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Direct repair of pyrimidine dimer formation in E. Coli can be accomplished by
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- Extrachromosomal DNA is critical to the antibiotic resistance found in microorganisms, how do these DNA elements account for this phenomena?In the traditional alkaline lysis method of Solution 1, what is the purpose of RNAse A?What is the function of conjugation reactions in the biotransformation process?
- What would the effect be if there was a substitution of one nucleotide for another?In producing genetically engineered human insulin in bacteria, why is it important to use the samerestriction enzyme to cut both the human DNA and the bacterial plasmid?Why do E. coli cells with a defective lacZ gene fail to show galactoside permease activity after the addition of lactose in the absence of glucose?
- Cytosine can be deaminated to form Uracil What type of mutation is this classified as? Discuss what happens to the base-pairing properties from switching from C to U? When U is replicated in two rounds of synthesis, what substitution does this result in? Before Uracil alters the DNA during replication, what repair system can be used to correct this error? Describe how this type of DNA repair works?Escherichia coli and other bacteria methylate adenines on the original strand to distinguish the original strand from the newly replicated strand of DNA. Why is this distinction important?In DNA double digestion, what does star activity mean and why should it be considered in choosing buffers?
- In the procedure shown, why was it necessary to link thecoding sequence for the A or B chains to the sequence forβ-galactosidase? How were the A or B chains separated fromβ-galactosidase after the fusion protein was synthesized in E. coli?Based on the N-terminal amino acid sequence, what is the approximate half-life of the protein after methionine is cleaved off?Why would a mutation in a somatic cell of a multicellular organism escape detection?