DNA from 2 different sources 5: 5': 3' 5' EcoRI recognition sequence Incubate both DNAS with EcoRI, which cuts the DNA backbone between Gand A A "sticky 3 end" 5' 5: A "sticky 3' 3' :5' 5' end" Incubate the DNAS together, allowing sticky ends to hydrogen bond. 5° 3' 3: :5' Add DNA ligase, which covalently links the DNA backbones. Covalent bond 3' Covalent bond A recombinant DNA molecule FIGURE 21.1 The action of a restriction enzyme and the production of recombinant DNA. The restriction enzyme EcoRI binds to a spe- cific sequence, in this case 5'-GAATTC-3'. It cleaves the DNA backbone between G and A, producing DNA fragments. The single-stranded ends of different DNA fragments hydrogen bond with each other, because they have complementary sequences. DNA ligase catalyzes the formation of covalent bonds in the DNA backbones of the fragments. =ダ:ー= in 는4 FO:G
DNA from 2 different sources 5: 5': 3' 5' EcoRI recognition sequence Incubate both DNAS with EcoRI, which cuts the DNA backbone between Gand A A "sticky 3 end" 5' 5: A "sticky 3' 3' :5' 5' end" Incubate the DNAS together, allowing sticky ends to hydrogen bond. 5° 3' 3: :5' Add DNA ligase, which covalently links the DNA backbones. Covalent bond 3' Covalent bond A recombinant DNA molecule FIGURE 21.1 The action of a restriction enzyme and the production of recombinant DNA. The restriction enzyme EcoRI binds to a spe- cific sequence, in this case 5'-GAATTC-3'. It cleaves the DNA backbone between G and A, producing DNA fragments. The single-stranded ends of different DNA fragments hydrogen bond with each other, because they have complementary sequences. DNA ligase catalyzes the formation of covalent bonds in the DNA backbones of the fragments. =ダ:ー= in 는4 FO:G
Biology: The Unity and Diversity of Life (MindTap Course List)
15th Edition
ISBN:9781337408332
Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr
Chapter8: Dna Structure And Function
Section: Chapter Questions
Problem 2CT: Review Figures 8.12 and 8.13. In cells, the primers for DNA synthesis are short strands of RNA, so...
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Gene Interactions
When the expression of a single trait is influenced by two or more different non-allelic genes, it is termed as genetic interaction. According to Mendel's law of inheritance, each gene functions in its own way and does not depend on the function of another gene, i.e., a single gene controls each of seven characteristics considered, but the complex contribution of many different genes determine many traits of an organism.
Gene Expression
Gene expression is a process by which the instructions present in deoxyribonucleic acid (DNA) are converted into useful molecules such as proteins, and functional messenger ribonucleic (mRNA) molecules in the case of non-protein-coding genes.
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Question
Prior to the action of DNA ligase, how many hydrogen bonds are holding these two DNA fragments together?
Transcribed Image Text:DNA from 2 different sources
5:
5':
3'
5'
EcoRI recognition
sequence
Incubate both DNAS with EcoRI,
which cuts the DNA backbone
between Gand A
A "sticky
3 end"
5'
5:
A "sticky
3'
3'
:5'
5'
end"
Incubate the DNAS
together, allowing sticky
ends to hydrogen bond.
5°
3'
3:
:5'
Add DNA ligase, which
covalently links the
DNA backbones.
Covalent bond
3'
Covalent bond
A recombinant DNA molecule
FIGURE 21.1 The action of a restriction enzyme and the production of recombinant DNA. The restriction enzyme EcoRI binds to a spe-
cific sequence, in this case 5'-GAATTC-3'. It cleaves the DNA backbone between G and A, producing DNA fragments. The single-stranded ends of
different DNA fragments hydrogen bond with each other, because they have complementary sequences. DNA ligase catalyzes the formation of covalent
bonds in the DNA backbones of the fragments.
=ダ:ー=
in
는4
FO:G
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