interphase FISH is used on fixed material from a solid tumour, rather than metaphase FISH
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Fluorescence in-situ hybridisation (FISH) analysis can be performed on fixed pathological tumour sections. Briefly outline why interphase FISH is used on fixed material from a solid tumour, rather than metaphase FISH
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- What are the different categories of cell viability assays? Describe one of them briefly. Define the role (aim of use) of Trypan blue dye in cell culture studies.Human Immunodeficiency Virus (HIV) Principle: The assay starts with a sample applied to the sample well. A recombinant HIV antigen conjugated to colloidal gold embedded in the sample pad reacts with the HIV antibody present in serum or plasma forming conjugate / HIV antibody complex. As the mixture is allowed to migrate along the test strip, the conjugate / HIV antibody complex is captured by recombinant HIV antigen immobilized on a membrane forming a colored test band in the test region. A negative sample does not produce a test band due to the absence of colloidal gold conjugate / HIV antibody complex. The antigens used in the conjugate test are recombinant proteins that correspond to highly immunoreactive regions of HIV 1 and HIV 2. A colored control band in the control region appears at the end of test procedure regardless of test result. This control band is the result of colloidal gold conjugate binding to the anti-HIV antibody immobilized on the membrane. The…How reliable are Lateral flowimmunochromatographic assays when compared to molecular diagnostic tests or other serological tests? Please discuss referring to original articles example of covid 19..
- what are some advantages to using latex particles instead of blood cells for IM assay?Counterstaining with Hematoxylin and Eosin is an important step in which of the following techniques? ELISA Gel Electrophoresis IHC Western blottingFollowing is the data and notice that it is a terrible idea to culture hMSCs longer than 10 days. You’re strongly Days # cells0 50001 75002 125003 125004 218005 287006 530007 1143008 1653009 19200010 19200011 11680012 8950013 8830014 78300 Part1 You are working for a start-up that is pursuing a clinical trial. The trial involves grafting hMSCs intopatients suffering from interveterbral disc disease using a degradable polymer scaffold. You are going to 3Dprint a porous cylindrical scaffold that is 2 cm in radius and 1 cm in height (matching the dimensions of adegenerated disc). Assume a porosity of 50%. You will fill available volume of the scaffold with hMSCs at adensity of 1 million cells per cm3. Based on the data above, what starting number of cells will you use andhow long will it take you to get enough cells for the trial? Part2The trial is a failure (patients did not report any reduction in back pain). Your team wants to try againusing 85% hMSCs and 15% nucleus pulposus cells .…
- Please DESCRIBE, in outline form, the method you will use to select for bacterial cells that have taken up the pL311 plasmid, and to screen those cells for the presence of plasmids that are likely to contain a cloned gene. Be sure to mention the specific media you will use. In addition, please explain the rationale behind this specific selection and screening procedure. (Remember that you have available the following types of media: (i) media containing neither kanamycin nor X-gal, (ii) media containing BOTH kanamycin and X-gal, (iii) media containing tetracycline, and (iv) media containing ampicillin.Transformation of mammalian cells with viruses and obtaining mammalian cells harboring the virus is a method used to maintain the virus of interest. During this process what are the plaques observed? A. Clear areas in a layer of cultured cells degenerated and lysed due to virus infection. B. Stained areas in a cell culture indicating cells infected by a virus. C. Virus colonies on agar. D. Bacterial colonies on agarHBs Antibody Principle: This test uses the “sandwich principle”, a slide phase colloidal gold enhanced immunoassay technique for determination of HBs antibody in human serum or plasma. The nitrocellulose membrane was immobilized with HBs antigen on the test band region and anti-HBsAg antibody on the control band region. During the assay, the specimen is allowed to react with the colored conjugate (HBs Ag colloid gold conjugate); the mixture then migrates chromatographically on the membrane by the capillary action. For a positive result, a color band with the specific antibody-HBsAg complex will form on the membrane. Absence of this colored band in the test band region suggests a negative result. To serve as a procedural control, a colored band at control region always appears in the test area. The reagent contains scavenge antibodies to reduce nonspecific reactivity in human serum or plasma specimens. The sensitivity of the test is 10mIU. HBs Antigen Principle:…
- You are counting plaques on your plaque assay plates made from serial dilutions of your high titer lysate. Your 10-5 plate has 615 plaques although some are butting up against each other so it is difficult to get an accurate count. Your 10-6 plate has 42 plaques, and your 10-7 plate has only 1 plaque. Which plate would probably yield the most accurate titer calculation of your phage and why is it more trustworthy than the others?How can we easily determine VEGF expression in a western blot experiment? By using fluorescent microscopy to view its transport into the cell By using a primary antibody targeting VEGF By adding purified VEGFR to an SDS polyacrylamide gel By doing a mass spectrometry analysisExplain the statistical findings regarding the lateral flowimmunochromatographic assays sensitivity and robustness when compared to other test methods? Please discuss refering to original articles as examples Covid 19.