerobes © D. Keystone pathogens.
1. What is the essence of the II stage of isolation of a pure culture of aerobic and anaerobic microorganisms? How is the purity of the selected culture evaluated? What are the characteristics of the growth of microorganisms on liquid nutrient media?
1. You were given a mixed nutrient agar broth culture of bacteria. a. How will you determine the different types of bacteria present in the mixed culture? b. How will you make a pure culture of these bacteria in a slant nutrient agar? c. How will you identify these bacteria?
1. Give examples of specific applications for different types of solid tubed media 2. Give 5 examples of microorganisms that may be grown in Nutrient Broth. 3. Discuss the implication/s if the spore strip was missed out during the preparation of the sample for autoclave and possible remedy for this scenario.
PLEASE ANS ASAP 7. Typhoid fever is caused by a species of: a . Escherichia b. Campylobacter c. Shigella. d. Salmonella 8. At the end of the Gram -staining procedure , Gram -positive bacteria will be: a. blue to purple b . pink to red c. orange. d. green 9. Which one of the following types of culture media is selective and differential? a. Phenylethyl alcohol agar b . Blood agar c. Thayer-Martin agar d. MacConkey agar 10. Which one of the following methods of antimicrobial susceptibility testing combines some of the properties of two other methods and involves adding a plastic strip to the agar? a. Gradient diffusion method b. Agar macrodilution method c. Disk diffusion method d. Broth microdilution method 11. Most ATP molecules are produced during which phase of aerobia respiration? a. Fermentation b. Krebs cycle c. Glycolysis d. Electron transport chain 12. The goal of disinfection is to eliminate ____ whereas the goal of sterile technique is to exclude ____ a.…
5. The autoclave raises the atmospheric pressure to _____ : a) 10 psi b) 15 psi c) 20 k joules d) 25 watts 6. In the experiment with serial dilutions, the plates which should be used for the final count for colonies should have a range of _______ CFU in order to have a reliable estimate: a) <10 b)10-15 c) 15-20 d) none of the above 7. The temperature to incubate the bacteria in the above mentioned experiment should be __: a) 22 oC b) 37 oC c) 98 oC d) 37 o
You prepared 10-7, 10-8, and 10-9 dilutions of a bacterial suspension in sterile saline. Then you plated 0.15mL if each dilution onto each of 3 plates of nutrient agar. After incubation, the colonies were way too numerous to counton the plates prepared from the 10-7 dilution. The plates from the 10-8 dilution had 355, 360, and 350 colonies. The platesfrom the 10-9 dilution had 115, 117, and 118 colonies. Determine the concentration of colony forming units (cfu/mL) inthe original bacterial suspension
1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.
1. List down the factors that can cause contamination to your culture. 2. Why does the pH calibration is done before sterilization but pO2 calibration is done after sterilization? 3. What are the differences between impeller design for microbial, mammalian, and plant bioreactor and why they are designed in different ways?