Non-conventional genetic engineering transformation method for transfer of wheat lysine genes in to common bean genome

Q: How can DNA microarrays be used to determine which genes of a bacterial pathogen are expressed both…

A: DNA microarray or biochip is a technique wherein different DNA molecules are first attached to a…

Q: Why must the gene be inserted into a vector for it to be cloned?

A: Vectors are carriers of target DNA which are readily used in recombinant DNA technology. The most…

Q: Identify the correct sequence of steps involved in cutting and reassembling DNA molecules to make…

A: Answer: Recombinant DNA is the DNA which is made in the laboratory  by combining two genetic…

Q: Which statement is NOT the function of restriction enzyme?

A: Answer: It ligates the desired to gene to vector’s plasmid.

Q: Enzymes of bacterial origin used in a wide variety of techniques are: ligases restriction…

A: DNA polymerase

Q: Why is this technique used?

A: All cells must synthesize some proteins for their cellular function. The instruction for protein…

Q: What size would the PCR product be for a different chromosome where there were nine tandem repeats.

A: The each STR is 5 bp. So, in the given diagram the total length of the PCR product is 420 bp. It…

Q: In 1996, a group of scientists from Scotland used a somatic cell of an adult sheep and fused it with…

A: Cloning

Q: In a specific step for PCR, the single-stranded DNA attaches x to primer sequences complementary to…

A: The steps of PCR are - Step 1: Denaturation The DNA double helix separates due to rise in…

Q: Not make to copy or incorrectly do. Else downvote.

A: We know that Genotype is defined as the genetic makeup of an individual organism and it is the sum…

Q: What color colonies will cells that contain a recombinant plasmid form?

A: Recombinant plasmids are the vectors that carry target DNA or gene sequence to the host by the help…

Q: Explain how DNA probes with different fluorescence emissionwavelengths can be used in a single FISH…

A: The FISH (fluorescence in situ hybridization) experiment uses a probe that is fluorescently labeled…

Q: principle of extractio of genomic DNA from bacterial sample

A: The technique of extracting and isolating DNA from a tissue or cells is known as DNA extraction. The…

Q: After transformation you were asked to grow bacterial cells transformed with plasmid on a plate that…

A: Bacterial transformation is a process through which bacteria can take up foreign DNA. Transformation…

Q: Draw a diagram from gel electrophoresis that illustrates the result of Sanger Sequencing, label…

A:

Q: List the steps in the polymerase chain reaction; discuss one disadvantage to this technique

A: Introduction Almost all the molecular biology techniques revolve around DNA/RNA/Proteins. DNA is one…

Q: You are cloning a gene from a eukaryotic organism into a plasmid to produce a recombinant protein in…

A: The biological process by which naked DNA (deoxyribonucleic acid) gets transferred from one…

Q: The yeast genome has class 1 elements (Ty1, Ty2, and so forth) but no class 2 elements. What is a…

A: Yeast has a very compact genome having 70 % of its DNA as exons, therefore it contains insufficient…

Q: natural crops (non-GMO) and transgenic organism.

A: Natural crops are those crops which are grown naturally. These crops exists in our surrounding from…

Q: What is not true for Sequence tagged site (STS) markers: cannot be mapped by fluorescence in situ…

A:

Q: Record the type of mutation obtained for the mutagens. Mutagen Alkylator.  Was it point, addition,…

A: Mutagens are physical or chemical agents that induce mutation in the DNA (deoxyribonucleic acid).…

Q: Primer role in PCR

A: Two primers are utilized, one for each of the complementary single strands of DNA released during…

Q: somatic cells and CRISPR Cas9

A: Cells are the littlest units of life, and subsequently are regularly alluded to as the "building…

Q: Differentiate between TWO of the following pairs: Genetic and a restriction map Southern and…

A: The mapping of DNA refers to the identification of the location of particular genes. Genetic map and…

Q: Which of the following technologies can best differentiate between a diploid wild banana and a…

A: Edible banana have either 22 , 33 or 44 chromosomes that represent diploid , triploid and…

Q: What needs to happen so the reporter molecule can emit fluorescence that is not quenched?

