quadrants contain leading strands. left When DNA pol III on RNA primer, thus running out of template, it leaves a single- stranded gap between the last DNA nucleotide of the newly synthesized daughter strand and the first nucleotide of the RNA primer (Figure 7.19). The pol III, having very low affin- ity for these DNA-RNA single-stranded gaps, is then replaced by DNA polymerase I (DNA pol I), which has high affin- ity for such gaps (Figure 7.19,). The DNA pol I removes nucleotides of the RNA primer one with DNA nucleotides, beginning with the 5' nucleotide of the RNA primer and progressing in the 3' direction until all the RNA nucleotides in the primer have been replaced by DNA nucleotides complementary to the template strand. The daughter strands in the upper left and lower right quadrants shown in Figure 7.18 have a 5'-to-3' direction of elongation that runs opposite to the direction of movement of the replication fork. These daughter strands are elongated discontinuously, in short segments, each of which is initiated by an RNA primer. The discontinuously synthesized daughter strand is called the lagging strand. Thus in Figure 7.18, the lower right and upper left quadrants of the replication bubble contain lagging strands (see also step 5 of Figure 7.14) Reiji Okazaki detected the synthesis of short fragments of DNA in the replication of the lagging strand. He observed by one and replaces them Okazaki Figure 7.18 The replication bubble. Bidirectional expansion is driven by DNA synthesis at each replication fork. One repli- some containing two DNA pol III enzymes operates at each fork to replicate both daughter strands. Template DNA fragments Daughter DNA oric Replisome region Replisome region 1 3' 5 Replication fork 3 (location of Replication fork (location of one replisome) 3' 3'5' 5' 3' one replisome) 5' RNA primer Draw a second replication 5' bubble to the right of the 3' Leading strand Lagging strand T one illustrated. As these two Lagging strand 5' 3' replication bubbles expand toward one another, what kind of strand will each leading Leading strand 3' 5' 3' 3 5' 3' 5' i3' strand encounter when the bubbles make contact? 2 1 Okazaki fragments baot De 10 oric Bidirectional expansion of bubble d n0 in in

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter28: Dna Metabolism: Replication, Recombination, And Repair
Section: Chapter Questions
Problem 18P: Functional Consequences of Y-Family DNA Polymerase Structure The eukaryotic translesion DNA...
icon
Related questions
Question

Please help me with the orange question. 

My answer is leading strand will encounter lagging strand. 

I dont know if it’s correct.

Thank you

quadrants contain leading strands.
left
When DNA pol III on
RNA primer, thus running out of template, it leaves a single-
stranded gap between the last DNA nucleotide of the newly
synthesized daughter strand and the first nucleotide of the
RNA primer (Figure 7.19). The pol III, having very low affin-
ity for these DNA-RNA single-stranded gaps, is then replaced
by DNA polymerase I (DNA pol I), which has high affin-
ity for such gaps (Figure 7.19,). The DNA pol I removes
nucleotides of the RNA primer one
with DNA nucleotides, beginning with the 5' nucleotide of
the RNA primer and progressing in the 3' direction until all
the RNA nucleotides in the primer have been replaced by
DNA nucleotides complementary to the template strand.
The daughter strands in the upper left and lower right
quadrants shown in Figure 7.18 have a 5'-to-3' direction of
elongation that runs opposite to the direction of movement
of the replication fork. These daughter strands are elongated
discontinuously, in short segments, each of which is initiated
by an RNA primer. The discontinuously synthesized daughter
strand is called the lagging strand. Thus in Figure 7.18, the
lower right and upper left quadrants of the replication bubble
contain lagging strands (see also step 5 of Figure 7.14)
Reiji Okazaki detected the synthesis of short fragments
of DNA in the replication of the lagging strand. He observed
by
one and replaces them
Okazaki
Figure 7.18 The replication
bubble. Bidirectional expansion
is driven by DNA synthesis at
each replication fork. One repli-
some containing two DNA pol III
enzymes operates at each fork to
replicate both daughter strands.
Template DNA
fragments
Daughter DNA
oric
Replisome region
Replisome region
1
3' 5
Replication fork
3
(location of
Replication fork
(location of
one replisome)
3'
3'5'
5'
3'
one replisome)
5'
RNA primer
Draw a second replication
5'
bubble to the right of the
3'
Leading strand
Lagging strand
T
one illustrated. As these two
Lagging strand
5'
3'
replication bubbles expand
toward one another, what kind
of strand will each leading
Leading strand
3'
5'
3'
3
5' 3'
5' i3'
strand encounter when the
bubbles make contact?
2
1
Okazaki fragments
baot
De 10
oric
Bidirectional expansion
of bubble
d n0
in
in
Transcribed Image Text:quadrants contain leading strands. left When DNA pol III on RNA primer, thus running out of template, it leaves a single- stranded gap between the last DNA nucleotide of the newly synthesized daughter strand and the first nucleotide of the RNA primer (Figure 7.19). The pol III, having very low affin- ity for these DNA-RNA single-stranded gaps, is then replaced by DNA polymerase I (DNA pol I), which has high affin- ity for such gaps (Figure 7.19,). The DNA pol I removes nucleotides of the RNA primer one with DNA nucleotides, beginning with the 5' nucleotide of the RNA primer and progressing in the 3' direction until all the RNA nucleotides in the primer have been replaced by DNA nucleotides complementary to the template strand. The daughter strands in the upper left and lower right quadrants shown in Figure 7.18 have a 5'-to-3' direction of elongation that runs opposite to the direction of movement of the replication fork. These daughter strands are elongated discontinuously, in short segments, each of which is initiated by an RNA primer. The discontinuously synthesized daughter strand is called the lagging strand. Thus in Figure 7.18, the lower right and upper left quadrants of the replication bubble contain lagging strands (see also step 5 of Figure 7.14) Reiji Okazaki detected the synthesis of short fragments of DNA in the replication of the lagging strand. He observed by one and replaces them Okazaki Figure 7.18 The replication bubble. Bidirectional expansion is driven by DNA synthesis at each replication fork. One repli- some containing two DNA pol III enzymes operates at each fork to replicate both daughter strands. Template DNA fragments Daughter DNA oric Replisome region Replisome region 1 3' 5 Replication fork 3 (location of Replication fork (location of one replisome) 3' 3'5' 5' 3' one replisome) 5' RNA primer Draw a second replication 5' bubble to the right of the 3' Leading strand Lagging strand T one illustrated. As these two Lagging strand 5' 3' replication bubbles expand toward one another, what kind of strand will each leading Leading strand 3' 5' 3' 3 5' 3' 5' i3' strand encounter when the bubbles make contact? 2 1 Okazaki fragments baot De 10 oric Bidirectional expansion of bubble d n0 in in
Expert Solution
trending now

Trending now

This is a popular solution!

steps

Step by step

Solved in 3 steps with 1 images

Blurred answer
Knowledge Booster
Gene expression
Learn more about
Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.
Similar questions
  • SEE MORE QUESTIONS
Recommended textbooks for you
Biochemistry
Biochemistry
Biochemistry
ISBN:
9781305577206
Author:
Reginald H. Garrett, Charles M. Grisham
Publisher:
Cengage Learning
Biology Today and Tomorrow without Physiology (Mi…
Biology Today and Tomorrow without Physiology (Mi…
Biology
ISBN:
9781305117396
Author:
Cecie Starr, Christine Evers, Lisa Starr
Publisher:
Cengage Learning