A: Real time PCR is a technique used to monitor and quantify the PCR product reaction in real time. The…

Q: how to make extract DNA through banana

A: DNA is a nucleic acid that is organized into chromosomes. It contains genetic information. It is…

Q: Genes of same species, similarly related to each other area) Orthologb) Syntenyc) Paralogd)…

A: DNA is the genetic material in most living organisms. It is the information hub of the cell that…

Q: During the differential sene expression module.which of the following was used as template in the…

A: Differential gene expression is the process through which cells differ from one another because of…

Q: Recombinant bacteria can produce hormones that are normally produced in humans. Briefly describe how…

A: Recombinant bacteria or genetically modified bacteria are produced when a gene of interest is…

Q: Write why are you extracting DNA of fruit

A: Deoxyribonucleic acid (DNA) is a molecule made out of two polynucleotide chains that curl around one…

Q: WHOLE CHROMOSOME ARE NOT ANALYZED IN DNA FINGERPRINTING METHOD.

A: Deoxy ribonucleic acid or DNA fingerprinting is a way to identify a certain individual rather than…

Q: Technique whereby inserting DNA into a clone is accomplished using two different restriction enzymes

A: Directional cloning is the technique which is accomplished by cleaving the plasmid vector with two…

Q: expalin Yeast centromeric DNA sequences

A: Centromere, also known as kinetochores are DNA sequences that are involved in linking a pair of…

Q: Are you a hidden heterozygote? A PCR analysis (part2) Agarose gel electrophoresis and interpretation…

A: Agarose gel concentration  More the concentration of the agarose gel more smaller will be its pores.…

Q: Explain the function of reverse transcriptase enzyme.

A: Enzymes are the biocatalyst that enhances the rate of reaction. They decrease the activation energy…

Q: Correct order ib which the following enzynes would operate to fix a damaged nucleotide in a human…

A: The correct order of enzymes used to fix a damaged nucleotide in a human gene is option d , i.e.,…

Q: 2. Guapple (Large sized guava) 3. Human Insulin- producing bacteria 4. Flavr-Savr (Delayed- ripening…

A: The activity is done below.

Q: The function of ligase is toa. rejoin segments of DNAb. make longitudinal cuts in DNAc. synthesize…

A: Introduction  DNA replication is very crucial process for the continuation of life as every new…

Q: clones produced from the same organism are found to exhibit slightly different physical traits. what…

A: Cloning is defined as the process by which cells, products, or individuals having identical or…

Q: Separate the word into their word parts and then define each word part. Mutagen

A: Mutaion is where the DNA or any viral RNA is altered by some external factors which may or may not…

Q: Fig. 1 shows a simple illustration how recombinant DNA are made. DNA sequence of interest DNA of…

A: The picture describes the process of making recombinant DNA. A recombinant DNA is a DNA molecule…

Q: In part 2 you ran your samples on an agarose gel. Explain how agarose gel electrophoresis works…

A: Gel electrophoresis is a technique used to separate DNA fragments according to their size. DNA…

Q: Taq Polymerase is extracted from which of the living organism, why we can extract only from this…

A: Taq polymerase is a DNA polymerase I enzyme that is utilized in the polymerase chain reaction (PCR)…

Q: There are a range of ethical issues associated with cloning. However, many of these are not applied…

A: Ethics of cloning are the set of guidelines and responsibilities of cloning which the researcher or…

Q: A researcher is doing PCR to have more of HDR template which will be used further for yeast…

A: PCR is the polymerase chain reaction in which using the polymerase enzyme from Thermus aquaticus…

Q: Could I get a background on the repo gene (reversed polarity

A: repo gene is reversed polarity gene that is abundantly studied in Drosophila melanogaster.

Q: Whole chromosome are not analyzed in DNA fingerprinting method. Explain.

A: Introduction: DNA is a genetic materiel of living organism except in some viruses. DNA…

Q: Explain why crops such as bananas, seedless watermelons, and potatoes are mutants

A: A mutation is the alternation of nucleotide sequence in the genome of an organism. To improve the…

Q: When the genetic materials are improperly replicated and error cannot be corrected the cell proceeds…

A: Cell cycle is the process by which a cell grows and divides to produce daughter cells. It can be…